Home  |  Contact
Annotation rule MF_01192
Send feedback

General rule information [?]

Accession MF_01192
Dates 12-JUL-2007 (Created)
19-NOV-2019 (Last updated, Version 13)
Name Xni
Scope
Bacteria; Gammaproteobacteria
Template P38506 (XNI_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
XNI
Protein name
RecName: Full=Flap endonuclease Xni;
Short=FEN;
EC 3.1.-.-;
Gene name
xni, ygdG

Comments [?]

Function Has flap endonuclease activity. During DNA replication, flap endonucleases cleave the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.
Cofactor Mg(2+)
Note: Binds 2 Mg(2+) per subunit. Only one magnesium ion has a direct interaction with the protein, the other interactions are indirect.
K(+)
Note: Binds 1 K(+) per subunit. The potassium ion strongly increases the affinity for DNA.
Similarity Belongs to the Xni family.

Keywords [?]

DNA-binding
Endonuclease
Hydrolase
Magnesium
Metal-binding
Nuclease
Potassium

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0003677; Molecular function: DNA binding.
GO:0017108; Molecular function: 5'-flap endonuclease activity.
GO:0030955; Molecular function: potassium ion binding.
GO:0033567; Biological process: DNA replication, Okazaki fragment processing.

Cross-references [?]

Pfam PF01367; 5_3_exonuc; 1; trigger=Yes;
PF02739; 5_3_exonuc_N; 1;

Features [?]

From: XNI_ECOLI (P38506)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     184     189       Interaction with DNA        
METAL     104     104       Magnesium     D  
METAL     171     171       Potassium; via carbonyl oxygen     [LIMF]  
METAL (Optional)     172     172       Potassium; via carbonyl oxygen     A  
METAL (Optional)     180     180       Potassium; via carbonyl oxygen     P  
METAL     182     182       Potassium; via carbonyl oxygen     [VI]  
METAL     185     185       Potassium; via carbonyl oxygen     [IV]  

Additional information [?]

Size range 250-290 amino acids
Related rules None
Fusion None


View rule in raw text format (no links)