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HAMAP rule MF_01139

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General rule information [?]

Accession MF_01139
Dates 1-SEP-2004 (Created)
1-MAR-2024 (Last updated, Version 37)
Name ISPT
Scope(s) Bacteria
Archaea
Template(s) P60472 (UPPS_ECOLI); O82827 (UPPS_MICLU); Q9HH76 (UPPS_SULAC); P9WFF7 (DPDS_MYCTU); A0R0S4 (DPDS_MYCS2); P9WFF5 (ZFPP_MYCTU); A0R2W4 (ZFPP_MYCS2); Q47SS3 (ZFPP_THEFY); Q47RM6 (DPDP_THEFY); [ Recover all ]
Triggered by HAMAP; MF_01139 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Gammaproteobacteria>
Identifier UPPS
Protein name RecName: Full=Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific);
                 EC=2.5.1.31;
AltName: Full=Ditrans,polycis-undecaprenylcistransferase;
AltName: Full=Undecaprenyl diphosphate synthase;
                 Short=UDS;
AltName: Full=Undecaprenyl pyrophosphate synthase;
                 Short=UPP synthase;
Gene name Name=uppS;
else case <OC:Archaea>
Identifier UPPS
Protein name RecName: Full=Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific);
                 EC=2.5.1.89;
AltName: Full=Undecaprenyl diphosphate synthase;
                 Short=UDS;
AltName: Full=Undecaprenyl pyrophosphate synthase;
                 Short=UPP synthase;
Gene name Name=uppS;
else
Identifier ISPT
Protein name RecName: Full=Isoprenyl transferase;
                 EC=2.5.1.-;
end case

Comments [?]

case <OC:Gammaproteobacteria>
FUNCTIONCatalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di- trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide.
CATALYTIC ACTIVITY Reaction=(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = di- trans,octa-cis-undecaprenyl diphosphate + 8 diphosphate; Xref=Rhea:RHEA:27551, ChEBI:CHEBI:33019, ChEBI:CHEBI:58405, ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.31;
else case <OC:Archaea>
FUNCTIONCatalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.
CATALYTIC ACTIVITY Reaction=geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate; Xref=Rhea:RHEA:27622, ChEBI:CHEBI:33019, ChEBI:CHEBI:57533, ChEBI:CHEBI:60388, ChEBI:CHEBI:128769; EC=2.5.1.89;
else
FUNCTIONCatalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit.;
SUBUNITHomodimer.
SIMILARITYBelongs to the UPP synthase family.

Keywords [?]

Magnesium
Metal-binding
Transferase
case <OC:Gammaproteobacteria>
Cell shape
Cell wall biogenesis/degradation
Peptidoglycan synthesis
end case

Gene Ontology [?]

case <OCellular component:Gammaproteobacteria>
GO:0008834; Molecular function:di-trans,poly-cis-undecaprenyl-diphosphate synthase activity
GO:0009252; Biological process:peptidoglycan biosynthetic process
end case
GO:0000287; Molecular function:magnesium ion binding
GO:0004659; Molecular function:prenyltransferase activity

Cross-references [?]

Pfam PF01255; Prenyltransf; 1;
NCBIfam TIGR00055; UppS; 1;
PROSITE PS01066; UPP_SYNTHASE; 1;

Features [?]

From: UPPS_ECOLI (P60472)
Key From To Description Tag Condition FTGroup
ACT_SITE 26 26 D
ACT_SITE 74 74 /note="Proton acceptor" N
BINDING 27 30 /ligand="substrate" G-N-x-[RK]
BINDING 71 73 /ligand="substrate" S-x-[ED]
BINDING 200 202 /ligand="substrate" R-x-S
BINDING 31 31 /ligand="substrate" [WF]
BINDING 39 39 /ligand="substrate" [RK]
BINDING 43 43 /ligand="substrate" H
BINDING 75 75 /ligand="substrate" [WF]
BINDING 77 77 /ligand="substrate" [RK]
BINDING 194 194 /ligand="substrate" R
BINDING 26 26 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
case <OC:Enterobacterales>
BINDING 199 199 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 H
end case
BINDING 213 213 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E

Additional information [?]

Size range 219-339 amino acids
Related rules None
Fusion Nter: <crtB> Cter: None
Comments Actinomycetota possess different types of isoprenyl transferases (decaprenyl diphosphate synthase; trans,polycis-decaprenyl diphosphate synthase; (2Z,6E)-farnesyl diphosphate synthase; trans,polycis-polyprenyl diphosphate synthase) which are all homologous to ISPT in primary sequence (and match the family MF_01139) but with a different substrate specificity (isoprenyl acceptor) and chain-length determination due to a few residues.



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