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HAMAP rule MF_01113

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General rule information [?]

Accession MF_01113
Dates 7-JUN-2002 (Created)
17-JAN-2023 (Last updated, Version 43)
Name DNApol_IV
Scope
Bacteria
Archaea
Templates Q47155 (DPO4_ECOLI); P96022 (DPO4_SACSO); Q97W02 (DPO4_SACS2): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DPO4
Protein name
RecName: Full=DNA polymerase IV;
Short=Pol IV;
EC 2.7.7.7;
case <OC:Bacteria>
Gene name
dinB
end case
case <OC:Archaea>
Gene name
dbh
end case

Comments [?]

case <OC:Bacteria>
Function Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.
end case
case <OC:Archaea>
Function Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.
end case
Catalytic activity RHEA:22508: a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
EC 2.7.7.7
Cofactor Mg(2+)
Note: Binds 2 magnesium ions per subunit.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the DNA polymerase type-Y family.

Keywords [?]

Cytoplasm
Transferase
Nucleotidyltransferase
DNA-directed DNA polymerase
DNA replication
DNA damage
DNA repair
DNA-binding
Mutator protein
Metal-binding
Magnesium

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0003887; Molecular function: DNA-directed DNA polymerase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006261; Biological process: DNA-templated DNA replication.
GO:0006281; Biological process: DNA repair.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00817; IMS; 1;
PROSITE PS50173; UMUC; 1; trigger=PRU00216;

Features [?]

From: DPO4_SACSO (P96022)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     106     106             E  
BINDING     7     7       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
BINDING     105     105       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
SITE     12     12       Substrate discrimination        

Additional information [?]

Size range 318-479 amino acids
Related rules None
Fusion None


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