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Annotation rule MF_01364
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General rule information [?]

Accession MF_01364
Dates 24-NOV-2006 (Created)
19-NOV-2019 (Last updated, Version 23)
Name Ribosomal_S14_2
Scope
Bacteria
Archaea
Template P0DOY6 (RS14Z_THET8)
case <OC:Bacteria> or <OG:Chloroplast>
Triggered by
end case

case <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Bacteria> and not <OC:Cyanobacteria>
Identifier
RS14Z
Protein name
RecName: Full=30S ribosomal protein S14 type Z;
Gene name
rpsZ, rpsN
else case <OC:Cyanobacteria>
Identifier
RS14Z
Protein name
RecName: Full=30S ribosomal protein S14 type Z;
Gene name
rpsZ, rps14, rpsN
else case <OC:Archaea>
Identifier
RS14Z
Protein name
RecName: Full=30S ribosomal protein S14 type Z;
Gene name
rps14
end case

Comments [?]

case <OC:Bacteria>
Function Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.
Subunit Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.
else
Function Binds 16S rRNA, required for the assembly of 30S particles.
Subunit Part of the 30S ribosomal subunit.
end case
case <FTGroup:1> or <FTGroup:2>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
Similarity Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.

Keywords [?]

Ribonucleoprotein
Ribosomal protein
RNA-binding
rRNA-binding
case <FTGroup:1> or <FTGroup:2>
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0006412; Biological process: translation.
GO:0019843; Molecular function: rRNA binding.
case <FTGroup:1> or <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

PROSITE PS00527; RIBOSOMAL_S14; 1;
Pfam PF00253; Ribosomal_S14; 1;

Features [?]

case <OC:Bacteria>
From: RS14Z_THET8 (P0DOY6)
Key     From     To       Description   Tag   Condition   FTGroup
METAL (Optional)     24     24       Zinc     C   1
METAL (Optional)     27     27       Zinc     C   1
METAL (Optional)     40     40       Zinc     C   1
METAL (Optional)     43     43       Zinc     C   1
end case
case <OC:Archaea>
From: RS14Z_HALMA (P26816)
METAL (Optional)     26     26       Zinc     C   2
METAL (Optional)     29     29       Zinc     C   2
METAL (Optional)     44     44       Zinc     C   2
METAL (Optional)     47     47       Zinc     C   2
end case

Additional information [?]

case <OC:Bacteria>
Size range 60-62 amino acids
end case
case <OC:Archaea>
Size range 47-69 amino acids
end case
Related rules MF_00537 (RS14 supersedes the current rule)
Fusion None
Comments In bacteria this gene has been subject to horizontal gene transfer, subsequent differential gene loss and insertions/deletions near the N-terminus. The ancestral version is most likely the shortest one (group I of PubMed 11102698) which has conserved cysteine residues that in the case of Thermus thermophilus have been shown to bind zinc. We have chosen to use the identifier RS14Z in bacteria for the zinc-binding subfamily (this family, MF_01364). The group II proteins have an approximately 23 amino acid insertion and the group III proteins have an approximately 38 residue insertion; we do not distinguish between them however (MF_00537); members of this subfamily do not usually possess the conserved cysteine residues. Many bacteria possess more than one copy of RS14, usually one of which is able to bind zinc. Interestingly, in the proteobacteria (which usually have the group III form) the acquired form is found within the spc operon, signifying gene replacement took place within the operon. See: PubMed=11102698; Brochier C., Philippe H., Moreira D.; "The evolutionary history of ribosomal protein RpS14: horizontal gene transfer at the heart of the ribosome."; Trends Genet. 16:529-533(2000). PubMed=11574053; Makarova K.S., Ponomarev V.A., Koonin E.V.; "Two C or not two C: recurrent disruption of Zn-ribbons, gene duplication, lineage-specific gene loss, and horizontal gene transfer in evolution of bacterial ribosomal proteins."; Genome Biol. 2:RESEARCH 0033-RESEARCH 0033(2001).


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