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HAMAP rule MF_01253

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General rule information [?]

Accession MF_01253
Dates 9-DEC-2004 (Created)
19-NOV-2022 (Last updated, Version 30)
Name Endonuclease_8
Bacteria; Enterobacterales
Template P50465 (END8_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Endonuclease 8;
AltName: Full=DNA glycosylase/AP lyase Nei;
EC 3.2.2.-;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase Nei;
AltName: Full=Endonuclease VIII;
Gene name

Comments [?]

Function Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Catalytic activity RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
Similarity Belongs to the FPG family.

Keywords [?]

case <Feature:PS51066>
end case

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000703; Molecular function: oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity.
case <Feature:PS51066>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF01149; Fapy_DNA_glyco; 1;
PF06831; H2TH; 1;
PF06827; zf-FPG_IleRS; 1;
PS01242; ZF_FPG_1; 1;
PS51066; ZF_FPG_2; 1; trigger=PRU00391;

Features [?]

From: END8_ECOLI (P50465)
Key     From     To       Description   Tag   Condition   FTGroup
INIT_MET     Nter     Nter       Removed     M  
ACT_SITE     2     2       Schiff-base intermediate with DNA     P  
ACT_SITE     3     3       Proton donor     E  
ACT_SITE     53     53       Proton donor; for beta-elimination activity     K  
ACT_SITE     253     253       Proton donor; for delta-elimination activity     R  
BINDING     70     70       /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991     Q  
BINDING     125     125       /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991     R  
BINDING     169     169       /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991     N  

Additional information [?]

Size range 260-265 amino acids
Related rules None
Fusion None