HAMAP logo

HAMAP rule MF_01324

Send feedback

General rule information [?]

Accession MF_01324
Dates 6-JAN-2004 (Created)
1-JUN-2023 (Last updated, Version 38)
Name RNApol_bact_RpoC2
Scope(s) Bacteria
Template(s) P22705 (RPOC2_NOSS1); [ Recover all ]
Triggered by
case c? <OC:Cyanobacteriota> or <OG:Chloroplast>
HAMAP; MF_01324 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RPOC2
case <OC:Cyanobacteriota>
Protein name RecName: Full=DNA-directed RNA polymerase subunit beta';
                 Short=RNAP subunit beta';
AltName: Full=RNA polymerase subunit beta';
AltName: Full=Transcriptase subunit beta';
else case <OG:Chloroplast>
Protein name RecName: Full=DNA-directed RNA polymerase subunit beta'';
AltName: Full=PEP;
AltName: Full=Plastid-encoded RNA polymerase subunit beta'';
                 Short=RNA polymerase subunit beta'';
end case
Gene name Name=rpoC2;

Comments [?]

FUNCTIONDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
CATALYTIC ACTIVITY Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 Zn(2+) ion per subunit.;
end case
case <OC:Cyanobacteriota>
SUBUNITIn cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
else case <OG:Chloroplast>
SUBUNITIn plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
SIMILARITYBelongs to the RNA polymerase beta' chain family. RpoC2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
GO:0003899; Molecular function:DNA-directed 5'-3' RNA polymerase activity
GO:0006351; Biological process:DNA-templated transcription
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam PF04983; RNA_pol_Rpb1_3; 0-1;
Pfam PF05000; RNA_pol_Rpb1_4; 1;
Pfam PF04998; RNA_pol_Rpb1_5; 1-2;
NCBIfam TIGR02388; rpoC2_cyan; 0-2;

Features [?]

From: RPOC2_NOSS1 (P22705)
Key From To Description Tag Condition FTGroup
BINDING 219 219 /ligand="Zn(2+)"
C 1
BINDING 293 293 /ligand="Zn(2+)"
C 1
BINDING 300 300 /ligand="Zn(2+)"
C 1
BINDING 303 303 /ligand="Zn(2+)"
C 1

Additional information [?]

Size range 1011-1595 amino acids
Related rules MF_01322
Fusion Nter: MF_01323 (rpoC1) Cter: None
Comments In cyanobacteria and plastids, there are two ORFs (rpoC1, MF_01323 and this one, rpoC2, MF_01324) that together are equivalent to the beta' subunit found in all other bacteria (rpoC, MF_01322). However,in the red algae CYAM1 the rpoC1 and rpoC2 genes are fused into one ORF. This seems to be the result of evolution acting to reduce protein size rather than horizontal transfer from a bacteria. Some algal proteins with inserts are not included in the alignment. ASTLO and EUGGR have large deletions and are not included in the alignments.

View rule in raw text format (no links)