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HAMAP rule MF_01490

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General rule information [?]

Accession MF_01490
Dates 3-APR-2014 (Created)
11-AUG-2023 (Last updated, Version 13)
Name HJ_Resolv_Hjc
Scope(s) Archaea
Template(s) Q7LXU0 (HJC_SACS2); O27336 (HJC_METTH); F9VND5 (HJC_SULTO); E7FHX4 (HJC_PYRFU); [ Recover all ]
Triggered by HAMAP; MF_01490 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HJC
Protein name RecName: Full=Crossover junction endodeoxyribonuclease Hjc;
AltName: Full=Holliday junction resolvase Hjc;
Gene name Name=hjc;

Comments [?]

FUNCTIONA structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.
CATALYTIC ACTIVITY Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
SIMILARITYBelongs to the Holliday junction resolvase Hjc family.

Keywords [?]

Gene Ontology [?]

GO:0008821; Molecular function:crossover junction DNA endonuclease activity
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0006310; Biological process:DNA recombination
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF01870; Hjc; 1;
PIRSF PIRSF004985; Hlld_jn_rslvs_ar; 1;

Features [?]

From: HJC_SACS2 (Q7LXU0)
Key From To Description Tag Condition FTGroup
ACT_SITE 32 32 S
BINDING 12 12 /ligand="Mg(2+)"
E 1
BINDING 42 42 /ligand="Mg(2+)"
D 1
BINDING 55 55 /ligand="Mg(2+)"
E 1
SITE 57 57 /note="Transition state stabilizer" K

Additional information [?]

Size range 108-183 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus.

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