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HAMAP rule MF_01084

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General rule information [?]

Accession MF_01084
Dates 3-OCT-2003 (Created)
28-JUL-2023 (Last updated, Version 25)
Name Diphthine_synth
Scope(s) Archaea
Template(s) O58456 (DPHB_PYRHO); Q9YDI2 (DPHB_AERPE); [ Recover all ]
Triggered by HAMAP; MF_01084 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DPHB
Protein name RecName: Full=Diphthine synthase;
AltName: Full=Diphthamide biosynthesis methyltransferase;
Gene name Name=dphB;

Comments [?]

FUNCTIONS-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.
CATALYTIC ACTIVITY Reaction=2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine- [translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:36415, Rhea:RHEA-COMP:9749, Rhea:RHEA- COMP:10172, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73995, ChEBI:CHEBI:82696; EC=;
PATHWAYProtein modification; peptidyl-diphthamide biosynthesis.
SIMILARITYBelongs to the diphthine synthase family.

Keywords [?]

Gene Ontology [?]

GO:0004164; Molecular function:diphthine synthase activity
GO:0017183; Biological process:protein histidyl modification to diphthamide

Cross-references [?]

Pfam PF00590; TP_methylase; 1;
NCBIfam TIGR00522; Dph5; 1;
PIRSF PIRSF036432; Diphthine_synth; 1;

Features [?]

From: DPHB_PYRHO (O58456)
Key From To Description Tag Condition FTGroup
BINDING 115 116 /ligand="S-adenosyl-L-methionine"
BINDING 10 10 /ligand="S-adenosyl-L-methionine"
BINDING 87 87 /ligand="S-adenosyl-L-methionine"
BINDING 90 90 /ligand="S-adenosyl-L-methionine"
BINDING 166 166 /ligand="S-adenosyl-L-methionine"
BINDING 209 209 /ligand="S-adenosyl-L-methionine"
BINDING 234 234 /ligand="S-adenosyl-L-methionine"

Additional information [?]

Size range 206-300 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Possible wrong C-terminal sequence in THEVO

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