HAMAP logo

HAMAP rule MF_01182

Send feedback

General rule information [?]

Accession MF_01182
Dates 24-NOV-2006 (Created)
26-JAN-2023 (Last updated, Version 32)
Name Cytochrom_C552
Scope(s) Bacteria
Bacteroidota
Pseudomonadota
Template(s) P0ABK9 (NRFA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01182 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NRFA
Protein name RecName: Full=Cytochrome c-552;
                 EC=1.7.2.2;
AltName: Full=Ammonia-forming cytochrome c nitrite reductase;
                 Short=Cytochrome c nitrite reductase;
                 Flags: Precursor;
Gene name Name=nrfA;

Comments [?]

FUNCTIONCatalyzes the reduction of nitrite to ammonia, consuming six electrons in the process.
CATALYTIC ACTIVITY Reaction=6 Fe(III)-[cytochrome c] + 2 H2O + NH4(+) = 6 Fe(II)- [cytochrome c] + 8 H(+) + nitrite; Xref=Rhea:RHEA:13089, Rhea:RHEA- COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:28938, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.7.2.2;
case <FTGroup:2>
COFACTOR Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 1 Ca(2+) ion per monomer.;
end case
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
COFACTOR Name=heme c; Xref=ChEBI:CHEBI:61717; Note=Binds 5 heme c groups covalently per monomer.;
end case
PATHWAYNitrogen metabolism; nitrate reduction (assimilation).
SUBCELLULAR LOCATIONPeriplasm.
SIMILARITYBelongs to the cytochrome c-552 family.

Keywords [?]

case <FTGroup:2>
Calcium
end case
Electron transport
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7>
Heme
end case
case <FTGroup:1>
Iron
Metal-binding
end case
Oxidoreductase
Periplasm
Signal
Transport

Gene Ontology [?]

case <FTGroup:2>
GO:0005509; Molecular function:calcium ion binding
end case
case <FTGroup:1>
GO:0005506; Molecular function:iron ion binding
end case
GO:0042279; Molecular function:nitrite reductase (cytochrome, ammonia-forming) activity
GO:0006807; Biological process:nitrogen compound metabolic process
GO:0042597; Cellular component:periplasmic space

Cross-references [?]

PROSITE PS51008; MULTIHEME_CYTC; 1;
Pfam PF02335; Cytochrom_C552; 1;
General Signal; -; 1;

Features [?]

From: NRFA_ECOLI (P0ABK9)
Key From To Description Tag Condition FTGroup
BINDING 94 94 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="3"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 126 126 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="1"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 K 1
BINDING 164 164 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="2"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 213 213 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="3"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 215 215 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"		 E 2
BINDING 216 216 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"		 Y 2
BINDING 261 261 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"		 K 2
BINDING 263 263 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"		 Q 2
BINDING 275 275 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="5"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 286 286 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="4"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 301 301 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="2"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 318 318 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="5"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 393 393 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="4"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"		 H 1
BINDING 122 122 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="1"
/note="covalent"		 C 3
BINDING 125 125 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="1"
/note="covalent"		 C 3
BINDING 160 160 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="2"
/note="covalent"		 C 4
BINDING 163 163 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="2"
/note="covalent"		 C 4
BINDING 209 209 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="3"
/note="covalent"		 C 5
BINDING 212 212 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="3"
/note="covalent"		 C 5
BINDING 216 216 /ligand="substrate" Y
BINDING 264 264 /ligand="substrate" H
BINDING 282 282 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="4"
/note="covalent"		 C 6
BINDING 285 285 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="4"
/note="covalent"		 C 6
BINDING 314 314 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="5"
/note="covalent"		 C 7
BINDING 317 317 /ligand="heme c"
/ligand_id="ChEBI:CHEBI:61717"
/ligand_label="5"
/note="covalent"		 C 7

Additional information [?]

Size range 463-538 amino acids
Related rules None
Fusion Nter: None Cter: None



View rule in raw text format (no links)