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Annotation rule MF_01123
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General rule information [?]

Accession MF_01123
Dates 24-SEP-2003 (Created)
19-NOV-2019 (Last updated, Version 27)
Name Ac_CoA_synth
Scope
Bacteria
Archaea
Templates P27550 (ACSA_ECOLI); Q8ZKF6 (ACSA_SALTY); P9WQD1 (ACSA_MYCTU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ACSA
Protein name
RecName: Full=Acetyl-coenzyme A synthetase;
Short=AcCoA synthetase;
Short=Acs;
EC 6.2.1.1;
AltName: Full=Acetate--CoA ligase;
AltName: Full=Acyl-activating enzyme;
case <OC:Enterobacterales>
Gene name
acs
else
Gene name
acsA
end case

Comments [?]

case <OC:Enterobacterales>
Function Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. Acs undergoes a two-step reaction. In the first half reaction, Acs combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.
Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis.
else
Function Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.
end case
Catalytic activity RHEA:23176: acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate
EC 6.2.1.1
Cofactor Mg(2+)
Ptm Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family.

Keywords [?]


Gene Ontology [?]

GO:0003987; Molecular function: acetate-CoA ligase activity.
case <OC:Enterobacterales>
GO:0019427; Biological process: acetyl-CoA biosynthetic process from acetate.
GO:0006935; Biological process: chemotaxis.
end case

Cross-references [?]

Pfam PF00501; AMP-binding; 1;
PRINTS PR00154; AMPBINDING; 1;
TIGRFAMs TIGR02188; Ac_CoA_lig_AcsA; 1;
PROSITE PS00455; AMP_BINDING; 1;

Features [?]

From: ACSA_SALTY (Q8ZKF6)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     387     389       ATP     G-[ES]-[PG]  
NP_BIND     411     416       ATP     [DS]-T-[WF]-W-[QM]-T  
REGION     191     194       Coenzyme A binding     [RY]-[AGRKN]-[GSNDP]-x  
METAL     537     537       Magnesium; via carbonyl oxygen     V  
METAL     539     539       Magnesium; via carbonyl oxygen     [HF]  
METAL     542     542       Magnesium; via carbonyl oxygen     [IV]  
BINDING (Optional)     311     311       Coenzyme A     [TS]  
BINDING (Optional)     335     335       Coenzyme A     N  
BINDING (Optional)     500     500       ATP     D  
BINDING (Optional)     515     515       ATP     R  
BINDING (Optional)     523     523       Coenzyme A; via carbonyl oxygen     S  
BINDING (Optional)     526     526       ATP     R  
BINDING (Optional)     584     584       Coenzyme A     R  
MOD_RES     609     609       N6-acetyllysine     K  

Additional information [?]

Size range 572-672 amino acids
Related rules None
Fusion None
Comments More divergent AcsA present in BACSU is not included in the alignment