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HAMAP rule MF_01327

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General rule information [?]

Accession MF_01327
Dates 19-FEB-2001 (Created)
2-SEP-2024 (Last updated, Version 35)
Name TMP_synthase_cyanobact
Scope(s) Bacteria
Cyanobacteriota
Template(s) Q8YX72 (THIE_NOSS1); [ Recover all ]
Triggered by HAMAP; MF_01327 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier THIE
Protein name RecName: Full=Thiamine-phosphate synthase;
                 Short=TP synthase;
                 Short=TPS;
                 EC=2.5.1.3;
AltName: Full=Thiamine-phosphate pyrophosphorylase;
                 Short=TMP pyrophosphorylase;
                 Short=TMP-PPase;
Gene name Name=thiE;

Comments [?]

FUNCTIONCondenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
CATALYTIC ACTIVITY Reaction=2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate; Xref=Rhea:RHEA:47844, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62899; EC=2.5.1.3;
CATALYTIC ACTIVITY Reaction=2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2- methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate; Xref=Rhea:RHEA:47848, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:62890; EC=2.5.1.3;
CATALYTIC ACTIVITY Reaction=4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate; Xref=Rhea:RHEA:22328, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37575, ChEBI:CHEBI:57841, ChEBI:CHEBI:58296; EC=2.5.1.3;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYCofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.
SIMILARITYBelongs to the thiamine-phosphate synthase family.

Keywords [?]

Thiamine biosynthesis
Transferase
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0004789; Molecular function:thiamine-phosphate diphosphorylase activity
GO:0009229; Biological process:thiamine diphosphate biosynthetic process

Cross-references [?]

Pfam PF02581; TMP-TENI; 1;
NCBIfam TIGR00693; ThiE; 1;
PIRSF PIRSF000512; TMP_PPase_Cyanobac_prd; 1;

Features [?]

From: THIE_NOSS1 (Q8YX72)
Key From To Description Tag Condition FTGroup
REGION Nter 129 /note="Unknown"
REGION 130 Cter /note="Thiamine-phosphate synthase"
BINDING 177 181 /ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"		 Q-x-R-x-K
BINDING 274 276 /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)- ylidene]ethyl phosphate"
/ligand_id="ChEBI:CHEBI:62899"		 T-x-T
BINDING 210 210 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [DN] 1
BINDING 229 229 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [DE] 1
BINDING 209 209 /ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"		 N
BINDING 248 248 /ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"		 S
BINDING 277 277 /ligand="4-amino-2-methyl-5- (diphosphooxymethyl)pyrimidine"
/ligand_id="ChEBI:CHEBI:57841"		 K
BINDING 304 304 /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)- ylidene]ethyl phosphate"
/ligand_id="ChEBI:CHEBI:62899"		 G

Additional information [?]

Size range 341-353 amino acids
Related rules MF_00097
Fusion Nter: None Cter: None
Comments Unknown N-terminal domain in Cyanobacteriota. All other bacteria without this domain belong to family MF_00097.



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