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Annotation rule MF_01428
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General rule information [?]

Accession MF_01428
Dates 14-SEP-2005 (Created)
19-NOV-2019 (Last updated, Version 18)
Name Glu_Q_tRNA_synth
Scope
Bacteria
Template P27305 (GLUQ_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLUQ
Protein name
RecName: Full=Glutamyl-Q tRNA(Asp) synthetase;
Short=Glu-Q-RSs;
EC 6.1.1.-;
Gene name
gluQ

Comments [?]

Function Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon.
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0004812; Molecular function: aminoacyl-tRNA ligase activity.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
Pfam PF00749; tRNA-synt_1c; 1;
PRINTS PR00987; TRNASYNTHGLU; 1;
TIGRFAMs TIGR03838; queuosine_YadB; 1;

Features [?]

From: GLUQ_ECOLI (P27305)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     19     23       Glutamate binding     R-[FLY]-[AS]-P-[ST]  
MOTIF     22     32       'HIGH' region        
MOTIF     238     242       'KMSKS' region        
METAL     111     111       Zinc     C   1
METAL     113     113       Zinc     C   1
METAL     125     125       Zinc     Y   1
METAL     129     129       Zinc     C   1
BINDING (Optional)     55     55       Glutamate     [ED]  
BINDING (Optional)     182     182       Glutamate     Y  
BINDING (Optional)     200     200       Glutamate     R  
BINDING     241     241       ATP     K  

Additional information [?]

Size range 283-347 amino acids
Related rules MF_00022 (SYE supersedes the current rule)
Fusion None
Comments See: PubMed=16164993; Blaise M., Becker H.D., Lapointe J., Cambillau C., Giege R., Kern D.; "Glu-Q-tRNA(Asp) synthetase coded by the yadB gene, a new paralog of aminoacyl-tRNA synthetase that glutamylates tRNA(Asp) anticodon."; Biochimie 87:847-861(2005).