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HAMAP rule MF_01472

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General rule information [?]

Accession MF_01472
Dates 14-JUN-2012 (Created)
1-JUN-2023 (Last updated, Version 15)
Name GtfA
Scope(s) Bacteria
Template(s) A0A0H2URG7 (GTFA_STRPN); A0A0S4NM89 (GTFA_LIMR5); A1C3L9 (GTFA_STRPA); Q3S2Y2 (GTFA_STRAG); Q9AET5 (GTFA_STRGN); [ Recover all ]
Triggered by HAMAP; MF_01472 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GTFA
Protein name RecName: Full=UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit;
AltName: Full=Glycosyltransferase GtfA;
Gene name Name=gtfA;

Comments [?]

FUNCTIONRequired for polymorphic O-glycosylation of the serine-rich repeat protein in this bacteria. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in the substrate protein. Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins usually encoded upstream in the same operon.
CATALYTIC ACTIVITY Reaction=L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N- acetyl-alpha-D-glucosaminyl]-L-seryl-[protein] + H(+) + UDP; Xref=Rhea:RHEA:59872, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:15471, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:143279;
PATHWAYProtein modification; protein glycosylation.
SUBUNITForms a heterotetramer with 2 subunits each of GtfA and GtfB. Part of the accessory SecA2/SecY2 protein translocation apparatus.
SUBCELLULAR LOCATIONCytoplasm. Cell membrane; Peripheral membrane protein. Note=Cell membrane association requires GtfB.
SIMILARITYBelongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0018242; Biological process:protein O-linked glycosylation via serine
GO:0016757; Molecular function:glycosyltransferase activity
GO:0017122; Cellular component:protein N-acetylglucosaminyltransferase complex
GO:0005886; Cellular component:plasma membrane
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00534; Glycos_transf_1; 1;
NCBIfam TIGR02918; TIGR02918; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 16 19 /ligand="UDP"
BINDING 384 385 /ligand="UDP"
BINDING 404 407 /ligand="N-acetyl-D-glucosamine"
BINDING 242 242 /ligand="N-acetyl-D-glucosamine"

Additional information [?]

Size range 490-548 amino acids
Related rules None
Fusion Nter: None Cter: None

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