HAMAP rule MF_01139
General rule information
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Accession | MF_01139 |
Dates | 1-SEP-2004 (Created) 13-JAN-2023 (Last updated, Version 35) |
Name | ISPT |
Scope | Bacteria
Archaea |
Templates | P60472 (UPPS_ECOLI); O82827 (UPPS_MICLU); Q9HH76 (UPPS_SULAC); P9WFF7 (DPDS_MYCTU); A0R0S4 (DPDS_MYCS2); P9WFF5 (ZFPP_MYCTU); A0R2W4 (ZFPP_MYCS2); Q47SS3 (ZFPP_THEFY); Q47RM6 (DPDP_THEFY): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Gammaproteobacteria>
Identifier |
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Protein name |
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Gene name |
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else case <OC:Archaea>
Identifier |
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Protein name |
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Gene name |
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else
Identifier |
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Protein name |
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end case
Comments
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case <OC:Gammaproteobacteria>
Function | Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di-trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide. |
Catalytic activity | RHEA:27551: (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = di-trans,octa-cis-undecaprenyl diphosphate + 8 diphosphate
EC 2.5.1.31 |
else case <OC:Archaea>
Function | Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids. |
Catalytic activity | RHEA:27622: geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate
EC 2.5.1.89 |
else
Function | Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids. |
end case
Cofactor | Mg(2+) Note: Binds 2 magnesium ions per subunit. |
Subunit | Homodimer. |
Similarity | Belongs to the UPP synthase family. |
Keywords
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case <OC:Gammaproteobacteria>
end case
Gene Ontology
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case <OC:Gammaproteobacteria>
GO:0008834; Molecular function: di-trans,poly-cis-decaprenylcistransferase activity.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
end case
GO:0000287; Molecular function: magnesium ion binding.
GO:0004659; Molecular function: prenyltransferase activity.
GO:0004659; Molecular function: prenyltransferase activity.
Cross-references
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Features
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From: UPPS_ECOLI (P60472) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 26 | 26 | D | |||||||||
ACT_SITE | 74 | 74 | Proton acceptor | N | ||||||||
BINDING | 27 | 30 | /ligand="substrate | G-N-x-[RK] | ||||||||
BINDING | 71 | 73 | /ligand="substrate | S-x-[ED] | ||||||||
BINDING | 200 | 202 | /ligand="substrate | R-x-S | ||||||||
BINDING | 31 | 31 | /ligand="substrate | [WF] | ||||||||
BINDING | 39 | 39 | /ligand="substrate | [RK] | ||||||||
BINDING | 43 | 43 | /ligand="substrate | H | ||||||||
BINDING | 75 | 75 | /ligand="substrate | [WF] | ||||||||
BINDING | 77 | 77 | /ligand="substrate | [RK] | ||||||||
BINDING | 194 | 194 | /ligand="substrate | R | ||||||||
BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D |
case <OC:Enterobacterales>
end case
Additional information
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Size range | 219-339 amino acids |
Related rules | None |
Fusion | Nter: <crtB>; Cter: None |
Comments | Actinomycetota possess different types of isoprenyl transferases (decaprenyl diphosphate synthase; trans,polycis-decaprenyl diphosphate synthase; (2Z,6E)-farnesyl diphosphate synthase; trans,polycis-polyprenyl diphosphate synthase) which are all homologous to ISPT in primary sequence (and match the family MF_01139) but with a different substrate specificity (isoprenyl acceptor) and chain-length determination due to a few residues. |