HAMAP rule MF_01451
General rule information
[?]
Accession | MF_01451 |
Dates | 4-JUN-2009 (Created)
13-NOV-2023 (Last updated, Version 17) |
Name | AddA |
Scope(s) |
Bacteria Bacillota |
Template(s) | P23478 (ADDA_BACSU); A2RH77 (ADDA_LACLM); Q97QP9 (ADDA_STRPN); [ Recover all ] |
Triggered by |
HAMAP; MF_01451 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier | ADDA |
RecName: Full=ATP-dependent helicase/nuclease subunit A;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> EC=<a href="https://enzyme.expasy.org/EC/5.6.2.4">5.6.2.4</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease AddA;<br /> AltName: Full=DNA 3'-5' helicase AddA; | |
Gene name | Name=addA; |
Comments
[?]
FUNCTION | The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities. |
CATALYTIC ACTIVITY | Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.; EC=5.6.2.4; |
CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
SUBUNIT | Heterodimer of AddA and AddB/RexB. |
SIMILARITY | Belongs to the helicase family. AddA subfamily. |
Keywords
[?]
ATP-binding | |
DNA damage | |
DNA repair | |
DNA-binding | |
Exonuclease | |
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
Hydrolase | |
Isomerase | |
Nuclease | |
Nucleotide-binding |
Gene Ontology
[?]
GO:0003678; Molecular function:DNA helicase activity |
GO:0005524; Molecular function:ATP binding |
GO:0043138; Molecular function:3'-5' DNA helicase activity |
GO:0008408; Molecular function:3'-5' exonuclease activity |
GO:0003690; Molecular function:double-stranded DNA binding |
GO:0000724; Biological process:double-strand break repair via homologous recombination |
Cross-references
[?]
PROSITE | PS51198; UVRD_HELICASE_ATP_BIND; 1; |
PROSITE | PS51217; UVRD_HELICASE_CTER; 1; |
Pfam | PF00580; UvrD-helicase; 1; |
NCBIfam | TIGR02785; AddA_Gpos; 1; |
Features
[?]
Additional information
[?]
Size range | 1167-1405 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |
Comments | Originally called rexA in Lactobacillus and Streptococcus. Forms a heterodimer with 1 of 2 related proteins; in Bacillus and Staphylococcus the partner is addB, in Lactobacillus and Streptococcus the partner is rexB. |