HAMAP rule MF_01289
General rule information
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| Accession | MF_01289 |
| Dates | 19-SEP-2008 (Created)
26-JAN-2023 (Last updated, Version 17) |
| Name | Galacton_dehydrat |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | Q6BF17 (DGOD_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01289 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DGOD |
| Protein name | RecName: Full=D-galactonate dehydratase; Short=GalD; EC=4.2.1.6; |
| Gene name | Name=dgoD; |
Comments
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| FUNCTION | Catalyzes the dehydration of D-galactonate to 2-keto-3-deoxy- D-galactonate. |
| CATALYTIC ACTIVITY | Reaction=D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O; Xref=Rhea:RHEA:18649, ChEBI:CHEBI:12931, ChEBI:CHEBI:15377, ChEBI:CHEBI:57989; EC=4.2.1.6; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| PATHWAY | Carbohydrate acid metabolism; D-galactonate degradation; D- glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 1/3. |
| MISCELLANEOUS | Reaction proceeds via an anti dehydration. |
| SIMILARITY | Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily. |
Keywords
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| Lyase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0008869; Molecular function:galactonate dehydratase activity | |
| GO:0034194; Biological process:D-galactonate catabolic process | |
Cross-references
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| PROSITE | PS00908; MR_MLE_1; 1; |
| PROSITE | PS00909; MR_MLE_2; 1; |
| Pfam | PF01188; MR_MLE; 1; |
| Pfam | PF02746; MR_MLE_N; 1; |
Features
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| From: DGOD_ECOLI (Q6BF17) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 185 | 185 | /note="Proton donor/acceptor" | H | ||||||||
| ACT_SITE | 285 | 285 | /note="Proton donor/acceptor" | H | ||||||||
| BINDING | 183 | 183 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 209 | 209 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 235 | 235 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| SITE | 258 | 258 | /note="Increases basicity of active site His" | D | ||||||||
| SITE | 310 | 310 | /note="Transition state stabilizer" | E | ||||||||
Additional information
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| Size range | 365-385 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |