HAMAP rule MF_01276

General rule information [?]

Accession	MF_01276
Dates	29-NOV-2006 (Created) 1-JUN-2023 (Last updated, Version 22)

Name	Putres_aminotrans_3

Scope

Bacteria; Enterobacterales

Template

P42588 (PAT_ECOLI)

Triggered by

HAMAP; MF_01276 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PAT

Protein name

RecName:		Full=Putrescine aminotransferase;
		Short=PAT;
		Short=PATase;
		EC 2.6.1.82;
AltName:		Full=Cadaverine transaminase;
AltName:		Full=Diamine transaminase;
		EC 2.6.1.29;
AltName:		Full=Putrescine transaminase;
AltName:		Full=Putrescine--2-oxoglutaric acid transaminase;

Gene name

patA

Comments [?]

Function	Catalyzes the aminotransferase reaction from putrescine to 2-oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4-aminobutanoate (gamma-aminobutyrate or GABA) via 4-aminobutanal. Also functions as a cadaverine transaminase in a a L-lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate.
Catalytic activity	RHEA:18217: 2-oxoglutarate + an alkane-alpha,omega-diamine = an omega-aminoaldehyde + L-glutamate EC 2.6.1.29 PhysiologicalDirection=left-to-right (RHEA:18218)
	RHEA:12268: 2-oxoglutarate + putrescine = 1-pyrroline + H2O + L-glutamate EC 2.6.1.82 PhysiologicalDirection=left-to-right (RHEA:12269)
	RHEA:61624: 2-oxoglutarate + cadaverine = 5-aminopentanal + L-glutamate PhysiologicalDirection=left-to-right (RHEA:61625)
Cofactor	pyridoxal 5'-phosphate
Pathway	Amine and polyamine degradation; putrescine degradation; 4-aminobutanal from putrescine (transaminase route): step 1/1.
	Amino-acid degradation.
Subunit	Homodimer.
Similarity	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily.

Keywords [?]

Aminotransferase
Pyridoxal phosphate
Transferase

Gene Ontology [?]

GO:0033094; Molecular function: butane-1,4-diamine:2-oxoglutarate aminotransferase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0009447; Biological process: putrescine catabolic process.
GO:0019477; Biological process: L-lysine catabolic process.

Cross-references [?]

PROSITE	PS00600; AA_TRANSFER_CLASS_3; 1;
Pfam	PF00202; Aminotran_3; 1;
NCBIfam	TIGR03372; putres_am_tran; 1;
PIRSF	PIRSF000521; Transaminase_4ab_Lys_Orn; 1;

Features [?]

From: PAT_ECOLI (P42588)

Key From To Description Tag Condition FTGroup

BINDING 150 151 /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain G-T

BINDING 274 274 /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners" /note="in other chain Q

BINDING 332 332 /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326" /ligand_note="ligand shared between dimeric partners T

MOD_RES 300 300 N6-(pyridoxal phosphate)lysine K

Additional information [?]

Size range	459-468 amino acids
Related rules	None
Fusion	None

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