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HAMAP rule MF_01349

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01349
Accession MF_01349
Dates 29-MAY-2006 (Created)
12-JUN-2025 (Last updated, Version 26)
Name PanCY
Scope(s) Bacteria
Cyanobacteriota
Template(s) P9WIL5; P0A6I0; [ Recover all ]
Triggered by HAMAP; MF_01349 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PANCY
Protein name RecName: Full=Bifunctional pantoate ligase/cytidylate kinase;
                 Includes:
RecName: Full=Pantothenate synthetase;
                 Short=PS;
                 EC=6.3.2.1;
AltName: Full=Pantoate--beta-alanine ligase;
AltName: Full=Pantoate-activating enzyme;
                 Includes:
RecName: Full=Cytidylate kinase;
                 Short=CK;
                 EC=2.7.4.25;
AltName: Full=Cytidine monophosphate kinase;
                 Short=CMP kinase;
Gene name Name=panC/cmk;

Comments [?]

FUNCTIONCatalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.
FUNCTIONCatalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively.
CATALYTIC ACTIVITY Reaction=(R)-pantoate + beta-alanine + ATP = (R)-pantothenate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10912, ChEBI:CHEBI:15378, ChEBI:CHEBI:15980, ChEBI:CHEBI:29032, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57966, ChEBI:CHEBI:456215; EC=6.3.2.1;
CATALYTIC ACTIVITY Reaction=CMP + ATP = CDP + ADP; Xref=Rhea:RHEA:11600, ChEBI:CHEBI:30616, ChEBI:CHEBI:58069, ChEBI:CHEBI:60377, ChEBI:CHEBI:456216; EC=2.7.4.25;
CATALYTIC ACTIVITY Reaction=dCMP + ATP = dCDP + ADP; Xref=Rhea:RHEA:25094, ChEBI:CHEBI:30616, ChEBI:CHEBI:57566, ChEBI:CHEBI:58593, ChEBI:CHEBI:456216; EC=2.7.4.25;
PATHWAYCofactor biosynthesis; (R)-pantothenate biosynthesis; (R)- pantothenate from (R)-pantoate and beta-alanine: step 1/1.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYIn the N-terminal section; belongs to the pantothenate synthetase family.
SIMILARITYIn the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Keywords [?]

Cytoplasm
ATP-binding
Kinase
Ligase
Multifunctional enzyme
Nucleotide-binding
Pantothenate biosynthesis
Transferase

Gene Ontology [?]

GO:0004592; Molecular function:pantoate-beta-alanine ligase activity
GO:0005524; Molecular function:ATP binding
GO:0036430; Molecular function:CMP kinase activity
GO:0036431; Molecular function:dCMP kinase activity
GO:0006220; Biological process:pyrimidine nucleotide metabolic process
GO:0015940; Biological process:pantothenate biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF02224; Cytidylate_kin; 1;
Pfam PF02569; Pantoate_ligase; 1;
NCBIfam TIGR00017; Cmk; 1;
NCBIfam TIGR00018; PanC; 1;
NCBIfam TIGR00125; Cyt_tran_rel; 1;

Features [?]

From: PANCY_SYNY3 (Q55074)
Key From To Description Tag Condition FTGroup
BINDING 30 37 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 M-x(3)-H-x(2)-H
BINDING 150 153 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 G-x(2)-D
BINDING 187 190 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 x(3)-R
REGION Nter 283 /note="Pantoate--beta-alanine ligase"
REGION 284 Cter /note="Cytidylate kinase"
ACT_SITE 37 37 /note="Proton donor" H
BINDING 61 61 /ligand="beta-alanine"
/ligand_id="ChEBI:CHEBI:57966"		 Q
BINDING 61 61 /ligand="(R)-pantoate"
/ligand_id="ChEBI:CHEBI:15980"		 Q
BINDING 156 156 /ligand="(R)-pantoate"
/ligand_id="ChEBI:CHEBI:15980"		 Q
BINDING 179 179 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [VLIGA]

Additional information [?]

Size range 480-542 amino acids
Related rules MF_00158
MF_00238
MF_00239
Fusion Nter: None Cter: None
Comments This fusion protein seems only to exist in Cyanobacteriota; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity.



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