HAMAP rule MF_01490
General rule information
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Accession | MF_01490 |
Dates | 3-APR-2014 (Created) 7-FEB-2023 (Last updated, Version 12) |
Name | HJ_Resolv_Hjc |
Scope | Archaea |
Templates | Q7LXU0 (HJC_SACS2); O27336 (HJC_METTH); F9VND5 (HJC_SULTO); E7FHX4 (HJC_PYRFU): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. |
Catalytic activity | Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10; |
case <FTGroup:1>
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
Subunit | Homodimer. |
Similarity | Belongs to the Holliday junction resolvase Hjc family. |
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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GO:0008821; Molecular function: crossover junction DNA endonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
Cross-references
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Pfam | PF01870; Hjc; 1; |
PIRSF | PIRSF004985; Hlld_jn_rslvs_ar; 1; |
Features
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From: HJC_SACS2 (Q7LXU0) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 32 | 32 | S | |||||||||
BINDING | 12 | 12 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | E | 1 | |||||||
BINDING | 42 | 42 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | 1 | |||||||
BINDING | 55 | 55 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | E | 1 | |||||||
SITE | 57 | 57 | Transition state stabilizer | K |
Additional information
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Size range | 108-183 amino acids |
Related rules | None |
Fusion | None |
Comments | A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus. |