HAMAP rule MF_01182
General rule information
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Accession | MF_01182 |
Dates | 24-NOV-2006 (Created)
25-APR-2024 (Last updated, Version 33) |
Name | Cytochrom_C552 |
Scope(s) |
Bacteria Bacteroidota Pseudomonadota |
Template(s) | P0ABK9 (NRFA_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01182 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | NRFA |
Protein name | RecName: Full=Cytochrome c-552; EC=1.7.2.2; AltName: Full=Ammonia-forming cytochrome c nitrite reductase; Short=Cytochrome c nitrite reductase; Flags: Precursor; |
Gene name | Name=nrfA; |
Comments
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FUNCTION | Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process. |
CATALYTIC ACTIVITY | Reaction=6 Fe(III)-[cytochrome c] + 2 H2O + NH4(+) = 6 Fe(II)- [cytochrome c] + 8 H(+) + nitrite; Xref=Rhea:RHEA:13089, Rhea:RHEA- COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:28938, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.7.2.2; |
case <FTGroup:2> | |
COFACTOR | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 1 Ca(2+) ion per monomer.; |
end case | |
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7> | |
COFACTOR | Name=heme c; Xref=ChEBI:CHEBI:61717; Note=Binds 5 heme c groups covalently per monomer.; |
end case | |
PATHWAY | Nitrogen metabolism; nitrate reduction (assimilation). |
SUBCELLULAR LOCATION | Periplasm. |
SIMILARITY | Belongs to the cytochrome c-552 family. |
Keywords
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case <FTGroup:2> | |
Calcium | |
end case | |
Electron transport | |
case <FTGroup:3> or <FTGroup:4> or <FTGroup:5> or <FTGroup:6> or <FTGroup:7> | |
Heme | |
end case | |
case <FTGroup:1> | |
Iron | |
Metal-binding | |
end case | |
Oxidoreductase | |
Periplasm | |
Signal | |
Transport |
Gene Ontology
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case <FTGroup:2> | |
GO:0005509; Molecular function:calcium ion binding | |
end case | |
case <FTGroup:1> | |
GO:0005506; Molecular function:iron ion binding | |
end case | |
GO:0042279; Molecular function:nitrite reductase (cytochrome, ammonia-forming) activity | |
GO:0042128; Biological process:nitrate assimilation | |
GO:0042597; Cellular component:periplasmic space |
Cross-references
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Features
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From: NRFA_ECOLI (P0ABK9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 94 | 94 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 126 | 126 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
K | 1 | |||||||
BINDING | 164 | 164 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 213 | 213 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 215 | 215 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" |
E | 2 | |||||||
BINDING | 216 | 216 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" |
Y | 2 | |||||||
BINDING | 261 | 261 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" |
K | 2 | |||||||
BINDING | 263 | 263 | /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108" |
Q | 2 | |||||||
BINDING | 275 | 275 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 286 | 286 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 301 | 301 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 318 | 318 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 393 | 393 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
H | 1 | |||||||
BINDING | 122 | 122 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /note="covalent" |
C | 3 | |||||||
BINDING | 125 | 125 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="1" /note="covalent" |
C | 3 | |||||||
BINDING | 160 | 160 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /note="covalent" |
C | 4 | |||||||
BINDING | 163 | 163 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="2" /note="covalent" |
C | 4 | |||||||
BINDING | 209 | 209 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /note="covalent" |
C | 5 | |||||||
BINDING | 212 | 212 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="3" /note="covalent" |
C | 5 | |||||||
BINDING | 216 | 216 | /ligand="substrate" | Y | ||||||||
BINDING | 264 | 264 | /ligand="substrate" | H | ||||||||
BINDING | 282 | 282 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /note="covalent" |
C | 6 | |||||||
BINDING | 285 | 285 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="4" /note="covalent" |
C | 6 | |||||||
BINDING | 314 | 314 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /note="covalent" |
C | 7 | |||||||
BINDING | 317 | 317 | /ligand="heme c" /ligand_id="ChEBI:CHEBI:61717" /ligand_label="5" /note="covalent" |
C | 7 |
Additional information
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Size range | 463-538 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |