HAMAP rule MF_01237

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01237
Accession	MF_01237
Dates	28-FEB-2005 (Created) 02-MAY-2024 (Last updated, Version 31)
Name	N_acetylneuram_lyase
Scope(s)	Bacteria
Template(s)	P0A6L4; P44539; Q9S4K9; Q2G160; P59407; Q6GK01; A7B555; Q8RDN6; Q9CKB0; [ Recover all ]
Triggered by	HAMAP; MF_01237 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	NANA
Protein name	RecName: Full=N-acetylneuraminate lyase; Short=Neu5Ac lyase; Short=NAL; EC=4.1.3.3; AltName: Full=N-acetylneuraminate pyruvate-lyase; AltName: Full=N-acetylneuraminic acid aldolase; AltName: Full=Sialate lyase; AltName: Full=Sialic acid aldolase; AltName: Full=Sialic acid lyase;
Gene name	Name=nanA;

Comments [?]

FUNCTION	Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
CATALYTIC ACTIVITY	Reaction=aceneuramate = aldehydo-N-acetyl-D-mannosamine + pyruvate; Xref=Rhea:RHEA:23296, ChEBI:CHEBI:15361, ChEBI:CHEBI:17122, ChEBI:CHEBI:173083; EC=4.1.3.3;
PATHWAY	Amino-sugar metabolism; N-acetylneuraminate degradation; D- fructose 6-phosphate from N-acetylneuraminate: step 1/5.
SUBUNIT	Homotetramer.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the DapA family. NanA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0008747; Molecular function:N-acetylneuraminate lyase activity

GO:0005975; Biological process:carbohydrate metabolic process

GO:0019262; Biological process:N-acetylneuraminate catabolic process

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: NANA_ECOLI (P0A6L4)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

137

/note="Proton donor"

ACT_SITE

165

/note="Schiff-base intermediate with substrate"

BINDING