HAMAP rule MF_01364
General rule information
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| Accession | MF_01364 |
| Dates | 24-NOV-2006 (Created)
17-MAR-2023 (Last updated, Version 27) |
| Name | Ribosomal_S14_2 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0DOY6 (RS14Z_THET8); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria> or <OG:Chloroplast>
HAMAP; MF_01364_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_01364_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Bacteria> and not <OC:Cyanobacteriota> | |
| Identifier | RS14Z |
| Protein name | RecName: Full=Small ribosomal subunit protein uS14; |
| Gene name | Name=rpsZ; Synonyms=rpsN; |
| else case <OC:Cyanobacteriota> | |
| Identifier | RS14Z |
| Protein name | RecName: Full=Small ribosomal subunit protein uS14; |
| Gene name | Name=rpsZ; Synonyms=rps14, rpsN; |
| else case <OC:Archaea> | |
| Identifier | RS14Z |
| Protein name | RecName: Full=Small ribosomal subunit protein uS14; |
| Gene name | Name=rps14; |
| end case | |
Comments
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| case <OC:Bacteria> | |
| FUNCTION | Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. |
| SUBUNIT | Part of the 30S ribosomal subunit. Contacts proteins S3 and S10. |
| else | |
| FUNCTION | Binds 16S rRNA, required for the assembly of 30S particles. |
| SUBUNIT | Part of the 30S ribosomal subunit. |
| end case | |
| case <FTGroup:1> or <FTGroup:2> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| SIMILARITY | Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily. |
Keywords
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| Ribonucleoprotein | |
| Ribosomal protein | |
| RNA-binding | |
| rRNA-binding | |
| case <FTGroup:1> or <FTGroup:2> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0006412; Biological process:translation | |
| GO:0019843; Molecular function:rRNA binding | |
| case <FTGroup:1> or <FTGroup:2> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
Cross-references
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Features
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| From: RS14Z_THET8 (P0DOY6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| BINDING | 24 | 24 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 27 | 27 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 40 | 40 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 43 | 43 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| end case | ||||||||||||
| From: RS14Z_HALMA (P26816) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| BINDING | 26 | 26 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 2 | |||||||
| BINDING | 29 | 29 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 2 | |||||||
| BINDING | 44 | 44 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 2 | |||||||
| BINDING | 47 | 47 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 2 | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 47-69 amino acids |
| Related rules |
MF_00537 |
| Fusion | Nter: None Cter: None |
| Comments | In bacteria this gene has been subject to horizontal gene transfer, subsequent differential gene loss and insertions/deletions near the N-terminus. The ancestral version is most likely the shortest one (group I of PubMed 11102698) which has conserved cysteine residues that in the case of Thermus thermophilus have been shown to bind zinc. We have chosen to use the identifier RS14Z in bacteria for the zinc-binding subfamily (this family, MF_01364). The group II proteins have an approximately 23 amino acid insertion and the group III proteins have an approximately 38 residue insertion; we do not distinguish between them however (MF_00537); members of this subfamily do not usually possess the conserved cysteine residues. Many bacteria possess more than one copy of RS14, usually one of which is able to bind zinc. Interestingly, in the proteobacteria (Pseudomonadota, which usually have the group III form) the acquired form is found within the spc operon, signifying gene replacement took place within the operon. See: PubMed=11102698; Brochier C., Philippe H., Moreira D.; "The evolutionary history of ribosomal protein RpS14: horizontal gene transfer at the heart of the ribosome."; Trends Genet. 16:529-533(2000). PubMed=11574053; Makarova K.S., Ponomarev V.A., Koonin E.V.; "Two C or not two C: recurrent disruption of Zn-ribbons, gene duplication, lineage-specific gene loss, and horizontal gene transfer in evolution of bacterial ribosomal proteins."; Genome Biol. 2:RESEARCH 0033-RESEARCH 0033(2001). |