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Annotation rule MF_01452
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General rule information [?]

Accession MF_01452
Dates 4-JUN-2009 (Created)
19-NOV-2019 (Last updated, Version 13)
Name AddB_type1
Scope
Bacteria; Firmicutes
Template P23477 (ADDB_BACSU)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ADDB
Protein name
RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;
EC 3.1.-.-;
EC 3.6.4.12;
AltName: Full=ATP-dependent helicase/nuclease AddB;
Gene name
addB

Comments [?]

Function The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity.
Catalytic activity RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
EC 3.6.4.12
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
end case
Cofactor Mg(2+)
Subunit Heterodimer of AddA and AddB.
Similarity Belongs to the helicase family. AddB/RexB type 1 subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0008409; Molecular function: 5'-3' exonuclease activity.
GO:0003690; Molecular function: double-stranded DNA binding.
case <FTGroup:1>
GO:0051536; Molecular function: iron-sulfur cluster binding.
end case
GO:0000724; Biological process: double-strand break repair via homologous recombination.

Cross-references [?]

PROSITE PS51198; UVRD_HELICASE_ATP_BIND; 1; trigger=PRU00560;
PS51217; UVRD_HELICASE_CTER; 1; trigger=PRU00617;
Pfam PF00580; UvrD-helicase; 1;
PF04257; Exonuc_V_gamma; 1;
TIGRFAMs TIGR02773; AddB_Gpos; 1;

Features [?]

From: ADDB_BACSU (P23477)
Key     From     To       Description   Tag   Condition   FTGroup
METAL (Optional)     801     801       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1121     1121       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1124     1124       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1130     1130       Iron-sulfur (4Fe-4S)     C   1

Additional information [?]

Size range 1134-1224 amino acids
Related rules MF_01453 (ADDB)
Fusion None
Comments Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (this family), and rexB in Lactobacillus and Streptococcus (MF_01453). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low-iron environment. Not all members match the uvrD_helicase profiles (PS51198 and PS51217).