HAMAP rule MF_01472
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01472 |
| Accession | MF_01472 |
| Dates | 14-JUN-2012 (Created)
02-SEP-2024 (Last updated, Version 17) |
| Name | GtfA |
| Scope(s) |
Bacteria Bacillota |
| Template(s) | A0A0H2URG7; A0A0S4NM89; A1C3L9; Q3S2Y2; Q9AET5; [ Recover all ] |
| Triggered by |
HAMAP; MF_01472 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GTFA |
| Protein name | RecName: Full=UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit; EC=2.4.1.-; AltName: Full=Glycosyltransferase GtfA; |
| Gene name | Name=gtfA; |
Comments
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| FUNCTION | Required for polymorphic O-glycosylation of the serine-rich repeat protein in this bacteria. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in the substrate protein. Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins usually encoded upstream in the same operon. |
| CATALYTIC ACTIVITY | Reaction=L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N- acetyl-alpha-D-glucosaminyl]-L-seryl-[protein] + UDP + H(+); Xref=Rhea:RHEA:59872, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:15471, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:143279; |
| PATHWAY | Protein modification; protein glycosylation. |
| SUBUNIT | Forms a heterotetramer with 2 subunits each of GtfA and GtfB. Part of the accessory SecA2/SecY2 protein translocation apparatus. |
| SUBCELLULAR LOCATION | Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Cell membrane association requires GtfB. |
| SIMILARITY | Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. |
Keywords
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Gene Ontology
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| GO:0018242; Biological process:protein O-linked glycosylation via serine |
| GO:0016757; Molecular function:glycosyltransferase activity |
| GO:0017122; Cellular component:protein N-acetylglucosaminyltransferase complex |
| GO:0005886; Cellular component:plasma membrane |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: GTFA_STRPN (A0A0H2URG7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 16 | 19 | /ligand="UDP" /ligand_id="ChEBI:CHEBI:58223" |
G-V-E-Y | ||||||||
| BINDING | 384 | 385 | /ligand="UDP" /ligand_id="ChEBI:CHEBI:58223" |
[HQ]-[AHKQRVY] | ||||||||
| BINDING | 404 | 407 | /ligand="N-acetyl-D-glucosamine" /ligand_id="ChEBI:CHEBI:506227" |
E-G-F-G | ||||||||
| BINDING | 242 | 242 | /ligand="N-acetyl-D-glucosamine" /ligand_id="ChEBI:CHEBI:506227" |
H | ||||||||
Additional information
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| Size range | 490-548 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |