HAMAP rule MF_01276
General rule information
[?]
| Accession | MF_01276 |
| Dates | 29-NOV-2006 (Created)
13-OCT-2023 (Last updated, Version 23) |
| Name | Putres_aminotrans_3 |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P42588 (PAT_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01276 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | PAT |
| Protein name | RecName: Full=Putrescine aminotransferase; Short=PAT; Short=PATase; EC=2.6.1.82; AltName: Full=Cadaverine transaminase; AltName: Full=Diamine transaminase; EC=2.6.1.29; AltName: Full=Putrescine transaminase; AltName: Full=Putrescine--2-oxoglutaric acid transaminase; |
| Gene name | Name=patA; |
Comments
[?]
| FUNCTION | Catalyzes the aminotransferase reaction from putrescine to 2- oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate (gamma-aminobutyrate or GABA) via 4- aminobutanal. Also functions as a cadaverine transaminase in a a L- lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate. |
| CATALYTIC ACTIVITY | Reaction=2-oxoglutarate + an alkane-alpha,omega-diamine = an omega- aminoaldehyde + L-glutamate; Xref=Rhea:RHEA:18217, Rhea:RHEA- COMP:9766, Rhea:RHEA-COMP:12750, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:70977, ChEBI:CHEBI:133427; EC=2.6.1.29; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18218; |
| CATALYTIC ACTIVITY | Reaction=2-oxoglutarate + putrescine = 1-pyrroline + H2O + L-glutamate; Xref=Rhea:RHEA:12268, ChEBI:CHEBI:15377, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:36781, ChEBI:CHEBI:326268; EC=2.6.1.82; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12269; |
| CATALYTIC ACTIVITY | Reaction=2-oxoglutarate + cadaverine = 5-aminopentanal + L-glutamate; Xref=Rhea:RHEA:61624, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:58384, ChEBI:CHEBI:144896; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61625; |
| COFACTOR | Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; |
| PATHWAY | Amine and polyamine degradation; putrescine degradation; 4- aminobutanal from putrescine (transaminase route): step 1/1. |
| PATHWAY | Amino-acid degradation. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily. |
Keywords
[?]
Gene Ontology
[?]
| GO:0033094; Molecular function:putrescine--2-oxoglutarate transaminase activity |
| GO:0030170; Molecular function:pyridoxal phosphate binding |
| GO:0009447; Biological process:putrescine catabolic process |
| GO:0019477; Biological process:L-lysine catabolic process |
Cross-references
[?]
| PROSITE | PS00600; AA_TRANSFER_CLASS_3; 1; |
| Pfam | PF00202; Aminotran_3; 1; |
| NCBIfam | TIGR03372; putres_am_tran; 1; |
| PIRSF | PIRSF000521; Transaminase_4ab_Lys_Orn; 1; |
Features
[?]
| From: PAT_ECOLI (P42588) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 150 | 151 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
G-T | ||||||||
| BINDING | 274 | 274 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
Q | ||||||||
| BINDING | 332 | 332 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" |
T | ||||||||
| MOD_RES | 300 | 300 | /note="N6-(pyridoxal phosphate)lysine" | K | ||||||||
Additional information
[?]
| Size range | 459-468 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |