HAMAP rule MF_01102

General rule information [?]

Accession	MF_01102
Dates	16-JUL-2002 (Created) 17-FEB-2023 (Last updated, Version 32)

Name

MnmC

Scope

Bacteria; Pseudomonadota

Bacteria; Spirochaetota

Template

P77182 (MNMC_ECOLI)

Triggered by

HAMAP; MF_01102 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MNMC

Protein name

RecName:		Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC;
		Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein;
Includes:
	RecName:		Full=tRNA (mnm(5)s(2)U34)-methyltransferase;
			EC 2.1.1.61;
Includes:
	RecName:		Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase;
			EC 1.5.-.-;

Gene name

mnmC

Comments [?]

Function	Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.
Catalytic activity	RHEA:19569: 5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine EC 2.1.1.61
Cofactor	FAD
Subcellular location	Cytoplasm.
Similarity	In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
	In the C-terminal section; belongs to the DAO family.

Keywords [?]

Cytoplasm
FAD
Flavoprotein
Methyltransferase
Multifunctional enzyme
Oxidoreductase
S-adenosyl-L-methionine
Transferase
tRNA processing

Gene Ontology [?]

GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0002097; Biological process: tRNA wobble base modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01266; DAO; 1;
	PF05430; Methyltransf_30; 1;
TIGRFAMs	TIGR03197; MnmC_Cterm; 1;

Features [?]

From: MNMC_ECOLI (P77182)

Key From To Description Tag Condition FTGroup

REGION Nter 245 tRNA (mnm(5)s(2)U34)-methyltransferase

REGION 270 Cter FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Additional information [?]

Size range	585-754 amino acids
Related rules	None
Fusion	None

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