HAMAP rule MF_01414
General rule information
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| Accession | MF_01414 |
| Dates | 2-OCT-2003 (Created) 19-NOV-2022 (Last updated, Version 16) |
| Name | K_H_efflux_KefF |
| Scope | Bacteria; Gammaproteobacteria |
| Template | P0A754 (KEFF_ECOLI) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Regulatory subunit of a potassium efflux system that confers protection against electrophiles. Required for full activity of KefC. Shows redox enzymatic activity, but this enzymatic activity is not required for activation of KefC. |
| Catalytic activity | RHEA:46160: a quinone + H(+) + NADH = a quinol + NAD(+)
EC 1.6.5.2 |
| RHEA:46164: a quinone + H(+) + NADPH = a quinol + NADP(+)
EC 1.6.5.2 |
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| Cofactor | FMN |
| Subunit | Homodimer. Interacts with KefC. |
| Subcellular location | Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. |
| Similarity | Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily. |
Keywords
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Gene Ontology
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GO:0003955; Molecular function: NAD(P)H dehydrogenase (quinone) activity.
GO:0010181; Molecular function: FMN binding.
GO:0032414; Biological process: positive regulation of ion transmembrane transporter activity.
GO:1901381; Biological process: positive regulation of potassium ion transmembrane transport.
GO:0010181; Molecular function: FMN binding.
GO:0032414; Biological process: positive regulation of ion transmembrane transporter activity.
GO:1901381; Biological process: positive regulation of potassium ion transmembrane transport.
Cross-references
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| Pfam | PF02525; Flavodoxin_2; 1; |
Features
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| From: KEFF_ECOLI (P0A754) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING (Optional) | 14 | 17 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | S-x-x-N | ||||||||
| BINDING (Optional) | 65 | 68 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | [ML]-x-W-Y | ||||||||
| BINDING (Optional) | 105 | 108 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | T-x-G-x | ||||||||
| BINDING (Optional) | 8 | 8 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | H | ||||||||
Additional information
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| Size range | 160-200 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | KefF is the ancillary protein of KefC, whereas KefG is the ancillary protein of KefB. KefF and KefG cannot complement each other and are specific for their respective efflux system |