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HAMAP rule MF_01414

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General rule information [?]

Accession MF_01414
Dates 2-OCT-2003 (Created)
19-NOV-2022 (Last updated, Version 16)
Name K_H_efflux_KefF
Scope(s) Bacteria
Template(s) P0A754 (KEFF_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01414 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KEFF
Protein name RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefF;
AltName: Full=Quinone oxidoreductase KefF;
Gene name Name=kefF;

Comments [?]

FUNCTIONRegulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC.
CATALYTIC ACTIVITY Reaction=a quinone + H(+) + NADH = a quinol + NAD(+); Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=;
CATALYTIC ACTIVITY Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+); Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=;
SUBUNITHomodimer. Interacts with KefC.
SUBCELLULAR LOCATIONCell inner membrane; Peripheral membrane protein; Cytoplasmic side.
SIMILARITYBelongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003955; Molecular function:NAD(P)H dehydrogenase (quinone) activity
GO:0010181; Molecular function:FMN binding
GO:0032414; Biological process:positive regulation of ion transmembrane transporter activity
GO:1901381; Biological process:positive regulation of potassium ion transmembrane transport

Cross-references [?]

Pfam PF02525; Flavodoxin_2; 1;

Features [?]

From: KEFF_ECOLI (P0A754)
Key From To Description Tag Condition FTGroup
BINDING 14 17 /ligand="FMN"
BINDING 65 68 /ligand="FMN"
BINDING 105 108 /ligand="FMN"
BINDING 8 8 /ligand="FMN"

Additional information [?]

Size range 160-200 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments KefF is the ancillary protein of KefC, whereas KefG is the ancillary protein of KefB. KefF and KefG cannot complement each other and are specific for their respective efflux system

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