HAMAP rule MF_01253

General rule information [?]

Accession	MF_01253
Dates	9-DEC-2004 (Created) 19-NOV-2022 (Last updated, Version 30)

Name	Endonuclease_8

Scope

Bacteria; Enterobacterales

Template

P50465 (END8_ECOLI)

Triggered by

HAMAP; MF_01253 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

END8

Protein name

RecName:		Full=Endonuclease 8;
AltName:		Full=DNA glycosylase/AP lyase Nei;
		EC 3.2.2.-;
		EC 4.2.99.18;
AltName:		Full=DNA-(apurinic or apyrimidinic site) lyase Nei;
AltName:		Full=Endonuclease VIII;

Gene name

nei

Comments [?]

Function	Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Catalytic activity	RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+) EC 4.2.99.18
Cofactor	Zn(2+) Note: Binds 1 zinc ion per subunit.
Similarity	Belongs to the FPG family.

Keywords [?]

DNA damage
DNA repair
DNA-binding
Glycosidase
Hydrolase
Lyase
Multifunctional enzyme

case <Feature:PS51066>

Metal-binding
Zinc
Zinc-finger

end case

Gene Ontology [?]

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000703; Molecular function: oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity.

case <Feature:PS51066>

GO:0008270; Molecular function: zinc ion binding.

end case

GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam	PF01149; Fapy_DNA_glyco; 1;
	PF06831; H2TH; 1;
	PF06827; zf-FPG_IleRS; 1;
PROSITE	PS51068; FPG_CAT; 1;
	PS01242; ZF_FPG_1; 1;
	PS51066; ZF_FPG_2; 1; trigger=PRU00391;

Features [?]

From: END8_ECOLI (P50465)

Key From To Description Tag Condition FTGroup

INIT_MET Nter Nter Removed M

ACT_SITE 2 2 Schiff-base intermediate with DNA P

ACT_SITE 3 3 Proton donor E

ACT_SITE 53 53 Proton donor; for beta-elimination activity K

ACT_SITE 253 253 Proton donor; for delta-elimination activity R

BINDING 70 70 /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 Q

BINDING 125 125 /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 R

BINDING 169 169 /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 N

Additional information [?]

Size range	260-265 amino acids
Related rules	None
Fusion	None

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