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Annotation rule MF_01253 |
General rule information
[?]
Accession |
MF_01253 |
Dates |
9-DEC-2004 (Created) 14-MAY-2022 (Last updated, Version 29) |
Scope |
Bacteria; Enterobacterales |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Endonuclease 8;
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AltName:
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Full=DNA glycosylase/AP lyase Nei;
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EC 3.2.2.-;
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EC 4.2.99.18;
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AltName:
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Full=DNA-(apurinic or apyrimidinic site) lyase Nei;
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AltName:
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Full=Endonuclease VIII;
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Function |
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
Catalytic activity |
RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)
EC 4.2.99.18
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Cofactor |
Zn(2+) Note: Binds 1 zinc ion per subunit. |
Similarity |
Belongs to the FPG family. |
case <Feature:PS51066>
end case
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000703; Molecular function: oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity.
case <Feature:PS51066>
end case
From: END8_ECOLI (P50465) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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INIT_MET
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Nter
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Nter
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Removed
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M
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ACT_SITE
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2
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2
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Schiff-base intermediate with DNA
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P
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ACT_SITE
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3
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3
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Proton donor
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E
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ACT_SITE
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53
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53
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Proton donor; for beta-elimination activity
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K
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ACT_SITE
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253
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253
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Proton donor; for delta-elimination activity
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R
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BINDING
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70
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70
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/ligand="DNA" /ligand_id="16991
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Q
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BINDING
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125
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125
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/ligand="DNA" /ligand_id="16991
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R
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BINDING
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169
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169
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/ligand="DNA" /ligand_id="16991
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N
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Additional information
[?]
Size range |
260-265 amino acids |
Related rules |
None |
Fusion |
None |