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Annotation rule MF_01447
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General rule information [?]

Accession MF_01447
Dates 13-AUG-2007 (Created)
19-NOV-2022 (Last updated, Version 22)
Name Kae1_Bud32_arch
Scope
Archaea; Euryarchaeota
Templates Q58530 (KAE1B_METJA); P36132 (KAE1_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
KAE1B
Protein name
RecName: Full=Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein;
Includes:
RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase;
EC 2.3.1.234;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein Kae1;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1;
Includes:
RecName: Full=Serine/threonine-protein kinase Bud32;
EC 2.7.11.1;

Comments [?]

Function Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.
Catalytic activity RHEA:37059: adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA
EC 2.3.1.234
RHEA:17989: ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
EC 2.7.11.1
RHEA:46608: ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
EC 2.7.11.1
Cofactor Fe(2+)
Note: Binds 1 Fe(2+) ion per subunit.
Subunit Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Keywords [?]

Acyltransferase
ATP-binding
Cytoplasm
Kinase
Nucleotide-binding
Serine/threonine-protein kinase
Transferase
tRNA processing
Iron
Metal-binding
Multifunctional enzyme

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004712; Molecular function: protein serine/threonine/tyrosine kinase activity.
GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01016; GLYCOPROTEASE; 1;
PS00107; PROTEIN_KINASE_ATP; 1;
PS50011; PROTEIN_KINASE_DOM; 1;
PS00109; PROTEIN_KINASE_TYR; 1;
Pfam PF00814; Peptidase_M22; 1;
PF00069; Pkinase; 1;
PF01163; RIO1; 1;
PRINTS PR00789; OSIALOPTASE; 1;
TIGRFAMs TIGR03724; Arch_bud32; 1;
TIGR03722; Arch_KAE1; 1;
TIGR00329; Gcp_kae1; 1;
PIRSF PIRSF036401; Gcp_STYKS; 1;

Features [?]

From: KAE1B_METJA (Q58530)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     333     Cter       Protein kinase        
BINDING     339     347       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        
REGION     Nter     323       Kae1        
ACT_SITE     451     451       Proton acceptor; for kinase activity     D  
BINDING     127     131       /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682     x-x-S-G-[GA]  
BINDING     106     106       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     H  
BINDING     110     110       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     H  
BINDING (Optional)     127     127       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     Y  
BINDING     284     284       /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875     D  
BINDING     159     159       /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682     D  
BINDING     172     172       /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682     G  
BINDING     176     176       /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682     E  
BINDING     256     256       /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682     N  
BINDING     360     360       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  

Additional information [?]

Size range 520-548 amino acids
Related rules MF_01446 (KAE1)
Fusion None


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