HAMAP rule MF_01253
General rule information
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| Accession | MF_01253 |
| Dates | 9-DEC-2004 (Created)
19-NOV-2022 (Last updated, Version 30) |
| Name | Endonuclease_8 |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P50465 (END8_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01253 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | END8 |
| Protein name | RecName: Full=Endonuclease 8; AltName: Full=DNA glycosylase/AP lyase Nei; EC=3.2.2.-; EC=4.2.99.18; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase Nei; AltName: Full=Endonuclease VIII; |
| Gene name | Name=nei; |
Comments
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| FUNCTION | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
| CATALYTIC ACTIVITY | Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho- 2'-deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| SIMILARITY | Belongs to the FPG family. |
Keywords
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| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Glycosidase | |
| Hydrolase | |
| Lyase | |
| Multifunctional enzyme | |
| case <Feature:PS51066> | |
| Metal-binding | |
| Zinc | |
| Zinc-finger | |
| end case | |
Gene Ontology
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| GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity | |
| GO:0000703; Molecular function:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity | |
| case <Feature:PS51066> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006281; Biological process:DNA repair | |
Cross-references
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| Pfam | PF01149; Fapy_DNA_glyco; 1; |
| Pfam | PF06831; H2TH; 1; |
| Pfam | PF06827; zf-FPG_IleRS; 1; |
| PROSITE | PS51068; FPG_CAT; 1; |
| PROSITE | PS01242; ZF_FPG_1; 1; |
| PROSITE | PS51066; ZF_FPG_2; 1; |
Features
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| From: END8_ECOLI (P50465) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| INIT_MET | Nter | Nter | /note="Removed" | M | ||||||||
| ACT_SITE | 2 | 2 | /note="Schiff-base intermediate with DNA" | P | ||||||||
| ACT_SITE | 3 | 3 | /note="Proton donor" | E | ||||||||
| ACT_SITE | 53 | 53 | /note="Proton donor; for beta-elimination activity" | K | ||||||||
| ACT_SITE | 253 | 253 | /note="Proton donor; for delta-elimination activity" | R | ||||||||
| BINDING | 70 | 70 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
Q | ||||||||
| BINDING | 125 | 125 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
R | ||||||||
| BINDING | 169 | 169 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991" |
N | ||||||||
Additional information
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| Size range | 260-265 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |