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HAMAP rule MF_01102

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General rule information [?]

Accession MF_01102
Dates 16-JUL-2002 (Created)
1-JUN-2023 (Last updated, Version 33)
Name MnmC
Scope(s) Bacteria
Template(s) P77182 (MNMC_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01102 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MNMC
Protein name RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC;
                 Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein;
RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase;
RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase;
Gene name Name=mnmC;

Comments [?]

FUNCTIONCatalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.
CATALYTIC ACTIVITY Reaction=5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L- methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:19569, Rhea:RHEA- COMP:10195, Rhea:RHEA-COMP:10197, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74454, ChEBI:CHEBI:74455; EC=;
SIMILARITYIn the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
SIMILARITYIn the C-terminal section; belongs to the DAO family.

Keywords [?]

Gene Ontology [?]

GO:0050660; Molecular function:flavin adenine dinucleotide binding
GO:0016491; Molecular function:oxidoreductase activity
GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity
GO:0002097; Biological process:tRNA wobble base modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01266; DAO; 1;
Pfam PF05430; Methyltransf_30; 1;
NCBIfam TIGR03197; MnmC_Cterm; 1;

Features [?]

From: MNMC_ECOLI (P77182)
Key From To Description Tag Condition FTGroup
REGION Nter 245 /note="tRNA (mnm(5)s(2)U34)-methyltransferase"
REGION 270 Cter /note="FAD-dependent cmnm(5)s(2)U34 oxidoreductase"

Additional information [?]

Size range 585-754 amino acids
Related rules None
Fusion Nter: None Cter: None

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