HAMAP rule MF_01446

General rule information [?]

Accession	MF_01446
Dates	13-AUG-2007 (Created) 19-NOV-2022 (Last updated, Version 22)

Name

Kae1

Scope

Archaea

Templates

Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST): [Recover all]

case <OC:Archaea>

Triggered by

HAMAP; MF_01446 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

KAE1

Protein name

RecName:		Full=tRNA N6-adenosine threonylcarbamoyltransferase;
		EC 2.3.1.234;
AltName:		Full=N6-L-threonylcarbamoyladenine synthase;
		Short=t(6)A synthase;
AltName:		Full=tRNA threonylcarbamoyladenosine biosynthesis protein Kae1;
AltName:		Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1;

Gene name

kae1

Comments [?]

case <OC:Euryarchaeota>

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.

Subunit

Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.

else

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.

end case

Catalytic activity	RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA EC 2.3.1.234
Cofactor	Fe(2+) Note: Binds 1 Fe(2+) ion per subunit.
Subcellular location	Cytoplasm.
Similarity	Belongs to the KAE1 / TsaD family.

Keywords [?]

Acyltransferase
Cytoplasm
Iron
Metal-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS01016; GLYCOPROTEASE; 1;
Pfam	PF00814; Peptidase_M22; 1;
PRINTS	PR00789; OSIALOPTASE; 1;
TIGRFAMs	TIGR03722; Arch_KAE1; 1;
	TIGR00329; Gcp_kae1; 1;

Features [?]

From: KAE1_PYRAB (Q9UXT7)

Key From To Description Tag Condition FTGroup

BINDING 127 131 /ligand="substrate x-x-[SA]-G-[GA]

BINDING 107 107 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H

BINDING 111 111 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 H

BINDING (Optional) 127 127 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 Y

BINDING 285 285 /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 D

BINDING 159 159 /ligand="substrate D

BINDING (Optional) 172 172 /ligand="substrate G

BINDING 176 176 /ligand="substrate [ED]

BINDING 257 257 /ligand="substrate [NS]

Additional information [?]

Size range	314-363 amino acids
Related rules	MF_01447 (KAE1B supersedes the current rule)
Fusion	None
Comments	Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954). Some Archaea contain a kinase domain in the C-terminus. These sequences are represented in the MF_01447 family rule.

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