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HAMAP rule MF_01452
General rule information
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| Accession | MF_01452 |
| Dates | 4-JUN-2009 (Created)
10-NOV-2023 (Last updated, Version 20) |
| Name | AddB_type1 |
| Scope(s) |
Bacteria Bacillota |
| Template(s) | P23477 (ADDB_BACSU); [ Recover all ] |
| Triggered by |
HAMAP; MF_01452 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ADDB |
| RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease subunit AddB; | |
| Gene name | Name=addB; |
Comments
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| FUNCTION | The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB subunit has 5' -> 3' nuclease activity but not helicase activity. |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.; |
| end case | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Heterodimer of AddA and AddB. |
| MISCELLANEOUS | Despite having conserved helicase domains, this subunit does not have helicase activity. |
| SIMILARITY | Belongs to the helicase family. AddB/RexB type 1 subfamily. |
Keywords
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| ATP-binding | |
| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Exonuclease | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Hydrolase | |
| Nuclease | |
| Nucleotide-binding | |
| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding | |
| GO:0008409; Molecular function:5'-3' exonuclease activity | |
| GO:0003690; Molecular function:double-stranded DNA binding | |
| case <FTGroup:1> | |
| GO:0051536; Molecular function:iron-sulfur cluster binding | |
| end case | |
| GO:0000724; Biological process:double-strand break repair via homologous recombination | |
Cross-references
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| Pfam | PF21445; ADDB_N; 1; |
| Pfam | PF12705; PDDEXK_1; 1; |
| PROSITE | PS51198; UVRD_HELICASE_ATP_BIND; 0-1; |
| PROSITE | PS51217; UVRD_HELICASE_CTER; 0-1; |
| NCBIfam | TIGR02773; AddB_Gpos; 1; |
Features
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| From: ADDB_BACSU (P23477) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 801 | 801 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1121 | 1121 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1124 | 1124 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1130 | 1130 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
Additional information
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| Size range | 1134-1224 amino acids |
| Related rules |
MF_01453 |
| Fusion | Nter: None Cter: None |
| Comments | Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (this family), and rexB in Lactobacillus and Streptococcus (MF_01453). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low-iron environment. Not all members match the uvrD_helicase profiles (PS51198 and PS51217). |