HAMAP rule MF_01349

General rule information [?]

Accession	MF_01349
Dates	29-MAY-2006 (Created) 1-JUN-2023 (Last updated, Version 22)

Name

PanCY

Scope

Bacteria; Cyanobacteriota

Templates

P9WIL5 (PANC_MYCTU); P0A6I0 (KCY_ECOLI): [Recover all]

Triggered by

HAMAP; MF_01349 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PANCY

Protein name

RecName:		Full=Bifunctional pantoate ligase/cytidylate kinase;
Includes:
	RecName:		Full=Pantothenate synthetase;
			Short=PS;
			EC 6.3.2.1;
	AltName:		Full=Pantoate--beta-alanine ligase;
	AltName:		Full=Pantoate-activating enzyme;
Includes:
	RecName:		Full=Cytidylate kinase;
			Short=CK;
			EC 2.7.4.25;
	AltName:		Full=Cytidine monophosphate kinase;
			Short=CMP kinase;

Gene name

panC/cmk

Comments [?]

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.
	Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively.
Catalytic activity	RHEA:10912: (R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+) EC 6.3.2.1
	RHEA:11600: ATP + CMP = ADP + CDP EC 2.7.4.25
	RHEA:25094: ATP + dCMP = ADP + dCDP EC 2.7.4.25
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.
Subcellular location	Cytoplasm.
Similarity	In the N-terminal section; belongs to the pantothenate synthetase family.
	In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Keywords [?]

Cytoplasm
ATP-binding
Kinase
Ligase
Multifunctional enzyme
Nucleotide-binding
Pantothenate biosynthesis
Transferase

Gene Ontology [?]

GO:0004592; Molecular function: pantoate-beta-alanine ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0004127; Molecular function: cytidylate kinase activity.
GO:0006220; Biological process: pyrimidine nucleotide metabolic process.
GO:0015940; Biological process: pantothenate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF02224; Cytidylate_kin; 1;
	PF02569; Pantoate_ligase; 1;
NCBIfam	TIGR00017; Cmk; 1;
	TIGR00018; PanC; 1;
	TIGR00125; Cyt_tran_rel; 1;

Features [?]

From: PANCY_SYNY3 (Q55074)

Key From To Description Tag Condition FTGroup

BINDING 30 37 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 M-x(3)-H-x(2)-H

BINDING 150 153 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-x(2)-D

BINDING 187 190 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 x(3)-R

REGION Nter 283 Pantoate--beta-alanine ligase

REGION 284 Cter Cytidylate kinase

ACT_SITE 37 37 Proton donor H

BINDING 61 61 /ligand="beta-alanine" /ligand_id="ChEBI:CHEBI:57966 Q

BINDING 61 61 /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980 Q

BINDING 156 156 /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980 Q

BINDING (Optional) 179 179 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [VLIGA]

Additional information [?]

Size range	480-542 amino acids
Related rules	MF_00158 (PANC); MF_00238 (KCY); MF_00239 (KCY)
Fusion	None
Comments	This fusion protein seems only to exist in Cyanobacteriota; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity.

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