HAMAP rule MF_01228

General rule information [?]

Accession	MF_01228
Dates	30-OCT-2002 (Created) 1-JUN-2023 (Last updated, Version 41)

Name	Met_tRNA_synth_type2

Scope

Bacteria

Templates

P23395 (SYM_THET8); P9WFU5 (SYM_MYCTU); P23920 (SYM_GEOSE): [Recover all]

Triggered by

HAMAP; MF_01228 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SYM

Protein name

RecName:		Full=Methionine--tRNA ligase;
		EC 6.1.1.10;
AltName:		Full=Methionyl-tRNA synthetase;
		Short=MetRS;

Gene name

metG

Comments [?]

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity	RHEA:13481: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met) EC 6.1.1.10

case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)

Cofactor

Zn(2+)
Note: Binds 1 zinc ion per subunit.

end case

case <Feature:PS50886>

Subunit

Homodimer.

end case

case not <Feature:PS50886> and <OC:Bacteria>

Subunit

Monomer.

end case

Subcellular location

Cytoplasm.

case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)

Similarity

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

end case

case not <FT:3>

Similarity

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

end case

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding

case <Feature:PS50886>

RNA-binding
tRNA-binding

end case

case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)

Metal-binding
Zinc

end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00133; tRNA-synt_1; 1;
PRINTS	PR01041; TRNASYNTHMET; 1;
NCBIfam	TIGR00398; MetG; 1;
	TIGR00399; MetG_C_term; 1;
PROSITE	PS00178; AA_TRNA_LIGASE_I; 1;
	PS50886; TRBD; 0-1; trigger=PRU00209;

Features [?]

From: SYM_THET8 (P23395)

Key From To Description Tag Condition FTGroup

MOTIF 12 22 'HIGH' region [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN]

MOTIF 297 301 'KMSKS' region K-[MI]-[SG]-K-[ST]

BINDING 127 127 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 130 130 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 144 144 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 147 147 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [HC]

case not <FT:6>

From: SYM_SYNY3 (Q55729)

BINDING 152 152 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [HC]

end case

case not <FT:7>

From: SYM_MYCGE (P47267)

BINDING 147 147 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [HC]

end case

BINDING 300 300 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

Additional information [?]

Size range	497-683 amino acids
Related rules	MF_00098 (SYM)
Fusion	None
Comments	Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3.

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