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HAMAP rule MF_01451

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General rule information [?]

Accession MF_01451
Dates 4-JUN-2009 (Created)
13-NOV-2023 (Last updated, Version 17)
Name AddA
Scope(s) Bacteria
Bacillota
Template(s) P23478 (ADDA_BACSU); A2RH77 (ADDA_LACLM); Q97QP9 (ADDA_STRPN); [ Recover all ]
Triggered by HAMAP; MF_01451 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ADDA
RecName: Full=ATP-dependent helicase/nuclease subunit A;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/5.6.2.4">5.6.2.4</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease AddA;<br /> AltName: Full=DNA 3'-5' helicase AddA;
Gene name Name=addA;

Comments [?]

FUNCTIONThe heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
CATALYTIC ACTIVITY Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.; EC=5.6.2.4;
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNITHeterodimer of AddA and AddB/RexB.
SIMILARITYBelongs to the helicase family. AddA subfamily.

Keywords [?]

ATP-binding
DNA damage
DNA repair
DNA-binding
Exonuclease
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Hydrolase
Isomerase
Nuclease
Nucleotide-binding

Gene Ontology [?]

GO:0003678; Molecular function:DNA helicase activity
GO:0005524; Molecular function:ATP binding
GO:0043138; Molecular function:3'-5' DNA helicase activity
GO:0008408; Molecular function:3'-5' exonuclease activity
GO:0003690; Molecular function:double-stranded DNA binding
GO:0000724; Biological process:double-strand break repair via homologous recombination

Cross-references [?]

PROSITE PS51198; UVRD_HELICASE_ATP_BIND; 1;
PROSITE PS51217; UVRD_HELICASE_CTER; 1;
Pfam PF00580; UvrD-helicase; 1;
NCBIfam TIGR02785; AddA_Gpos; 1;

Features [?]


Additional information [?]

Size range 1167-1405 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Originally called rexA in Lactobacillus and Streptococcus. Forms a heterodimer with 1 of 2 related proteins; in Bacillus and Staphylococcus the partner is addB, in Lactobacillus and Streptococcus the partner is rexB.



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