Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
HAMAP rule MF_01490
General rule information
[?]
| Accession | MF_01490 |
| Dates | 3-APR-2014 (Created)
11-AUG-2023 (Last updated, Version 13) |
| Name | HJ_Resolv_Hjc |
| Scope(s) |
Archaea |
| Template(s) | Q7LXU0 (HJC_SACS2); O27336 (HJC_METTH); F9VND5 (HJC_SULTO); E7FHX4 (HJC_PYRFU); [ Recover all ] |
| Triggered by |
HAMAP; MF_01490 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | HJC |
| Protein name | RecName: Full=Crossover junction endodeoxyribonuclease Hjc; Short=Hjc; EC=3.1.21.10; AltName: Full=Holliday junction resolvase Hjc; |
| Gene name | Name=hjc; |
Comments
[?]
| FUNCTION | A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the Holliday junction resolvase Hjc family. |
Keywords
[?]
| DNA damage | |
| DNA recombination | |
| DNA repair | |
| DNA-binding | |
| Endonuclease | |
| Hydrolase | |
| case <FTGroup:1> | |
| Metal-binding | |
| Magnesium | |
| end case | |
| Nuclease | |
Gene Ontology
[?]
| GO:0008821; Molecular function:crossover junction DNA endonuclease activity | |
| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0006310; Biological process:DNA recombination | |
| GO:0006281; Biological process:DNA repair | |
Cross-references
[?]
| Pfam | PF01870; Hjc; 1; |
| PIRSF | PIRSF004985; Hlld_jn_rslvs_ar; 1; |
Features
[?]
| From: HJC_SACS2 (Q7LXU0) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 32 | 32 | S | |||||||||
| BINDING | 12 | 12 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 42 | 42 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 55 | 55 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| SITE | 57 | 57 | /note="Transition state stabilizer" | K | ||||||||
Additional information
[?]
| Size range | 108-183 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus. |