![]() |
|
Annotation rule MF_01446 |
Accession | MF_01446 |
Dates | 13-AUG-2007 (Created) 19-NOV-2019 (Last updated, Version 18) |
Name | Kae1 |
Scope | Archaea |
Templates | Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST): [Recover all] |
Triggered by |
Identifier |
|
Protein name |
|
Gene name |
|
Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. |
Subunit | Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes. |
Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. |
Catalytic activity | RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234 |
Cofactor | Fe(2+) Note: Binds 1 Fe(2+) ion per subunit. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the KAE1 / TsaD family. |
PROSITE | PS01016; GLYCOPROTEASE; 1; |
Pfam | PF00814; Peptidase_M22; 1; |
PRINTS | PR00789; OSIALOPTASE; 1; |
TIGRFAMs | TIGR03722; Arch_KAE1; 1; |
TIGR00329; Gcp_kae1; 1; |
From: KAE1_PYRAB (Q9UXT7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 127 | 131 | Substrate binding | x-x-[SA]-G-[GA] | ||||||||
METAL | 107 | 107 | Iron | H | ||||||||
METAL | 111 | 111 | Iron | H | ||||||||
METAL (Optional) | 127 | 127 | Iron | Y | ||||||||
METAL | 285 | 285 | Iron | D | ||||||||
BINDING | 159 | 159 | Substrate | D | ||||||||
BINDING (Optional) | 172 | 172 | Substrate; via amide nitrogen | G | ||||||||
BINDING | 176 | 176 | Substrate | [ED] | ||||||||
BINDING | 257 | 257 | Substrate | [NS] |
Size range | 314-363 amino acids |
Related rules | MF_01447 (KAE1B supersedes the current rule) |
Fusion | None |
Comments | Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954). Some Archaea contain a kinase domain in the C-terminus. These sequences are represented in the MF_01447 family rule. |