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HAMAP rule MF_01297

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General rule information [?]

Accession MF_01297
Dates 25-NOV-2008 (Created)
13-JAN-2023 (Last updated, Version 15)
Name nitrobindin
Scope
Bacteria; Actinomycetota
Templates P9WFG9 (NB2_MYCTU); P9WFG7 (NB1_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <FTTag:heme_iron_binding>
Identifier
NB
Protein name
RecName: Full=Peroxynitrite isomerase;
EC 5.99.-.-;
AltName: Full=Ferric nitrobindin;
Short=Nb(III);
else
Protein name
RecName: Full=Ferric nitrobindin-like protein;
end case

Comments [?]

case <FTTag:heme_iron_binding>
Function Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.
Catalytic activity RHEA:63116: peroxynitrite = nitrate
PhysiologicalDirection=left-to-right (RHEA:63117)
Cofactor heme b
Note: Binds 1 heme b group per subunit, that coordinates a highly solvent-exposed Fe(III) atom
Pathway Nitrogen metabolism.
Domain Forms a 10-stranded antiparallel beta-barrel structure able to accommodate a hydrophobic ligand in its interior. In fact, this fold hosts the heme group, which is located in a wide surface cleft.
else
Caution Lacks the conserved His residue that binds heme iron in the nitrobindin family.
end case
Similarity Belongs to the nitrobindin family.

Keywords [?]

case <FTTag:heme_iron_binding>
Heme
Iron
Metal-binding
Isomerase
end case

Gene Ontology [?]

case <FTTag:heme_iron_binding>
GO:0020037; Molecular function: heme binding.
GO:0062213; Molecular function: peroxynitrite isomerase activity.
end case

Cross-references [?]

Pfam PF08768; DUF1794; 1;

Features [?]

From: NB1_MYCTU (P9WFG7)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     17     23       GXWXGXG     G-x-W-x-G-x-G  
BINDING     155     155       /ligand="heme b" /ligand_id="ChEBI:CHEBI:60344" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue   heme_iron_binding   H  
BINDING (Optional)     29     29       /ligand="heme b" /ligand_id="ChEBI:CHEBI:60344     T  
BINDING (Optional)     123     123       /ligand="heme b" /ligand_id="ChEBI:CHEBI:60344     K  

Additional information [?]

Size range 147-248 amino acids
Related rules None
Fusion None


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