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HAMAP rule MF_01084

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General rule information [?]

Accession MF_01084
Dates 3-OCT-2003 (Created)
19-NOV-2022 (Last updated, Version 23)
Name Diphthine_synth
Templates O58456 (DPHB_PYRHO); Q9YDI2 (DPHB_AERPE): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Diphthine synthase;
AltName: Full=Diphthamide biosynthesis methyltransferase;
Gene name

Comments [?]

Function S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.
Catalytic activity RHEA:36415: 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine
Pathway Protein modification; peptidyl-diphthamide biosynthesis.
Subunit Homodimer.
Similarity Belongs to the diphthine synthase family.

Keywords [?]

Gene Ontology [?]

GO:0004164; Molecular function: diphthine synthase activity.
GO:0017183; Biological process: peptidyl-diphthamide biosynthetic process from peptidyl-histidine.

Cross-references [?]

Pfam PF00590; TP_methylase; 1;
TIGRFAMs TIGR00522; Dph5; 1;
PIRSF PIRSF036432; Diphthine_synth; 1;

Features [?]

From: DPHB_PYRHO (O58456)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     115     116       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [STN]-[IGVA]  
BINDING (Optional)     10     10       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [LI]  
BINDING     87     87       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     D  
BINDING     90     90       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [VALMTIG]  
BINDING     166     166       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [LIV]  
BINDING     209     209       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [ALVM]  
BINDING (Optional)     234     234       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [HYQ]  

Additional information [?]

Size range 206-300 amino acids
Related rules None
Fusion None
Comments Possible wrong C-terminal sequence in THEVO