HAMAP rule MF_01182

General rule information [?]

Accession	MF_01182
Dates	24-NOV-2006 (Created) 26-JAN-2023 (Last updated, Version 32)

Name	Cytochrom_C552

Scope

Bacteria; Bacteroidota

Bacteria; Pseudomonadota

Template

P0ABK9 (NRFA_ECOLI)

Triggered by

HAMAP; MF_01182 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

NRFA

Protein name

RecName:		Full=Cytochrome c-552;
		EC 1.7.2.2;
AltName:		Full=Ammonia-forming cytochrome c nitrite reductase;
		Short=Cytochrome c nitrite reductase;
Flags:		Precursor;

Gene name

nrfA

Comments [?]

Function	Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process.
Catalytic activity	RHEA:13089: 6 Fe(III)-[cytochrome c] + 2 H2O + NH4(+) = 6 Fe(II)-[cytochrome c] + 8 H(+) + nitrite EC 1.7.2.2

case <FTGroup:2>

Cofactor

Ca(2+)
Note: Binds 1 Ca(2+) ion per monomer.

end case

case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>

Cofactor

heme c
Note: Binds 5 heme c groups covalently per monomer.

end case

Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location	Periplasm.
Similarity	Belongs to the cytochrome c-552 family.

Keywords [?]

case <FTGroup:2>

Calcium

end case

Electron transport

case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>

Heme

end case

case <FTGroup:1>

Iron
Metal-binding

end case

Oxidoreductase
Periplasm
Signal
Transport

Gene Ontology [?]

case <FTGroup:2>

GO:0005509; Molecular function: calcium ion binding.

end case

case <FTGroup:1>

GO:0005506; Molecular function: iron ion binding.

end case

GO:0042279; Molecular function: nitrite reductase (cytochrome, ammonia-forming) activity.
GO:0006807; Biological process: nitrogen compound metabolic process.
GO:0042597; Cellular component: periplasmic space.

Cross-references [?]

PROSITE	PS51008; MULTIHEME_CYTC; 1;
Pfam	PF02335; Cytochrom_C552; 1;

Computed features [?]

General

Signal; -; 1; trigger=yes;

Features [?]

From: NRFA_ECOLI (P0ABK9)

Key From To Description Tag Condition FTGroup

BINDING 94 94 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 126 126 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue K 1

BINDING 164 164 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 213 213 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 215 215 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 E 2

BINDING 216 216 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 Y 2

BINDING 261 261 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 K 2

BINDING 263 263 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 Q 2

BINDING 275 275 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 286 286 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 301 301 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 318 318 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 393 393 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue H 1

BINDING 122 122 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /note="covalent C 3

BINDING 125 125 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /note="covalent C 3

BINDING 160 160 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /note="covalent C 4

BINDING 163 163 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /note="covalent C 4

BINDING 209 209 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /note="covalent C 5

BINDING 212 212 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /note="covalent C 5

BINDING 216 216 /ligand="substrate Y

BINDING 264 264 /ligand="substrate H

BINDING 282 282 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /note="covalent C 6

BINDING 285 285 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /note="covalent C 6

BINDING 314 314 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /note="covalent C 7

BINDING 317 317 /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /note="covalent C 7

Additional information [?]

Size range	463-538 amino acids
Related rules	None
Fusion	None

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