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Annotation rule MF_01447 |
Accession | MF_01447 |
Dates | 13-AUG-2007 (Created) 19-NOV-2022 (Last updated, Version 22) |
Name | Kae1_Bud32_arch |
Scope | Archaea; Euryarchaeota |
Templates | Q58530 (KAE1B_METJA); P36132 (KAE1_YEAST): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function. |
Catalytic activity | RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234 |
RHEA:17989: ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
EC 2.7.11.1 |
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RHEA:46608: ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
EC 2.7.11.1 |
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Cofactor | Fe(2+) Note: Binds 1 Fe(2+) ion per subunit. |
Subunit | Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes. |
Subcellular location | Cytoplasm. |
Similarity | In the N-terminal section; belongs to the KAE1 / TsaD family. |
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. |
PROSITE | PS01016; GLYCOPROTEASE; 1; |
PS00107; PROTEIN_KINASE_ATP; 1; | |
PS50011; PROTEIN_KINASE_DOM; 1; | |
PS00109; PROTEIN_KINASE_TYR; 1; | |
Pfam | PF00814; Peptidase_M22; 1; |
PF00069; Pkinase; 1; | |
PF01163; RIO1; 1; | |
PRINTS | PR00789; OSIALOPTASE; 1; |
TIGRFAMs | TIGR03724; Arch_bud32; 1; |
TIGR03722; Arch_KAE1; 1; | |
TIGR00329; Gcp_kae1; 1; | |
PIRSF | PIRSF036401; Gcp_STYKS; 1; |
From: KAE1B_METJA (Q58530) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | 333 | Cter | Protein kinase | |||||||||
BINDING | 339 | 347 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | |||||||||
REGION | Nter | 323 | Kae1 | |||||||||
ACT_SITE | 451 | 451 | Proton acceptor; for kinase activity | D | ||||||||
BINDING | 127 | 131 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682 | x-x-S-G-[GA] | ||||||||
BINDING | 106 | 106 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | ||||||||
BINDING | 110 | 110 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | H | ||||||||
BINDING (Optional) | 127 | 127 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | Y | ||||||||
BINDING | 284 | 284 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875 | D | ||||||||
BINDING | 159 | 159 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682 | D | ||||||||
BINDING | 172 | 172 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682 | G | ||||||||
BINDING | 176 | 176 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682 | E | ||||||||
BINDING | 256 | 256 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682 | N | ||||||||
BINDING | 360 | 360 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K |
Size range | 520-548 amino acids |
Related rules | MF_01446 (KAE1) |
Fusion | None |