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Annotation rule MF_01324
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General rule information [?]

Accession MF_01324
Dates 6-JAN-2004 (Created)
19-NOV-2019 (Last updated, Version 32)
Name RNApol_bact_RpoC2
Scope
Bacteria; Cyanobacteria
Plastid
Template P22705 (RPOC2_NOSS1)
case <OC:Cyanobacteria> or <OG:Chloroplast>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RPOC2
case <OC:Cyanobacteria>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit beta';
Short=RNAP subunit beta';
EC 2.7.7.6;
AltName: Full=RNA polymerase subunit beta';
AltName: Full=Transcriptase subunit beta';
else case <OG:Chloroplast>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit beta'';
EC 2.7.7.6;
AltName: Full=PEP;
AltName: Full=Plastid-encoded RNA polymerase subunit beta'';
Short=RNA polymerase subunit beta'';
end case
Gene name
rpoC2

Comments [?]

Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 Zn(2+) ion per subunit.
end case
case <OC:Cyanobacteria>
Subunit In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
else case <OG:Chloroplast>
Subunit In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.

Keywords [?]

case <FTGroup:1>
Metal-binding
Zinc
end case
Transcription
Transferase
Nucleotidyltransferase
DNA-directed RNA polymerase

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-templated.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF04983; RNA_pol_Rpb1_3; 0-1;
PF05000; RNA_pol_Rpb1_4; 1;
PF04998; RNA_pol_Rpb1_5; 1-2;
TIGRFAMs TIGR02388; rpoC2_cyan; 0-2;

Features [?]

From: RPOC2_NOSS1 (P22705)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     219     219       Zinc     C   1
METAL     293     293       Zinc     C   1
METAL     300     300       Zinc     C   1
METAL     303     303       Zinc     C   1

Additional information [?]

Size range 1011-1595 amino acids
Related rules MF_01322 (RPOC supersedes the current rule)
Fusion Nter: MF_01323 (rpoC1); Cter: None
Comments In cyanobacteria and plastids, there are two ORFs (rpoC1, MF_01323 and this one, rpoC2, MF_01324) that together are equivalent to the beta' subunit found in all other bacteria (rpoC, MF_01322). However,in the red algae CYAM1 the rpoC1 and rpoC2 genes are fused into one ORF. This seems to be the result of evolution acting to reduce protein size rather than horizontal transfer from a bacteria. Some algal proteins with inserts are not included in the alignment. ASTLO and EUGGR have large deletions and are not included in the alignments.


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