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HAMAP rule MF_01428

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General rule information [?]

Accession MF_01428
Dates 14-SEP-2005 (Created)
1-JUN-2023 (Last updated, Version 22)
Name Glu_Q_tRNA_synth
Scope(s) Bacteria
Template(s) P27305 (GLUQ_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01428 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GLUQ
Protein name RecName: Full=Glutamyl-Q tRNA(Asp) synthetase;
Gene name Name=gluQ;

Comments [?]

FUNCTIONCatalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon.
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004812; Molecular function:aminoacyl-tRNA ligase activity
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

Pfam PF00749; tRNA-synt_1c; 1;
NCBIfam TIGR03838; queuosine_YadB; 1;

Features [?]

From: GLUQ_ECOLI (P27305)
Key From To Description Tag Condition FTGroup
BINDING 19 23 /ligand="L-glutamate"
MOTIF 22 32 /note="'HIGH' region"
MOTIF 238 242 /note="'KMSKS' region"
BINDING 111 111 /ligand="Zn(2+)"
C 1
BINDING 113 113 /ligand="Zn(2+)"
C 1
BINDING 125 125 /ligand="Zn(2+)"
Y 1
BINDING 129 129 /ligand="Zn(2+)"
C 1
BINDING 55 55 /ligand="L-glutamate"
BINDING 182 182 /ligand="L-glutamate"
BINDING 200 200 /ligand="L-glutamate"
BINDING 241 241 /ligand="ATP"

Additional information [?]

Size range 283-347 amino acids
Related rules MF_00022
Fusion Nter: None Cter: None
Comments See: PubMed=16164993; Blaise M., Becker H.D., Lapointe J., Cambillau C., Giege R., Kern D.; "Glu-Q-tRNA(Asp) synthetase coded by the yadB gene, a new paralog of aminoacyl-tRNA synthetase that glutamylates tRNA(Asp) anticodon."; Biochimie 87:847-861(2005).

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