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HAMAP rule MF_01272

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General rule information [?]

Accession MF_01272
Dates 6-NOV-2006 (Created)
13-JAN-2023 (Last updated, Version 23)
Name Heme_degrading_monooxygenase
Scope
Bacteria; Bacillota
Templates Q8NX62 (HDOX1_STAAW); Q2FZE2 (HDOX1_STAA8); Q2G1J2 (HDOX2_STAA8); Q99X56 (HDOX2_STAAM); Q81L50 (HDOX_BACAN): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HDOX
case <OC:Staphylococcus>
Protein name
RecName: Full=Heme oxygenase (staphylobilin-producing);
EC 1.14.99.48;
AltName: Full=Heme-degrading monooxygenase;
AltName: Full=Iron-regulated surface determinant;
AltName: Full=Iron-responsive surface determinant;
else
Protein name
RecName: Full=Heme-degrading monooxygenase;
EC 1.14.14.18;
AltName: Full=Heme oxygenase;
AltName: Full=Iron-regulated surface determinant;
AltName: Full=Iron-responsive surface determinant;
Gene name
isdG
end case

Comments [?]

case <OC:Staphylococcus>
Function Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
Catalytic activity RHEA:37039: 5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + delta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O
EC 1.14.99.48
RHEA:37363: 5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + beta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O
EC 1.14.99.48
else
Function Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
Catalytic activity RHEA:21764: heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]
EC 1.14.14.18
end case
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Keywords [?]

Cytoplasm
Heme
Iron
Metal-binding
Monooxygenase
Oxidoreductase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0004392; Molecular function: heme oxygenase (decyclizing) activity.
GO:0016705; Molecular function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
GO:0020037; Molecular function: heme binding.
GO:0005737; Cellular component: cytoplasm.
GO:0042167; Biological process: heme catabolic process.

Cross-references [?]

PROSITE PS51725; ABM; 1; trigger=PRU01062;
Pfam PF03992; ABM; 1;

Features [?]

From: HDOX2_STAAN (Q7A827)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     21     28       /ligand="heme" /ligand_id="ChEBI:CHEBI:30413     R-F-Y-x-R-x-G-I  
BINDING     6     6       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     N  
BINDING     76     76       /ligand="heme" /ligand_id="ChEBI:CHEBI:30413" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue     H  
SITE     66     66       Transition state stabilizer        

Additional information [?]

Size range 100-121 amino acids
Related rules None
Fusion None
Comments Most Staphylococcus aureus strains have two family members; the second one is called isdI.


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