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HAMAP rule MF_01113

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General rule information [?]

Accession MF_01113
Dates 7-JUN-2002 (Created)
17-JAN-2023 (Last updated, Version 43)
Name DNApol_IV
Scope(s) Bacteria
Archaea
Template(s) Q47155 (DPO4_ECOLI); P96022 (DPO4_SACSO); Q97W02 (DPO4_SACS2); [ Recover all ]
Triggered by HAMAP; MF_01113 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DPO4
Protein name RecName: Full=DNA polymerase IV;
                 Short=Pol IV;
                 EC=2.7.7.7;
case <OC:Bacteria>
Gene name Name=dinB;
end case
case <OC:Archaea>
Gene name Name=dbh;
end case

Comments [?]

case <OC:Bacteria>
FUNCTIONPoorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.
end case
case <OC:Archaea>
FUNCTIONPoorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.
end case
CATALYTIC ACTIVITY Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.7;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit.;
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the DNA polymerase type-Y family.

Keywords [?]


Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
GO:0003887; Molecular function:DNA-directed DNA polymerase activity
GO:0000287; Molecular function:magnesium ion binding
GO:0006261; Biological process:DNA-templated DNA replication
GO:0006281; Biological process:DNA repair
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00817; IMS; 1;
PROSITE PS50173; UMUC; 1;

Features [?]

From: DPO4_SACSO (P96022)
Key From To Description Tag Condition FTGroup
ACT_SITE 106 106 E
BINDING 7 7 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D
BINDING 105 105 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D
SITE 12 12 /note="Substrate discrimination"

Additional information [?]

Size range 318-479 amino acids
Related rules None
Fusion Nter: None Cter: None



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