HAMAP rule MF_01327

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01327
Accession	MF_01327
Dates	28-FEB-2005 (Created) 02-SEP-2024 (Last updated, Version 35)
Name	TMP_synthase_cyanobact
Scope(s)	Bacteria Cyanobacteriota
Template(s)	Q8YX72; [ Recover all ]
Triggered by	HAMAP; MF_01327 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	THIE
Protein name	RecName: Full=Thiamine-phosphate synthase; Short=TP synthase; Short=TPS; EC=2.5.1.3; AltName: Full=Thiamine-phosphate pyrophosphorylase; Short=TMP pyrophosphorylase; Short=TMP-PPase;
Gene name	Name=thiE;

Comments [?]

FUNCTION	Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
CATALYTIC ACTIVITY	Reaction=2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate; Xref=Rhea:RHEA:47844, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62899; EC=2.5.1.3;
CATALYTIC ACTIVITY	Reaction=2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2- methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate; Xref=Rhea:RHEA:47848, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62890; EC=2.5.1.3;
CATALYTIC ACTIVITY	Reaction=4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate; Xref=Rhea:RHEA:22328, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:58296; EC=2.5.1.3;
case <FTGroup:1>
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAY	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.
SIMILARITY	Belongs to the thiamine-phosphate synthase family.

Keywords [?]

Thiamine biosynthesis
Transferase
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding

GO:0004789; Molecular function:thiamine-phosphate diphosphorylase activity

GO:0009229; Biological process:thiamine diphosphate biosynthetic process

Cross-references [?]

Pfam	PF02581; TMP-TENI; 1;
NCBIfam	TIGR00693; ThiE; 1;
PIRSF	PIRSF000512; TMP_PPase_Cyanobac_prd; 1;

Features [?]

From: THIE_NOSS1 (Q8YX72)

Key

From

Description

Tag

Condition

FTGroup

REGION

Nter

129

/note="Unknown"

REGION

130

Cter

/note="Thiamine-phosphate synthase"

BINDING

177

181

/ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"

Q-x-R-x-K

BINDING

274

276

/ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)- ylidene]ethyl phosphate"
/ligand_id="ChEBI:CHEBI:62899"

T-x-T

BINDING

210

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

[DN]

BINDING

229

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

[DE]

BINDING

209

/ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"

BINDING

248

/ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"

BINDING

277

/ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"

BINDING

304

/ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)- ylidene]ethyl phosphate"
/ligand_id="ChEBI:CHEBI:62899"

Additional information [?]

Size range	341-353 amino acids
Related rules	MF_00097
Fusion	Nter: None Cter: None
Comments	Unknown N-terminal domain in Cyanobacteriota. All other bacteria without this domain belong to family MF_00097.

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