HAMAP rule MF_01289

General rule information [?]

Accession	MF_01289
Dates	19-SEP-2008 (Created) 26-JAN-2023 (Last updated, Version 17)

Name	Galacton_dehydrat

Scope

Bacteria; Pseudomonadota

Template

Q6BF17 (DGOD_ECOLI)

Triggered by

HAMAP; MF_01289 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DGOD

Protein name

RecName:		Full=D-galactonate dehydratase;
		Short=GalD;
		EC 4.2.1.6;

Gene name

dgoD

Comments [?]

Function	Catalyzes the dehydration of D-galactonate to 2-keto-3-deoxy-D-galactonate.
Catalytic activity	RHEA:18649: D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O EC 4.2.1.6

case <FTGroup:1>

Cofactor

Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.

end case

Pathway	Carbohydrate acid metabolism; D-galactonate degradation; D-glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 1/3.
Miscellaneous	Reaction proceeds via an anti dehydration.
Similarity	Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily.

Keywords [?]

Lyase

case <FTGroup:1>

Magnesium
Metal-binding

end case

Gene Ontology [?]

case <FTGroup:1>

GO:0000287; Molecular function: magnesium ion binding.

end case

GO:0008869; Molecular function: galactonate dehydratase activity.
GO:0034194; Biological process: D-galactonate catabolic process.

Cross-references [?]

PROSITE	PS00908; MR_MLE_1; 1;
	PS00909; MR_MLE_2; 1;
Pfam	PF01188; MR_MLE; 1;
	PF02746; MR_MLE_N; 1;

Features [?]

From: DGOD_ECOLI (Q6BF17)

Key From To Description Tag Condition FTGroup

ACT_SITE 185 185 Proton donor/acceptor H

ACT_SITE 285 285 Proton donor/acceptor H

BINDING 183 183 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D 1

BINDING 209 209 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E 1

BINDING 235 235 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E 1

SITE 258 258 Increases basicity of active site His D

SITE 310 310 Transition state stabilizer E

Additional information [?]

Size range	365-385 amino acids
Related rules	None
Fusion	None

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