HAMAP logo

HAMAP rule MF_01139

Send feedback

General rule information [?]

Accession MF_01139
Dates 1-SEP-2004 (Created)
13-JAN-2023 (Last updated, Version 35)
Name ISPT
Scope
Bacteria
Archaea
Templates P60472 (UPPS_ECOLI); O82827 (UPPS_MICLU); Q9HH76 (UPPS_SULAC); P9WFF7 (DPDS_MYCTU); A0R0S4 (DPDS_MYCS2); P9WFF5 (ZFPP_MYCTU); A0R2W4 (ZFPP_MYCS2); Q47SS3 (ZFPP_THEFY); Q47RM6 (DPDP_THEFY): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Gammaproteobacteria>
Identifier
UPPS
Protein name
RecName: Full=Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific);
EC 2.5.1.31;
AltName: Full=Ditrans,polycis-undecaprenylcistransferase;
AltName: Full=Undecaprenyl diphosphate synthase;
Short=UDS;
AltName: Full=Undecaprenyl pyrophosphate synthase;
Short=UPP synthase;
Gene name
uppS
else case <OC:Archaea>
Identifier
UPPS
Protein name
RecName: Full=Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific);
EC 2.5.1.89;
AltName: Full=Undecaprenyl diphosphate synthase;
Short=UDS;
AltName: Full=Undecaprenyl pyrophosphate synthase;
Short=UPP synthase;
Gene name
uppS
else
Identifier
ISPT
Protein name
RecName: Full=Isoprenyl transferase;
EC 2.5.1.-;
end case

Comments [?]

case <OC:Gammaproteobacteria>
Function Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di-trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.
Catalytic activity RHEA:27551: (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = di-trans,octa-cis-undecaprenyl diphosphate + 8 diphosphate
EC 2.5.1.31
else case <OC:Archaea>
Function Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.
Catalytic activity RHEA:27622: geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate
EC 2.5.1.89
else
Function Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.
end case
Cofactor Mg(2+)
Note: Binds 2 magnesium ions per subunit.
Subunit Homodimer.
Similarity Belongs to the UPP synthase family.

Keywords [?]

Magnesium
Metal-binding
Transferase
case <OC:Gammaproteobacteria>
Cell shape
Cell wall biogenesis/degradation
Peptidoglycan synthesis
end case

Gene Ontology [?]

case <OC:Gammaproteobacteria>
GO:0008834; Molecular function: di-trans,poly-cis-decaprenylcistransferase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
end case
GO:0000287; Molecular function: magnesium ion binding.
GO:0004659; Molecular function: prenyltransferase activity.

Cross-references [?]

Pfam PF01255; Prenyltransf; 1;
TIGRFAMs TIGR00055; UppS; 1;
PROSITE PS01066; UPP_SYNTHASE; 1;

Features [?]

From: UPPS_ECOLI (P60472)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     26     26             D  
ACT_SITE     74     74       Proton acceptor     N  
BINDING     27     30       /ligand="substrate     G-N-x-[RK]  
BINDING     71     73       /ligand="substrate     S-x-[ED]  
BINDING     200     202       /ligand="substrate     R-x-S  
BINDING     31     31       /ligand="substrate     [WF]  
BINDING     39     39       /ligand="substrate     [RK]  
BINDING     43     43       /ligand="substrate     H  
BINDING     75     75       /ligand="substrate     [WF]  
BINDING     77     77       /ligand="substrate     [RK]  
BINDING     194     194       /ligand="substrate     R  
BINDING     26     26       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
case <OC:Enterobacterales>
BINDING     199     199       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     H  
end case
BINDING     213     213       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E  

Additional information [?]

Size range 219-339 amino acids
Related rules None
Fusion Nter: <crtB>; Cter: None
Comments Actinomycetota possess different types of isoprenyl transferases (decaprenyl diphosphate synthase; trans,polycis-decaprenyl diphosphate synthase; (2Z,6E)-farnesyl diphosphate synthase; trans,polycis-polyprenyl diphosphate synthase) which are all homologous to ISPT in primary sequence (and match the family MF_01139) but with a different substrate specificity (isoprenyl acceptor) and chain-length determination due to a few residues.


View rule in raw text format (no links)