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Annotation rule MF_01438
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General rule information [?]

Accession MF_01438
Dates 7-JUN-2006 (Created)
19-NOV-2019 (Last updated, Version 17)
Name DisA
Scope
Bacteria; Actinobacteria
Bacteria; Bacillaceae
Bacteria; Clostridia
Bacteria; Fusobacterium
Bacteria; Thermotoga
Templates P37573 (DISA_BACSU); Q9WY43 (DISA_THEMA); P9WNW5 (DISA_MYCTU); A0R564 (DISA_MYCS2); Q9X8L6 (DISA_STRCO); A0R8F5 (DISA_BACAH); Q6HPT4 (DISA_BACHK): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DISA
Protein name
RecName: Full=DNA integrity scanning protein DisA;
AltName: Full=Diadenylate cyclase;
EC 2.7.7.85;
AltName: Full=Cyclic di-AMP synthase;
Short=c-di-AMP synthase;
Gene name
disA

Comments [?]

case <OC:Bacillaceae> or <OC:Clostridia> or <OC:Frankia> or <OC:Streptomyces>
Function Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. DisA forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation.
Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome, operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis.
else
Function Participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions.
Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP likely acts as a signaling molecule that may couple DNA integrity with a cellular process.
end case
Catalytic activity RHEA:35655: 2 ATP = 3',3'-c-di-AMP + 2 diphosphate
EC 2.7.7.85
Cofactor Mg(2+)
Subunit Homooctamer.
Similarity Belongs to the DisA family.

Keywords [?]


Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0005524; Molecular function: ATP binding.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0006281; Biological process: DNA repair.
GO:0019932; Biological process: second-messenger-mediated signaling.

Cross-references [?]

PROSITE PS51794; DAC; 1; trigger=PRU01130;
Pfam PF10635; DisA-linker; 1;
PF02457; DisA_N; 1;
PF00633; HHH; 1;

Features [?]

From: DISA_THEMA (Q9WY43)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     107     111       ATP     x-R-x-x-[TS]  
BINDING     76     76       ATP; via amide nitrogen     G  
BINDING     94     94       ATP; via amide nitrogen and carbonyl oxygen     [LV]  

Additional information [?]

Size range 344-374 amino acids
Related rules MF_00838 (DACB); MF_00840 (DACZ); MF_01499 (DACA)
Fusion None