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Annotation rule MF_01490 |
General rule information
[?]
Accession |
MF_01490 |
Dates |
3-APR-2014 (Created) 19-NOV-2019 (Last updated, Version 7) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=Holliday junction resolvase Hjc;
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Short=Hjc;
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EC 3.1.22.4;
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Function |
A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. |
Catalytic activity |
Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.22.4; |
case <FTGroup:1>
Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
Subunit |
Homodimer. |
Similarity |
Belongs to the Holliday junction resolvase Hjc family. |
case <FTGroup:1>
end case
GO:0008821; Molecular function: crossover junction endodeoxyribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
From: HJC_SACS2 (Q7LXU0) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ACT_SITE
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32
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32
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S
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METAL
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12
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12
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Magnesium
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E
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1
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METAL
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42
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42
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Magnesium
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D
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1
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METAL
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55
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55
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Magnesium
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E
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1
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BINDING
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57
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57
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Stablizes the transition state
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K
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Additional information
[?]
Size range |
108-183 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus. |