HAMAP rule MF_01272
General rule information
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Accession | MF_01272 |
Dates | 6-NOV-2006 (Created) 13-JAN-2023 (Last updated, Version 23) |
Name | Heme_degrading_monooxygenase |
Scope | Bacteria; Bacillota |
Templates | Q8NX62 (HDOX1_STAAW); Q2FZE2 (HDOX1_STAA8); Q2G1J2 (HDOX2_STAA8); Q99X56 (HDOX2_STAAM); Q81L50 (HDOX_BACAN): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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case <OC:Staphylococcus>
Protein name |
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else
Protein name |
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Gene name |
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end case
Comments
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case <OC:Staphylococcus>
Function | Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. |
Catalytic activity | RHEA:37039: 5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + delta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O
EC 1.14.99.48 |
RHEA:37363: 5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + beta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O
EC 1.14.99.48 |
else
Function | Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron. |
Catalytic activity | RHEA:21764: heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]
EC 1.14.14.18 |
end case
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily. |
Keywords
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Gene Ontology
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GO:0005506; Molecular function: iron ion binding.
GO:0004392; Molecular function: heme oxygenase (decyclizing) activity.
GO:0016705; Molecular function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
GO:0020037; Molecular function: heme binding.
GO:0005737; Cellular component: cytoplasm.
GO:0042167; Biological process: heme catabolic process.
GO:0004392; Molecular function: heme oxygenase (decyclizing) activity.
GO:0016705; Molecular function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
GO:0020037; Molecular function: heme binding.
GO:0005737; Cellular component: cytoplasm.
GO:0042167; Biological process: heme catabolic process.
Cross-references
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Features
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From: HDOX2_STAAN (Q7A827) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 21 | 28 | /ligand="heme" /ligand_id="ChEBI:CHEBI:30413 | R-F-Y-x-R-x-G-I | ||||||||
BINDING | 6 | 6 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | N | ||||||||
BINDING | 76 | 76 | /ligand="heme" /ligand_id="ChEBI:CHEBI:30413" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue | H | ||||||||
SITE | 66 | 66 | Transition state stabilizer |
Additional information
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Size range | 100-121 amino acids |
Related rules | None |
Fusion | None |
Comments | Most Staphylococcus aureus strains have two family members; the second one is called isdI. |