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Annotation rule MF_01182
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General rule information [?]

Accession MF_01182
Dates 24-NOV-2006 (Created)
19-NOV-2019 (Last updated, Version 24)
Name Cytochrom_C552
Scope
Bacteria; Bacteroidetes
Bacteria; Proteobacteria
Template P0ABK9 (NRFA_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NRFA
Protein name
RecName: Full=Cytochrome c-552;
EC 1.7.2.2;
AltName: Full=Ammonia-forming cytochrome c nitrite reductase;
Short=Cytochrome c nitrite reductase;
Flags: Precursor;
Gene name
nrfA

Comments [?]

Function Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process.
Catalytic activity RHEA:13089: 6 [Fe(III)cytochrome c] + 2 H2O + NH4(+) = 6 [Fe(II)cytochrome c] + 8 H(+) + nitrite
EC 1.7.2.2
case <FTGroup:2>
Cofactor Ca(2+)
Note: Binds 1 Ca(2+) ion per monomer.
end case
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
Cofactor heme
Note: Binds 5 heme groups covalently per monomer.
end case
Pathway Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location Periplasm.
Similarity Belongs to the cytochrome c-552 family.

Keywords [?]

case <FTGroup:2>
end case
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
end case
case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:2>
GO:0005509; Molecular function: calcium ion binding.
end case
case <FTGroup:1>
GO:0005506; Molecular function: iron ion binding.
end case
GO:0042279; Molecular function: nitrite reductase (cytochrome, ammonia-forming) activity.
GO:0006807; Biological process: nitrogen compound metabolic process.
GO:0055114; Biological process: oxidation-reduction process.
GO:0042597; Cellular component: periplasmic space.

Cross-references [?]

PROSITE PS51008; MULTIHEME_CYTC; 1;
Pfam PF02335; Cytochrom_C552; 1;

Computed features [?]

General Signal; -; 1; trigger=yes;

Features [?]

From: NRFA_ECOLI (P0ABK9)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     94     94       Iron (heme 3 axial ligand)     H   1
METAL     126     126       Iron (heme 1 axial ligand)     K   1
METAL     164     164       Iron (heme 2 axial ligand)     H   1
METAL     213     213       Iron (heme 3 axial ligand)     H   1
METAL     215     215       Calcium     E   2
METAL     216     216       Calcium; via carbonyl oxygen     Y   2
METAL     261     261       Calcium; via carbonyl oxygen     K   2
METAL     263     263       Calcium     Q   2
METAL     275     275       Iron (heme 5 axial ligand)     H   1
METAL     286     286       Iron (heme 4 axial ligand)     H   1
METAL     301     301       Iron (heme 2 axial ligand)     H   1
METAL     318     318       Iron (heme 5 axial ligand)     H   1
METAL     393     393       Iron (heme 4 axial ligand)     H   1
BINDING     122     122       Heme 1 (covalent)     C   3
BINDING     125     125       Heme 1 (covalent)     C   3
BINDING     160     160       Heme 2 (covalent)     C   4
BINDING     163     163       Heme 2 (covalent)     C   4
BINDING     209     209       Heme 3 (covalent)     C   5
BINDING     212     212       Heme 3 (covalent)     C   5
BINDING     216     216       Substrate     Y  
BINDING     264     264       Substrate     H  
BINDING     282     282       Heme 4 (covalent)     C   6
BINDING     285     285       Heme 4 (covalent)     C   6
BINDING     314     314       Heme 5 (covalent)     C   7
BINDING     317     317       Heme 5 (covalent)     C   7

Additional information [?]

Size range 463-538 amino acids
Related rules None
Fusion None