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HAMAP rule MF_01327

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General rule information [?]

Accession MF_01327
Dates 19-FEB-2001 (Created)
1-JUN-2023 (Last updated, Version 33)
Name TMP_synthase_cyanobact
Scope
Bacteria; Cyanobacteriota
Template Q8YX72 (THIE_NOSS1)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
THIE
Protein name
RecName: Full=Thiamine-phosphate synthase;
Short=TP synthase;
Short=TPS;
EC 2.5.1.3;
AltName: Full=Thiamine-phosphate pyrophosphorylase;
Short=TMP pyrophosphorylase;
Short=TMP-PPase;
Gene name
thiE

Comments [?]

Function Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
Catalytic activity RHEA:47844: 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate
EC 2.5.1.3
RHEA:47848: 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate
EC 2.5.1.3
RHEA:22328: 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate
EC 2.5.1.3
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Pathway Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.
Similarity Belongs to the thiamine-phosphate synthase family.

Keywords [?]

Thiamine biosynthesis
Transferase
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004789; Molecular function: thiamine-phosphate diphosphorylase activity.
GO:0009228; Biological process: thiamine biosynthetic process.

Cross-references [?]

Pfam PF02581; TMP-TENI; 1;
NCBIfam TIGR00693; ThiE; 1;
PIRSF PIRSF000512; TMP_PPase_Cyanobac_prd; 1;

Features [?]

From: THIE_NOSS1 (Q8YX72)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     129       Unknown        
REGION     130     Cter       Thiamine-phosphate synthase        
BINDING     177     181       /ligand="4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine" /ligand_id="ChEBI:CHEBI:57841     Q-x-R-x-K  
BINDING (Optional)     274     276       /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate" /ligand_id="ChEBI:CHEBI:62899     T-x-T  
BINDING     210     210       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     [DN]   1
BINDING     229     229       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     [DE]   1
BINDING     209     209       /ligand="4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine" /ligand_id="ChEBI:CHEBI:57841     N  
BINDING     248     248       /ligand="4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine" /ligand_id="ChEBI:CHEBI:57841     S  
BINDING     277     277       /ligand="4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine" /ligand_id="ChEBI:CHEBI:57841     K  
BINDING     304     304       /ligand="2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate" /ligand_id="ChEBI:CHEBI:62899     G  

Additional information [?]

Size range 341-353 amino acids
Related rules MF_00097 (THIE)
Fusion None
Comments Unknown N-terminal domain in Cyanobacteriota. All other bacteria without this domain belong to family MF_00097.


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