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HAMAP rule MF_01253

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General rule information [?]

Accession MF_01253
Dates 9-DEC-2004 (Created)
30-JAN-2024 (Last updated, Version 31)
Name Endonuclease_8
Scope(s) Bacteria
Template(s) P50465 (END8_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01253 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier END8
Protein name RecName: Full=Endonuclease 8;
AltName: Full=DNA glycosylase/AP lyase Nei;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase Nei;
AltName: Full=Endonuclease VIII;
Gene name Name=nei;

Comments [?]

FUNCTIONInvolved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
CATALYTIC ACTIVITY Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
SIMILARITYBelongs to the FPG family.

Keywords [?]

Gene Ontology [?]

GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity
GO:0000703; Molecular function:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity
case <Feature:PS51066>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF01149; Fapy_DNA_glyco; 1;
Pfam PF06831; H2TH; 1;
Pfam PF06827; zf-FPG_IleRS; 1;
PROSITE PS01242; ZF_FPG_1; 1;
PROSITE PS51066; ZF_FPG_2; 1;

Features [?]

From: END8_ECOLI (P50465)
Key From To Description Tag Condition FTGroup
INIT_MET Nter Nter /note="Removed" M
ACT_SITE 2 2 /note="Schiff-base intermediate with DNA" P
ACT_SITE 3 3 /note="Proton donor" E
ACT_SITE 53 53 /note="Proton donor; for beta-elimination activity" K
ACT_SITE 253 253 /note="Proton donor; for delta-elimination activity" R
BINDING 70 70 /ligand="DNA"
BINDING 125 125 /ligand="DNA"
BINDING 169 169 /ligand="DNA"

Additional information [?]

Size range 260-265 amino acids
Related rules None
Fusion Nter: None Cter: None

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