HAMAP rule MF_01451

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01451
Accession	MF_01451
Dates	04-JUN-2009 (Created) 02-SEP-2024 (Last updated, Version 19)
Name	AddA
Scope(s)	Bacteria Bacillota
Template(s)	P23478; A2RH77; Q97QP9; [ Recover all ]
Triggered by	HAMAP; MF_01451 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	ADDA
Protein name	RecName: Full=ATP-dependent helicase/nuclease subunit A; EC=3.1.-.-; EC=5.6.2.4; AltName: Full=ATP-dependent helicase/nuclease AddA; AltName: Full=DNA 3'-5' helicase AddA;
Gene name	Name=addA;

Comments [?]

FUNCTION	The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
CATALYTIC ACTIVITY	Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.; EC=5.6.2.4;
CATALYTIC ACTIVITY	Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNIT	Heterodimer of AddA and AddB/RexB.
SIMILARITY	Belongs to the helicase family. AddA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003678; Molecular function:DNA helicase activity

GO:0005524; Molecular function:ATP binding

GO:0043138; Molecular function:3'-5' DNA helicase activity

GO:0008408; Molecular function:3'-5' exonuclease activity

GO:0003690; Molecular function:double-stranded DNA binding

GO:0000724; Biological process:double-strand break repair via homologous recombination

Cross-references [?]

Features [?]

Additional information [?]

Size range	1167-1405 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Originally called rexA in Lactobacillus and Streptococcus. Forms a heterodimer with 1 of 2 related proteins; in Bacillus and Staphylococcus the partner is addB, in Lactobacillus and Streptococcus the partner is rexB.