HAMAP rule MF_01446
General rule information
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Accession | MF_01446 |
Dates | 13-AUG-2007 (Created) 19-NOV-2022 (Last updated, Version 22) |
Name | Kae1 |
Scope | Archaea |
Templates | Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST): [Recover all] |
case <OC:Archaea>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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case <OC:Euryarchaeota>
Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. |
Subunit | Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes. |
else
Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. |
end case
Catalytic activity | RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234 |
Cofactor | Fe(2+) Note: Binds 1 Fe(2+) ion per subunit. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the KAE1 / TsaD family. |
Keywords
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Gene Ontology
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GO:0005506; Molecular function: iron ion binding.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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PROSITE | PS01016; GLYCOPROTEASE; 1; |
Pfam | PF00814; Peptidase_M22; 1; |
PRINTS | PR00789; OSIALOPTASE; 1; |
TIGRFAMs | TIGR03722; Arch_KAE1; 1; |
TIGR00329; Gcp_kae1; 1; |
Features
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From: KAE1_PYRAB (Q9UXT7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 127 | 131 | /ligand="substrate | x-x-[SA]-G-[GA] | ||||||||
BINDING | 107 | 107 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | ||||||||
BINDING | 111 | 111 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | ||||||||
BINDING (Optional) | 127 | 127 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | Y | ||||||||
BINDING | 285 | 285 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | D | ||||||||
BINDING | 159 | 159 | /ligand="substrate | D | ||||||||
BINDING (Optional) | 172 | 172 | /ligand="substrate | G | ||||||||
BINDING | 176 | 176 | /ligand="substrate | [ED] | ||||||||
BINDING | 257 | 257 | /ligand="substrate | [NS] |
Additional information
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Size range | 314-363 amino acids |
Related rules | MF_01447 (KAE1B supersedes the current rule) |
Fusion | None |
Comments | Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954). Some Archaea contain a kinase domain in the C-terminus. These sequences are represented in the MF_01447 family rule. |