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HAMAP rule MF_01192

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General rule information [?]

Accession MF_01192
Dates 12-JUL-2007 (Created)
19-NOV-2022 (Last updated, Version 15)
Name Xni
Scope(s) Bacteria
Template(s) P38506 (XNI_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01192 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier XNI
Protein name RecName: Full=Flap endonuclease Xni;
Gene name Name=xni; Synonyms=ygdG;

Comments [?]

FUNCTIONHas flap endonuclease activity. During DNA replication, flap endonucleases cleave the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 Mg(2+) per subunit. Only one magnesium ion has a direct interaction with the protein, the other interactions are indirect.;
COFACTOR Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Binds 1 K(+) per subunit. The potassium ion strongly increases the affinity for DNA.;
SIMILARITYBelongs to the Xni family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0003677; Molecular function:DNA binding
GO:0017108; Molecular function:5'-flap endonuclease activity
GO:0030955; Molecular function:potassium ion binding
GO:0033567; Biological process:DNA replication, Okazaki fragment processing

Cross-references [?]

Pfam PF01367; 5_3_exonuc; 1;
Pfam PF02739; 5_3_exonuc_N; 1;

Features [?]

From: XNI_ECOLI (P38506)
Key From To Description Tag Condition FTGroup
REGION 184 189 /note="Interaction with DNA"
BINDING 104 104 /ligand="Mg(2+)"
BINDING 171 171 /ligand="K(+)"
BINDING 172 172 /ligand="K(+)"
BINDING 180 180 /ligand="K(+)"
BINDING 182 182 /ligand="K(+)"
BINDING 185 185 /ligand="K(+)"

Additional information [?]

Size range 250-290 amino acids
Related rules None
Fusion Nter: None Cter: None

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