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Annotation rule MF_01228
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General rule information [?]

Accession MF_01228
Dates 30-OCT-2002 (Created)
19-NOV-2019 (Last updated, Version 38)
Name Met_tRNA_synth_type2
Scope
Bacteria
Templates P23395 (SYM_THET8); P9WFU5 (SYM_MYCTU); P23920 (SYM_GEOSE): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYM
Protein name
RecName: Full=Methionine--tRNA ligase;
EC 6.1.1.10;
AltName: Full=Methionyl-tRNA synthetase;
Short=MetRS;
Gene name
metG

Comments [?]

Function Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity RHEA:13481: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
EC 6.1.1.10
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
case <Feature:PS50886>
Subunit Homodimer.
end case
case not <Feature:PS50886> and <OC:Bacteria>
Subunit Monomer.
end case
Subcellular location Cytoplasm.
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
end case
case not <FT:3>
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
end case

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
case <Feature:PS50886>
RNA-binding
tRNA-binding
end case
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR01041; TRNASYNTHMET; 1;
TIGRFAMs TIGR00398; MetG; 1;
TIGR00399; MetG_C_term; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
PS50886; TRBD; 0-1; trigger=PRU00209;

Features [?]

From: SYM_THET8 (P23395)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     12     22       'HIGH' region     [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN]  
MOTIF     297     301       'KMSKS' region     K-[MI]-[SG]-K-[ST]  
METAL     127     127       Zinc     C  
METAL     130     130       Zinc     C  
METAL     144     144       Zinc     C  
METAL     147     147       Zinc     [HC]  
case not <FT:6>
From: SYM_SYNY3 (Q55729)
METAL     152     152       Zinc     [HC]  
end case
case not <FT:7>
From: SYM_MYCGE (P47267)
METAL     147     147       Zinc     [HC]  
end case
BINDING     300     300       ATP     K  

Additional information [?]

Size range 497-683 amino acids
Related rules MF_00098 (SYM)
Fusion None
Comments Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3.


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