HAMAP logo

HAMAP rule MF_01349

Send feedback

General rule information [?]

Accession MF_01349
Dates 29-MAY-2006 (Created)
1-JUN-2023 (Last updated, Version 22)
Name PanCY
Bacteria; Cyanobacteriota
Templates P9WIL5 (PANC_MYCTU); P0A6I0 (KCY_ECOLI): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Bifunctional pantoate ligase/cytidylate kinase;
RecName: Full=Pantothenate synthetase;
AltName: Full=Pantoate--beta-alanine ligase;
AltName: Full=Pantoate-activating enzyme;
RecName: Full=Cytidylate kinase;
AltName: Full=Cytidine monophosphate kinase;
Short=CMP kinase;
Gene name

Comments [?]

Function Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.
Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively.
Catalytic activity RHEA:10912: (R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+)
RHEA:11600: ATP + CMP = ADP + CDP
RHEA:25094: ATP + dCMP = ADP + dCDP
Pathway Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the pantothenate synthetase family.
In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004592; Molecular function: pantoate-beta-alanine ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0004127; Molecular function: cytidylate kinase activity.
GO:0006220; Biological process: pyrimidine nucleotide metabolic process.
GO:0015940; Biological process: pantothenate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02224; Cytidylate_kin; 1;
PF02569; Pantoate_ligase; 1;
NCBIfam TIGR00017; Cmk; 1;
TIGR00018; PanC; 1;
TIGR00125; Cyt_tran_rel; 1;

Features [?]

From: PANCY_SYNY3 (Q55074)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     30     37       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     M-x(3)-H-x(2)-H  
BINDING     150     153       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-x(2)-D  
BINDING     187     190       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     x(3)-R  
REGION     Nter     283       Pantoate--beta-alanine ligase        
REGION     284     Cter       Cytidylate kinase        
ACT_SITE     37     37       Proton donor     H  
BINDING     61     61       /ligand="beta-alanine" /ligand_id="ChEBI:CHEBI:57966     Q  
BINDING     61     61       /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980     Q  
BINDING     156     156       /ligand="(R)-pantoate" /ligand_id="ChEBI:CHEBI:15980     Q  
BINDING (Optional)     179     179       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [VLIGA]  

Additional information [?]

Size range 480-542 amino acids
Related rules MF_00158 (PANC); MF_00238 (KCY); MF_00239 (KCY)
Fusion None
Comments This fusion protein seems only to exist in Cyanobacteriota; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity.