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HAMAP rule MF_01397

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General rule information [?]

Accession MF_01397
Dates 29-JAN-2009 (Created)
28-JUN-2024 (Last updated, Version 14)
Name NDH1_NuoCD_2
Scope(s) Bacteria
Aquificia
Bacteroidia
Deltaproteobacteria
Campylobacteraceae
Template(s) P33599 (NUOCD_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01397 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NUOCD
Protein name RecName: Full=NADH-quinone oxidoreductase subunit C/D;
                 EC=7.1.1.-;
AltName: Full=NADH dehydrogenase I subunit C/D;
AltName: Full=NDH-1 subunit C/D;
AltName: Full=NUO3/NUO4;
Gene name Name=nuoC;

Comments [?]

FUNCTIONNDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
CATALYTIC ACTIVITY Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
SUBUNITNDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
SUBCELLULAR LOCATIONCell inner membrane; Peripheral membrane protein.
SIMILARITYIn the N-terminal section; belongs to the complex I 30 kDa subunit family.
SIMILARITYIn the C-terminal section; belongs to the complex I 49 kDa subunit family.

Keywords [?]

Cell inner membrane
Cell membrane
Cytoplasm
Membrane
Multifunctional enzyme
NAD
Quinone
<a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a>
Transport
Ubiquinone

Gene Ontology [?]

GO:0016655; Molecular function:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
GO:0050136; Molecular function:NADH:ubiquinone reductase (non-electrogenic) activity
GO:0005886; Cellular component:plasma membrane

Cross-references [?]

PROSITE PS00542; COMPLEX1_30K; 1;
PROSITE PS00535; COMPLEX1_49K; 1;
Pfam PF00329; Complex1_30kDa; 1;
Pfam PF00346; Complex1_49kDa; 1;
NCBIfam TIGR01961; NuoC_fam; 1;
NCBIfam TIGR01962; NuoD; 1;

Features [?]

From: NUOCD_BDEBA (Q6MIR5)
Key From To Description Tag Condition FTGroup
REGION Nter 157 /note="NADH dehydrogenase I subunit C"
REGION 175 Cter /note="NADH dehydrogenase I subunit D"

Additional information [?]

Size range 519-593 amino acids
Related rules MF_01357
MF_01358
MF_01359
Fusion Nter: None Cter: None
Comments This family is specific for the nuoC-nuoD fusion found in Aquificia, Bacteroidetes, Delta-and Epsilonproteobacteria. The nuoCD fusion protein found in the Alpha-, Beta- and Gammaproteobacteria is different enough that it seems to be another subfamily (MF_01359). The individual families are MF_01357 (nuoC) and MF_01358 (nuoD). In many bacteria there are both the individual and fused versions. 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature.



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