HAMAP rule MF_01490

General rule information [?]

Accession	MF_01490
Dates	3-APR-2014 (Created) 7-FEB-2023 (Last updated, Version 12)

Name	HJ_Resolv_Hjc

Scope

Archaea

Templates

Q7LXU0 (HJC_SACS2); O27336 (HJC_METTH); F9VND5 (HJC_SULTO); E7FHX4 (HJC_PYRFU): [Recover all]

Triggered by

HAMAP; MF_01490 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

HJC

Protein name

RecName:		Full=Holliday junction resolvase Hjc;
		Short=Hjc;
		EC 3.1.21.10;

Gene name

hjc

Comments [?]

Function	A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.
Catalytic activity	Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10;

case <FTGroup:1>

Cofactor

Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.

end case

Subunit	Homodimer.
Similarity	Belongs to the Holliday junction resolvase Hjc family.

Keywords [?]

DNA damage
DNA recombination
DNA repair
DNA-binding
Endonuclease
Hydrolase

case <FTGroup:1>

Metal-binding
Magnesium

end case

Nuclease

Gene Ontology [?]

GO:0008821; Molecular function: crossover junction DNA endonuclease activity.

case <FTGroup:1>

GO:0000287; Molecular function: magnesium ion binding.

end case

GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam	PF01870; Hjc; 1;
PIRSF	PIRSF004985; Hlld_jn_rslvs_ar; 1;

Features [?]

From: HJC_SACS2 (Q7LXU0)

Key From To Description Tag Condition FTGroup

ACT_SITE 32 32 S

BINDING 12 12 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E 1

BINDING 42 42 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D 1

BINDING 55 55 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E 1

SITE 57 57 Transition state stabilizer K

Additional information [?]

Size range	108-183 amino acids
Related rules	None
Fusion	None
Comments	A second, related Holliday juntion resolving enzyme, Hje, with different characteristics exists in some Sulfolobaceae. Another may exist in Pyrococcus.

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