HAMAP rule MF_01102
General rule information
[?]
Accession | MF_01102 |
Dates | 16-JUL-2002 (Created) 17-FEB-2023 (Last updated, Version 32) |
Name | MnmC |
Scope | Bacteria; Pseudomonadota
Bacteria; Spirochaetota |
Template | P77182 (MNMC_ECOLI) |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Gene name |
|
Comments
[?]
Function | Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34. |
Catalytic activity | RHEA:19569: 5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine
EC 2.1.1.61 |
Cofactor | FAD |
Subcellular location | Cytoplasm. |
Similarity | In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family. |
In the C-terminal section; belongs to the DAO family. |
Keywords
[?]
Cytoplasm
FAD
Flavoprotein
Methyltransferase
Multifunctional enzyme
Oxidoreductase
S-adenosyl-L-methionine
Transferase
tRNA processing
FAD
Flavoprotein
Methyltransferase
Multifunctional enzyme
Oxidoreductase
S-adenosyl-L-methionine
Transferase
tRNA processing
Gene Ontology
[?]
GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0002097; Biological process: tRNA wobble base modification.
GO:0005737; Cellular component: cytoplasm.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0002097; Biological process: tRNA wobble base modification.
GO:0005737; Cellular component: cytoplasm.
Cross-references
[?]
Features
[?]
From: MNMC_ECOLI (P77182) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | Nter | 245 | tRNA (mnm(5)s(2)U34)-methyltransferase | |||||||||
REGION | 270 | Cter | FAD-dependent cmnm(5)s(2)U34 oxidoreductase |
Additional information
[?]
Size range | 585-754 amino acids |
Related rules | None |
Fusion | None |