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HAMAP rule MF_01237

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General rule information [?]

Accession MF_01237
Dates 21-MAR-2003 (Created)
12-MAR-2024 (Last updated, Version 37)
Name N_acetylneuram_lyase
Scope(s) Bacteria
Template(s) P0A6L4 (NANA_ECOLI); P44539 (NANA_HAEIN); Q9S4K9 (NANA_CLOPE); Q2G160 (NANA_STAA8); P59407 (NANA_LACPL); Q6GK01 (NANA_STAAR); A7B555 (NANA_MEDG7); Q8RDN6 (NANA_FUSNN); Q9CKB0 (NANA_PASMU); [ Recover all ]
Triggered by HAMAP; MF_01237 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NANA
Protein name RecName: Full=N-acetylneuraminate lyase;
                 Short=Neu5Ac lyase;
                 Short=NAL;
                 EC=4.1.3.3;
AltName: Full=N-acetylneuraminate pyruvate-lyase;
AltName: Full=N-acetylneuraminic acid aldolase;
AltName: Full=Sialate lyase;
AltName: Full=Sialic acid aldolase;
AltName: Full=Sialic acid lyase;
Gene name Name=nanA;

Comments [?]

FUNCTIONCatalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
CATALYTIC ACTIVITY Reaction=aceneuramate = N-acetyl-D-mannosamine + pyruvate; Xref=Rhea:RHEA:23296, ChEBI:CHEBI:15361, ChEBI:CHEBI:17122, ChEBI:CHEBI:173083; EC=4.1.3.3;
PATHWAYAmino-sugar metabolism; N-acetylneuraminate degradation; D- fructose 6-phosphate from N-acetylneuraminate: step 1/5.
SUBUNITHomotetramer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the DapA family. NanA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0008747; Molecular function:N-acetylneuraminate lyase activity
GO:0005975; Biological process:carbohydrate metabolic process
GO:0019262; Biological process:N-acetylneuraminate catabolic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00701; DHDPS; 1;
PRINTS PR00146; DHPICSNTHASE; 1;
NCBIfam TIGR00683; nanA; 1;
PROSITE PS00665; DHDPS_1; 1;
PROSITE PS00666; DHDPS_2; 1;
PIRSF PIRSF001365; DHDPS; 1;

Features [?]

From: NANA_ECOLI (P0A6L4)
Key From To Description Tag Condition FTGroup
ACT_SITE 137 137 /note="Proton donor" Y
ACT_SITE 165 165 /note="Schiff-base intermediate with substrate" K
BINDING 47 47 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
S
BINDING 48 48 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
[TS]
BINDING 167 167 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
[TS]
BINDING 189 189 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
G
BINDING 191 191 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
D
BINDING 192 192 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
E
BINDING 208 208 /ligand="aceneuramate"
/ligand_id="ChEBI:CHEBI:173083"
[SG]

Additional information [?]

Size range 280-320 amino acids
Related rules MF_00418
MF_00694
Fusion Nter: None Cter: None



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