HAMAP rule MF_01405
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01405 |
| Accession | MF_01405 |
| Dates | 8-AUG-2002 (Created)
21-FEB-2025 (Last updated, Version 33) |
| Name | Non_canon_purine_NTPase |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P52061 (IXTPA_ECOLI); Q57679 (IXTPA_METJA); O59580 (IXTPA_PYRHO); O28046 (IXTPA_ARCFU); [ Recover all ] |
| Triggered by |
HAMAP; MF_01405 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | IXTPA |
| Protein name | RecName: Full=dITP/XTP pyrophosphatase; EC=3.6.1.66; AltName: Full=Non-canonical purine NTP pyrophosphatase; AltName: Full=Non-standard purine NTP pyrophosphatase; AltName: Full=Nucleoside-triphosphate diphosphatase; AltName: Full=Nucleoside-triphosphate pyrophosphatase; Short=NTPase; |
Comments
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| FUNCTION | Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. |
| CATALYTIC ACTIVITY | Reaction=XTP + H2O = XMP + diphosphate + H(+); Xref=Rhea:RHEA:28610, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:61314; EC=3.6.1.66; |
| CATALYTIC ACTIVITY | Reaction=dITP + H2O = dIMP + diphosphate + H(+); Xref=Rhea:RHEA:28342, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61194, ChEBI:CHEBI:61382; EC=3.6.1.66; |
| CATALYTIC ACTIVITY | Reaction=ITP + H2O = IMP + diphosphate + H(+); Xref=Rhea:RHEA:29399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58053, ChEBI:CHEBI:61402; EC=3.6.1.66; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the HAM1 NTPase family. |
Keywords
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Gene Ontology
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| GO:0047429; Molecular function:nucleoside triphosphate diphosphatase activity |
| GO:0036220; Molecular function:ITP diphosphatase activity |
| GO:0035870; Molecular function:dITP diphosphatase activity |
| GO:0036222; Molecular function:XTP diphosphatase activity |
| GO:0009146; Biological process:purine nucleoside triphosphate catabolic process |
Cross-references
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Features
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| From: IXTPA_PYRHO (O59580) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 7 | 12 | /ligand="substrate" | [TS]-x-[NSH]-x-x-K | ||||||||
| BINDING | 140 | 143 | /ligand="substrate" | [FWH]-x-x-[DN] | ||||||||
| BINDING | 168 | 169 | /ligand="substrate" | H-R | ||||||||
| ACT_SITE | 65 | 65 | /note="Proton acceptor" | D | ||||||||
| BINDING | 36 | 36 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[ED] | ||||||||
| BINDING | 65 | 65 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 66 | 66 | /ligand="substrate" | [STA] | ||||||||
| BINDING | 163 | 163 | /ligand="substrate" | K | ||||||||
Additional information
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| Size range | 178-328 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_00564 (rph); <Unknown> Cter: None |
| Comments | Unknown N-terminal domain in STRA3, STRA5, STRP1, STRP6, STRP8, STRT1, STRT2 |