HAMAP rule MF_01084
General rule information
[?]
Accession |
MF_01084 |
Dates |
3-OCT-2003 (Created) 19-NOV-2022 (Last updated, Version 23) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
|
Full=Diphthine synthase;
|
|
EC 2.1.1.98;
|
AltName:
|
Full=Diphthamide biosynthesis methyltransferase;
|
|
Function |
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. |
Catalytic activity |
RHEA:36415: 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine
EC 2.1.1.98
|
Pathway |
Protein modification; peptidyl-diphthamide biosynthesis. |
Subunit |
Homodimer. |
Similarity |
Belongs to the diphthine synthase family. |
GO:0004164; Molecular function: diphthine synthase activity.
GO:0017183; Biological process: peptidyl-diphthamide biosynthetic process from peptidyl-histidine.
From: DPHB_PYRHO (O58456) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
BINDING
|
|
115
|
|
116
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[STN]-[IGVA]
|
|
|
BINDING (Optional)
|
|
10
|
|
10
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[LI]
|
|
|
BINDING
|
|
87
|
|
87
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
D
|
|
|
BINDING
|
|
90
|
|
90
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[VALMTIG]
|
|
|
BINDING
|
|
166
|
|
166
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[LIV]
|
|
|
BINDING
|
|
209
|
|
209
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[ALVM]
|
|
|
BINDING (Optional)
|
|
234
|
|
234
|
|
/ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789
|
|
|
|
[HYQ]
|
|
|
Additional information
[?]
Size range |
206-300 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Possible wrong C-terminal sequence in THEVO |