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HAMAP rule MF_01297

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General rule information [?]

Accession MF_01297
Dates 25-NOV-2008 (Created)
13-JAN-2023 (Last updated, Version 15)
Name nitrobindin
Scope(s) Bacteria
Template(s) P9WFG9 (NB2_MYCTU); P9WFG7 (NB1_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_01297 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTTag:heme_iron_binding>
Identifier NB
Protein name RecName: Full=Peroxynitrite isomerase;
AltName: Full=Ferric nitrobindin;
Protein name RecName: Full=Ferric nitrobindin-like protein;
end case

Comments [?]

case <FTTag:heme_iron_binding>
FUNCTIONHeme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.
CATALYTIC ACTIVITY Reaction=peroxynitrite = nitrate; Xref=Rhea:RHEA:63116, ChEBI:CHEBI:17632, ChEBI:CHEBI:25941; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63117;
COFACTOR Name=heme b; Xref=ChEBI:CHEBI:60344; Note=Binds 1 heme b group per subunit, that coordinates a highly solvent-exposed Fe(III) atom.
PATHWAYNitrogen metabolism.
DOMAINForms a 10-stranded antiparallel beta-barrel structure able to accommodate a hydrophobic ligand in its interior. In fact, this fold hosts the heme group, which is located in a wide surface cleft.
CAUTIONLacks the conserved His residue that binds heme iron in the nitrobindin family.
end case
SIMILARITYBelongs to the nitrobindin family.

Keywords [?]

case <FTTag:heme_iron_binding>
end case

Gene Ontology [?]

case <FTTag:heme_iron_binding>
GO:0020037; Molecular function:heme binding
GO:0062213; Molecular function:peroxynitrite isomerase activity
end case

Cross-references [?]

Pfam PF08768; DUF1794; 1;

Features [?]

From: NB1_MYCTU (P9WFG7)
Key From To Description Tag Condition FTGroup
MOTIF 17 23 /note="GXWXGXG" G-x-W-x-G-x-G
BINDING 155 155 /ligand="heme b"
/note="axial binding residue"
heme_iron_binding H
BINDING 29 29 /ligand="heme b"
BINDING 123 123 /ligand="heme b"

Additional information [?]

Size range 147-248 amino acids
Related rules None
Fusion Nter: None Cter: None

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