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HAMAP rule MF_01228

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01228
Accession MF_01228
Dates 28-FEB-2005 (Created)
03-JUL-2025 (Last updated, Version 32)
Name Met_tRNA_synth_type2
Scope(s) Bacteria
Template(s) P23395; P9WFU5; P23920; [ Recover all ]
Triggered by HAMAP; MF_01228 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYM
Protein name RecName: Full=Methionine--tRNA ligase;
                 EC=6.1.1.10;
AltName: Full=Methionyl-tRNA synthetase;
                 Short=MetRS;
Gene name Name=metG;

Comments [?]

FUNCTIONCatalyzes the attachment of L-methionine to tRNA(Met). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
CATALYTIC ACTIVITY Reaction=tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate; Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667, Rhea:RHEA- COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530, ChEBI:CHEBI:456215; EC=6.1.1.10; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13482;
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
case <Feature:PS50886>
SUBUNITHomodimer.
end case
case not <Feature:PS50886> and <OC:Bacteria>
SUBUNITMonomer.
end case
SUBCELLULAR LOCATIONCytoplasm.
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
end case
case not <FT:3>
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
end case

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
case <Feature:PS50886>
RNA-binding
tRNA-binding
end case
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004825; Molecular function:methionine-tRNA ligase activity
GO:0006431; Biological process:methionyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR01041; TRNASYNTHMET; 1;
NCBIfam TIGR00398; MetG; 1;
NCBIfam TIGR00399; MetG_C_term; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
PROSITE PS50886; TRBD; 0-1;

Features [?]

From: SYM_THET8 (P23395)
Key From To Description Tag Condition FTGroup
MOTIF 12 22 /note="'HIGH' region" [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN]
MOTIF 297 301 /note="'KMSKS' region" K-[MI]-[SG]-K-[ST]
BINDING 127 127 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C
BINDING 130 130 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C
BINDING 144 144 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C
BINDING 147 147 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 [HC]
From: SYM_SYNY3 (Q55729)
Key From To Description Tag Condition FTGroup
case not <FT:6>
BINDING 152 152 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 [HC]
end case
From: SYM_MYCGE (P47267)
Key From To Description Tag Condition FTGroup
case not <FT:7>
BINDING 147 147 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 [HC]
end case
BINDING 300 300 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 K

Additional information [?]

Size range 497-683 amino acids
Related rules MF_00098
Fusion Nter: None Cter: None
Comments Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3.



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