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HAMAP rule MF_01276

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General rule information [?]

Accession MF_01276
Dates 29-NOV-2006 (Created)
13-OCT-2023 (Last updated, Version 23)
Name Putres_aminotrans_3
Scope(s) Bacteria
Template(s) P42588 (PAT_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01276 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PAT
Protein name RecName: Full=Putrescine aminotransferase;
AltName: Full=Cadaverine transaminase;
AltName: Full=Diamine transaminase;
AltName: Full=Putrescine transaminase;
AltName: Full=Putrescine--2-oxoglutaric acid transaminase;
Gene name Name=patA;

Comments [?]

FUNCTIONCatalyzes the aminotransferase reaction from putrescine to 2- oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate (gamma-aminobutyrate or GABA) via 4- aminobutanal. Also functions as a cadaverine transaminase in a a L- lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate.
CATALYTIC ACTIVITY Reaction=2-oxoglutarate + an alkane-alpha,omega-diamine = an omega- aminoaldehyde + L-glutamate; Xref=Rhea:RHEA:18217, Rhea:RHEA- COMP:9766, Rhea:RHEA-COMP:12750, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:70977, ChEBI:CHEBI:133427; EC=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:18218;
CATALYTIC ACTIVITY Reaction=2-oxoglutarate + putrescine = 1-pyrroline + H2O + L-glutamate; Xref=Rhea:RHEA:12268, ChEBI:CHEBI:15377, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:36781, ChEBI:CHEBI:326268; EC=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12269;
CATALYTIC ACTIVITY Reaction=2-oxoglutarate + cadaverine = 5-aminopentanal + L-glutamate; Xref=Rhea:RHEA:61624, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:58384, ChEBI:CHEBI:144896; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61625;
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
PATHWAYAmine and polyamine degradation; putrescine degradation; 4- aminobutanal from putrescine (transaminase route): step 1/1.
PATHWAYAmino-acid degradation.
SIMILARITYBelongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily.

Keywords [?]

Gene Ontology [?]

GO:0033094; Molecular function:putrescine--2-oxoglutarate transaminase activity
GO:0030170; Molecular function:pyridoxal phosphate binding
GO:0009447; Biological process:putrescine catabolic process
GO:0019477; Biological process:L-lysine catabolic process

Cross-references [?]

Pfam PF00202; Aminotran_3; 1;
NCBIfam TIGR03372; putres_am_tran; 1;
PIRSF PIRSF000521; Transaminase_4ab_Lys_Orn; 1;

Features [?]

From: PAT_ECOLI (P42588)
Key From To Description Tag Condition FTGroup
BINDING 150 151 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 274 274 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 332 332 /ligand="pyridoxal 5'-phosphate"
/ligand_note="ligand shared between dimeric partners"
MOD_RES 300 300 /note="N6-(pyridoxal phosphate)lysine" K

Additional information [?]

Size range 459-468 amino acids
Related rules None
Fusion Nter: None Cter: None

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