HAMAP rule MF_01228
General rule information
[?]
Accession | MF_01228 |
Dates | 30-OCT-2002 (Created) 1-JUN-2023 (Last updated, Version 41) |
Name | Met_tRNA_synth_type2 |
Scope | Bacteria |
Templates | P23395 (SYM_THET8); P9WFU5 (SYM_MYCTU); P23920 (SYM_GEOSE): [Recover all] |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Gene name |
|
Comments
[?]
Function | Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. |
Catalytic activity | RHEA:13481: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
EC 6.1.1.10 |
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
end case
case <Feature:PS50886>
Subunit | Homodimer. |
end case
case not <Feature:PS50886> and <OC:Bacteria>
Subunit | Monomer. |
end case
Subcellular location | Cytoplasm. |
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. |
end case
case not <FT:3>
Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. |
end case
Keywords
[?]
case <Feature:PS50886>
end case
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
end case
Gene Ontology
[?]
GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
[?]
Pfam | PF00133; tRNA-synt_1; 1; |
PRINTS | PR01041; TRNASYNTHMET; 1; |
NCBIfam | TIGR00398; MetG; 1; |
TIGR00399; MetG_C_term; 1; | |
PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
PS50886; TRBD; 0-1; trigger=PRU00209; |
Features
[?]
From: SYM_THET8 (P23395) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF | 12 | 22 | 'HIGH' region | [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN] | ||||||||
MOTIF | 297 | 301 | 'KMSKS' region | K-[MI]-[SG]-K-[ST] | ||||||||
BINDING | 127 | 127 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 130 | 130 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 144 | 144 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
BINDING | 147 | 147 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [HC] |
case not <FT:6>
From: SYM_SYNY3 (Q55729) | ||||||||||||
BINDING | 152 | 152 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [HC] |
end case
case not <FT:7>
From: SYM_MYCGE (P47267) | ||||||||||||
BINDING | 147 | 147 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | [HC] |
end case
Additional information
[?]
Size range | 497-683 amino acids |
Related rules | MF_00098 (SYM) |
Fusion | None |
Comments | Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3. |