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Annotation rule MF_01252
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General rule information [?]

Accession MF_01252
Dates 24-AUG-2004 (Created)
19-NOV-2019 (Last updated, Version 29)
Name Hmp
Scope
Bacteria
Templates P24232 (HMP_ECOLI); P39662 (HMP_CUPNH); P26353 (HMP_SALTY): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HMP
Protein name
RecName: Full=Flavohemoprotein;
AltName: Full=Flavohemoglobin;
AltName: Full=Hemoglobin-like protein;
AltName: Full=Nitric oxide dioxygenase;
Short=NO oxygenase;
Short=NOD;
EC 1.14.12.17;
Gene name
hmp

Comments [?]

case <OC:Proteobacteria> or <OC:Bacillales>
Function Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.
end case
Catalytic activity RHEA:19465: NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate
EC 1.14.12.17
RHEA:19469: NADH + 2 nitric oxide + 2 O2 = H(+) + NAD(+) + 2 nitrate
EC 1.14.12.17
Cofactor heme b
Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
FAD
Note: Binds 1 FAD per subunit.
Domain Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.
Similarity Belongs to the globin family. Two-domain flavohemoproteins subfamily.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords [?]

case <OC:Proteobacteria> or <OC:Bacillales>
end case

Gene Ontology [?]

GO:0005344; Molecular function: oxygen carrier activity.
GO:0008941; Molecular function: nitric oxide dioxygenase activity.
GO:0015671; Biological process: oxygen transport.

Cross-references [?]

Pfam PF00970; FAD_binding_6; 1;
PF00042; Globin; 1;
PF00175; NAD_binding_1; 1;
PRINTS PR00371; FPNCR; 1;
PR00410; PHEHYDRXLASE; 1;
PR00188; PLANTGLOBIN; 1;
PR00406; CYTB5RDTASE; 1;
PR00409; PHDIOXRDTASE; 1;
PROSITE PS01033; GLOBIN; 1; trigger=PRU00238;
PS51384; FAD_FR; 1; trigger=PRU00716;

Features [?]

From: HMP_ECOLI (P24232)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     151     251       FAD-binding        
NP_BIND     204     207       FAD     R-[QN]-Y-S  
NP_BIND     268     273       NADP     G-[VI]-G-[QILAV]-T-P  
NP_BIND     389     392       FAD     [CLFVT]-F-G-[PST]  
REGION     147     Cter       Reductase        
REGION     259     Cter       NAD or NADP-binding        
ACT_SITE     95     95       Charge relay system     Y  
ACT_SITE     135     135       Charge relay system     E  
METAL     85     85       Iron (heme proximal ligand)     H  
BINDING     188     188       FAD     Y  
SITE     29     29       Involved in heme-bound ligand stabilization and O-O bond activation     Y  
SITE     84     84       Influences the redox potential of the prosthetic heme and FAD groups     K  
SITE     388     388       Influences the redox potential of the prosthetic heme and FAD groups     E  

Additional information [?]

Size range 392-411 amino acids
Related rules None
Fusion None