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Annotation rule MF_01490
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General rule information [?]

Accession MF_01490
Dates 3-APR-2014 (Created)
19-NOV-2019 (Last updated, Version 7)
Name HJ_Resolv_Hjc
Scope
Archaea
Templates Q7LXU0 (HJC_SACS2); O27336 (HJC_METTH); F9VND5 (HJC_SULTO); E7FHX4 (HJC_PYRFU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HJC
Protein name
RecName: Full=Holliday junction resolvase Hjc;
Short=Hjc;
EC 3.1.22.4;
Gene name
hjc

Comments [?]

Function A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.
Catalytic activity Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.22.4;
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Subunit Homodimer.
Similarity Belongs to the Holliday junction resolvase Hjc family.

Keywords [?]

DNA damage
DNA recombination
DNA repair
DNA-binding
Endonuclease
Hydrolase
case <FTGroup:1>
Metal-binding
Magnesium
end case
Nuclease

Gene Ontology [?]

GO:0008821; Molecular function: crossover junction endodeoxyribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF01870; Hjc; 1;
PIRSF PIRSF004985; Hlld_jn_rslvs_ar; 1;

Features [?]

From: HJC_SACS2 (Q7LXU0)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     32     32             S  
METAL     12     12       Magnesium     E   1
METAL     42     42       Magnesium     D   1
METAL     55     55       Magnesium     E   1
BINDING     57     57       Stablizes the transition state     K  

Additional information [?]

Size range 108-183 amino acids
Related rules None
Fusion None
Comments A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus.


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