HAMAP rule MF_01252

General rule information [?]

Accession	MF_01252
Dates	24-AUG-2004 (Created) 26-JAN-2023 (Last updated, Version 34)

Name

Hmp

Scope

Bacteria

Templates

P24232 (HMP_ECOLI); P39662 (HMP_CUPNH); P26353 (HMP_SALTY): [Recover all]

Triggered by

HAMAP; MF_01252 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

HMP

Protein name

RecName:		Full=Flavohemoprotein;
AltName:		Full=Flavohemoglobin;
AltName:		Full=Hemoglobin-like protein;
AltName:		Full=Nitric oxide dioxygenase;
		Short=NO oxygenase;
		Short=NOD;
		EC 1.14.12.17;

Gene name

hmp

Comments [?]

case <OC:Pseudomonadota> or <OC:Bacillales>

Function

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.

end case

Catalytic activity	RHEA:19465: NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate EC 1.14.12.17
	RHEA:19469: NADH + 2 nitric oxide + 2 O2 = H(+) + NAD(+) + 2 nitrate EC 1.14.12.17
Cofactor	heme b Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
	FAD Note: Binds 1 FAD per subunit.
Domain	Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.
Similarity	Belongs to the globin family. Two-domain flavohemoproteins subfamily.
	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords [?]

case <OC:Pseudomonadota> or <OC:Bacillales>

Detoxification

end case

FAD
Flavoprotein
Heme
Iron
Metal-binding
NAD
NADP
Oxidoreductase
Oxygen transport
Transport

Gene Ontology [?]

GO:0005344; Molecular function: oxygen carrier activity.
GO:0008941; Molecular function: nitric oxide dioxygenase activity.
GO:0015671; Biological process: oxygen transport.

Cross-references [?]

Pfam	PF00970; FAD_binding_6; 1;
	PF00042; Globin; 1;
	PF00175; NAD_binding_1; 1;
PRINTS	PR00371; FPNCR; 1;
	PR00410; PHEHYDRXLASE; 1;
	PR00188; PLANTGLOBIN; 1;
	PR00406; CYTB5RDTASE; 1;
	PR00409; PHDIOXRDTASE; 1;
PROSITE	PS01033; GLOBIN; 1; trigger=PRU00238;
	PS51384; FAD_FR; 1; trigger=PRU00716;

Features [?]

From: HMP_ECOLI (P24232)

Key From To Description Tag Condition FTGroup

DOMAIN 151 251 FAD-binding

BINDING 204 207 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 R-[QN]-Y-S

BINDING 268 273 /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349 G-[VI]-G-[QILAV]-T-P

BINDING 389 392 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 [CLFVT]-F-G-[PST]

REGION 147 Cter Reductase

ACT_SITE 95 95 Charge relay system Y

ACT_SITE 135 135 Charge relay system E

BINDING 85 85 /ligand="heme b" /ligand_id="ChEBI:CHEBI:60344" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="proximal binding residue H

BINDING 188 188 /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692 Y

SITE 29 29 Involved in heme-bound ligand stabilization and O-O bond activation Y

SITE 84 84 Influences the redox potential of the prosthetic heme and FAD groups K

SITE 388 388 Influences the redox potential of the prosthetic heme and FAD groups E

Additional information [?]

Size range	392-411 amino acids
Related rules	None
Fusion	None

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