HAMAP rule MF_01253
General rule information
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Accession | MF_01253 |
Dates | 9-DEC-2004 (Created) 19-NOV-2022 (Last updated, Version 30) |
Name | Endonuclease_8 |
Scope | Bacteria; Enterobacterales |
Template | P50465 (END8_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
Catalytic activity | RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)
EC 4.2.99.18 |
Cofactor | Zn(2+) Note: Binds 1 zinc ion per subunit. |
Similarity | Belongs to the FPG family. |
Keywords
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case <Feature:PS51066>
end case
Gene Ontology
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GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0000703; Molecular function: oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity.
GO:0000703; Molecular function: oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity.
case <Feature:PS51066>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.
Cross-references
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Pfam | PF01149; Fapy_DNA_glyco; 1; |
PF06831; H2TH; 1; | |
PF06827; zf-FPG_IleRS; 1; | |
PROSITE | PS51068; FPG_CAT; 1; |
PS01242; ZF_FPG_1; 1; | |
PS51066; ZF_FPG_2; 1; trigger=PRU00391; |
Features
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From: END8_ECOLI (P50465) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
INIT_MET | Nter | Nter | Removed | M | ||||||||
ACT_SITE | 2 | 2 | Schiff-base intermediate with DNA | P | ||||||||
ACT_SITE | 3 | 3 | Proton donor | E | ||||||||
ACT_SITE | 53 | 53 | Proton donor; for beta-elimination activity | K | ||||||||
ACT_SITE | 253 | 253 | Proton donor; for delta-elimination activity | R | ||||||||
BINDING | 70 | 70 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 | Q | ||||||||
BINDING | 125 | 125 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 | R | ||||||||
BINDING | 169 | 169 | /ligand="DNA" /ligand_id="ChEBI:CHEBI:16991 | N |
Additional information
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Size range | 260-265 amino acids |
Related rules | None |
Fusion | None |