HAMAP rule MF_01414
General rule information
[?]
| Accession | MF_01414 |
| Dates | 2-OCT-2003 (Created)
2-SEP-2024 (Last updated, Version 17) |
| Name | K_H_efflux_KefF |
| Scope(s) |
Bacteria Gammaproteobacteria |
| Template(s) | P0A754 (KEFF_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01414 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | KEFF |
| Protein name | RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefF; AltName: Full=Quinone oxidoreductase KefF; EC=1.6.5.2; |
| Gene name | Name=kefF; |
Comments
[?]
| FUNCTION | Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC. |
| CATALYTIC ACTIVITY | Reaction=a quinone + NADH + H(+) = a quinol + NAD(+); Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2; |
| CATALYTIC ACTIVITY | Reaction=a quinone + NADPH + H(+) = a quinol + NADP(+); Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
| SUBUNIT | Homodimer. Interacts with KefC. |
| SUBCELLULAR LOCATION | Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. |
| SIMILARITY | Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily. |
Keywords
[?]
Gene Ontology
[?]
| GO:0003955; Molecular function:NAD(P)H dehydrogenase (quinone) activity |
| GO:0010181; Molecular function:FMN binding |
| GO:0032414; Biological process:positive regulation of ion transmembrane transporter activity |
| GO:1901381; Biological process:positive regulation of potassium ion transmembrane transport |
Cross-references
[?]
| Pfam | PF02525; Flavodoxin_2; 1; |
Features
[?]
| From: KEFF_ECOLI (P0A754) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 14 | 17 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S-x-x-N | ||||||||
| BINDING | 65 | 68 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[ML]-x-W-Y | ||||||||
| BINDING | 105 | 108 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
T-x-G-x | ||||||||
| BINDING | 8 | 8 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
H | ||||||||
Additional information
[?]
| Size range | 160-200 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | KefF is the ancillary protein of KefC, whereas KefG is the ancillary protein of KefB. KefF and KefG cannot complement each other and are specific for their respective efflux system |