 |
|
Annotation rule MF_01324 |
General rule information
[?]
Accession |
MF_01324 |
Dates |
6-JAN-2004 (Created) 19-NOV-2019 (Last updated, Version 32) |
Scope |
Bacteria; Cyanobacteria
Plastid |
case <OC:Cyanobacteria> or <OG:Chloroplast>
end case
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Cyanobacteria>
Protein name |
RecName:
|
Full=DNA-directed RNA polymerase subunit beta';
|
|
Short=RNAP subunit beta';
|
|
EC 2.7.7.6;
|
AltName:
|
Full=RNA polymerase subunit beta';
|
AltName:
|
Full=Transcriptase subunit beta';
|
|
else case <OG:Chloroplast>
Protein name |
RecName:
|
Full=DNA-directed RNA polymerase subunit beta'';
|
|
EC 2.7.7.6;
|
AltName:
|
Full=PEP;
|
AltName:
|
Full=Plastid-encoded RNA polymerase subunit beta'';
|
|
Short=RNA polymerase subunit beta'';
|
|
end case
Function |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
Catalytic activity |
RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6
|
case <FTGroup:1>
Cofactor |
Zn(2+) Note: Binds 1 Zn(2+) ion per subunit. |
end case
case <OC:Cyanobacteria>
Subunit |
In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
else case <OG:Chloroplast>
Subunit |
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
Subcellular location |
Plastid, chloroplast. |
end case
Similarity |
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily. |
case <FTGroup:1>
end case
GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-templated.
case <FTGroup:1>
end case
case <OG:Chloroplast>
end case
From: RPOC2_NOSS1 (P22705) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
METAL
|
|
219
|
|
219
|
|
Zinc
|
|
|
|
C
|
|
1
|
METAL
|
|
293
|
|
293
|
|
Zinc
|
|
|
|
C
|
|
1
|
METAL
|
|
300
|
|
300
|
|
Zinc
|
|
|
|
C
|
|
1
|
METAL
|
|
303
|
|
303
|
|
Zinc
|
|
|
|
C
|
|
1
|
Additional information
[?]
Size range |
1011-1595 amino acids |
Related rules |
MF_01322 (RPOC supersedes the current rule) |
Fusion |
Nter: MF_01323 (rpoC1); Cter: None |
Comments |
In cyanobacteria and plastids, there are two ORFs (rpoC1, MF_01323 and this one, rpoC2, MF_01324) that together are equivalent to the beta' subunit found in all other bacteria (rpoC, MF_01322). However,in the red algae CYAM1 the rpoC1 and rpoC2 genes are fused into one ORF. This seems to be the result of evolution acting to reduce protein size rather than horizontal transfer from a bacteria. Some algal proteins with inserts are not included in the alignment. ASTLO and EUGGR have large deletions and are not included in the alignments. |