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HAMAP rule MF_01414

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General rule information [?]

Accession MF_01414
Dates 2-OCT-2003 (Created)
19-NOV-2022 (Last updated, Version 16)
Name K_H_efflux_KefF
Bacteria; Gammaproteobacteria
Template P0A754 (KEFF_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefF;
AltName: Full=Quinone oxidoreductase KefF;
Gene name

Comments [?]

Function Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC.
Catalytic activity RHEA:46160: a quinone + H(+) + NADH = a quinol + NAD(+)
RHEA:46164: a quinone + H(+) + NADPH = a quinol + NADP(+)
Cofactor FMN
Subunit Homodimer. Interacts with KefC.
Subcellular location Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Similarity Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003955; Molecular function: NAD(P)H dehydrogenase (quinone) activity.
GO:0010181; Molecular function: FMN binding.
GO:0032414; Biological process: positive regulation of ion transmembrane transporter activity.
GO:1901381; Biological process: positive regulation of potassium ion transmembrane transport.

Cross-references [?]

Pfam PF02525; Flavodoxin_2; 1;

Features [?]

From: KEFF_ECOLI (P0A754)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     14     17       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     S-x-x-N  
BINDING (Optional)     65     68       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [ML]-x-W-Y  
BINDING (Optional)     105     108       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     T-x-G-x  
BINDING (Optional)     8     8       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     H  

Additional information [?]

Size range 160-200 amino acids
Related rules None
Fusion None
Comments KefF is the ancillary protein of KefC, whereas KefG is the ancillary protein of KefB. KefF and KefG cannot complement each other and are specific for their respective efflux system