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HAMAP rule MF_01102

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General rule information [?]

Accession MF_01102
Dates 16-JUL-2002 (Created)
17-FEB-2023 (Last updated, Version 32)
Name MnmC
Bacteria; Pseudomonadota
Bacteria; Spirochaetota
Template P77182 (MNMC_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC;
Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein;
RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase;
RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase;
EC 1.5.-.-;
Gene name

Comments [?]

Function Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.
Catalytic activity RHEA:19569: 5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine
Cofactor FAD
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
In the C-terminal section; belongs to the DAO family.

Keywords [?]

Gene Ontology [?]

GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0002097; Biological process: tRNA wobble base modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01266; DAO; 1;
PF05430; Methyltransf_30; 1;
TIGRFAMs TIGR03197; MnmC_Cterm; 1;

Features [?]

From: MNMC_ECOLI (P77182)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     245       tRNA (mnm(5)s(2)U34)-methyltransferase        
REGION     270     Cter       FAD-dependent cmnm(5)s(2)U34 oxidoreductase        

Additional information [?]

Size range 585-754 amino acids
Related rules None
Fusion None