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HAMAP rule MF_01113
General rule information
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| Accession | MF_01113 |
| Dates | 7-JUN-2002 (Created)
17-JAN-2023 (Last updated, Version 43) |
| Name | DNApol_IV |
| Scope(s) |
Bacteria Archaea |
| Template(s) | Q47155 (DPO4_ECOLI); P96022 (DPO4_SACSO); Q97W02 (DPO4_SACS2); [ Recover all ] |
| Triggered by |
HAMAP; MF_01113 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DPO4 |
| Protein name | RecName: Full=DNA polymerase IV; Short=Pol IV; EC=2.7.7.7; |
| case <OC:Bacteria> | |
| Gene name | Name=dinB; |
| end case | |
| case <OC:Archaea> | |
| Gene name | Name=dbh; |
| end case | |
Comments
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| case <OC:Bacteria> | |
| FUNCTION | Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII. |
| end case | |
| case <OC:Archaea> | |
| FUNCTION | Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.7; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit.; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the DNA polymerase type-Y family. |
Keywords
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| Cytoplasm |
| Transferase |
| Nucleotidyltransferase |
| DNA-directed DNA polymerase |
| DNA replication |
| DNA damage |
| DNA repair |
| DNA-binding |
| Mutator protein |
| Metal-binding |
| Magnesium |
Gene Ontology
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| GO:0003677; Molecular function:DNA binding |
| GO:0003887; Molecular function:DNA-directed DNA polymerase activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0006261; Biological process:DNA-templated DNA replication |
| GO:0006281; Biological process:DNA repair |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: DPO4_SACSO (P96022) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 106 | 106 | E | |||||||||
| BINDING | 7 | 7 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 105 | 105 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| SITE | 12 | 12 | /note="Substrate discrimination" | |||||||||
Additional information
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| Size range | 318-479 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |