HAMAP rule MF_01102
General rule information
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| Accession | MF_01102 |
| Dates | 16-JUL-2002 (Created)
1-JUN-2023 (Last updated, Version 33) |
| Name | MnmC |
| Scope(s) |
Bacteria Pseudomonadota Spirochaetota |
| Template(s) | P77182 (MNMC_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01102 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MNMC |
| Protein name | RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC; Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein; Includes: RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase; EC=2.1.1.61; Includes: RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase; EC=1.5.-.-; |
| Gene name | Name=mnmC; |
Comments
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| FUNCTION | Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34. |
| CATALYTIC ACTIVITY | Reaction=5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L- methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:19569, Rhea:RHEA- COMP:10195, Rhea:RHEA-COMP:10197, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74454, ChEBI:CHEBI:74455; EC=2.1.1.61; |
| COFACTOR | Name=FAD; Xref=ChEBI:CHEBI:57692; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family. |
| SIMILARITY | In the C-terminal section; belongs to the DAO family. |
Keywords
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| Cytoplasm |
| FAD |
| Flavoprotein |
| Methyltransferase |
| Multifunctional enzyme |
| Oxidoreductase |
| S-adenosyl-L-methionine |
| Transferase |
| tRNA processing |
Gene Ontology
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| GO:0050660; Molecular function:flavin adenine dinucleotide binding |
| GO:0016491; Molecular function:oxidoreductase activity |
| GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity |
| GO:0002097; Biological process:tRNA wobble base modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: MNMC_ECOLI (P77182) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 245 | /note="tRNA (mnm(5)s(2)U34)-methyltransferase" | |||||||||
| REGION | 270 | Cter | /note="FAD-dependent cmnm(5)s(2)U34 oxidoreductase" | |||||||||
Additional information
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| Size range | 585-754 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |