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HAMAP rule MF_01257

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General rule information [?]

Accession MF_01257
Dates 21-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 20)
Name CofD
Scope(s) Bacteria
Template(s) P9WP81 (FBIA_MYCTU); Q8PVT6 (COFD_METMA); Q58653 (COFD_METJA); [ Recover all ]
Triggered by HAMAP; MF_01257 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Actinomycetota>
Identifier FBIA
Protein name RecName: Full=Phosphoenolpyruvate transferase;
AltName: Full=EPPG:FO PEP transferase;
Gene name Name=fbiA;
else case <OC:Archaea>
Identifier COFD
Protein name RecName: Full=2-phospho-L-lactate transferase;
AltName: Full=EPPG:FO PEP transferase;
Gene name Name=cofD;
end case

Comments [?]

case <OC:Actinomycetota>
FUNCTIONCatalyzes the transfer of the phosphoenolpyruvate moiety from enoylpyruvoyl-2-diphospho-5'-guanosine (EPPG) to 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO) with the formation of dehydro coenzyme F420-0 and GMP.
CATALYTIC ACTIVITY Reaction=7,8-didemethyl-8-hydroxy-5-deazariboflavin + enolpyruvoyl-2- diphospho-5'-guanosine = dehydro coenzyme F420-0 + GMP + H(+); Xref=Rhea:RHEA:27510, ChEBI:CHEBI:15378, ChEBI:CHEBI:58115, ChEBI:CHEBI:59904, ChEBI:CHEBI:143701, ChEBI:CHEBI:143705; EC=;
else case <OC:Archaea>
FUNCTIONCatalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5- deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.
CATALYTIC ACTIVITY Reaction=(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8- hydroxy-5-deazariboflavin = GMP + H(+) + oxidized coenzyme F420-0; Xref=Rhea:RHEA:63444, ChEBI:CHEBI:15378, ChEBI:CHEBI:58115, ChEBI:CHEBI:59435, ChEBI:CHEBI:59904, ChEBI:CHEBI:59907; EC=;
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCofactor biosynthesis; coenzyme F420 biosynthesis.
SIMILARITYBelongs to the CofD family.

Keywords [?]

Gene Ontology [?]

GO:0016773; Molecular function:phosphotransferase activity, alcohol group as acceptor
GO:0052645; Biological process:F420-0 metabolic process

Cross-references [?]

Pfam PF01933; UPF0052; 1;
NCBIfam TIGR01819; F420_cofD; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 48 48 /ligand="7,8-didemethyl-8-hydroxy-5-deazariboflavin"
BINDING 87 87 /ligand="7,8-didemethyl-8-hydroxy-5-deazariboflavin"

Additional information [?]

Size range 296-337 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments CofD homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus, as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida. The reaction was shown to be different according to the species, but IUBMB has created a single EC number (they consider it is the same enzyme).

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