HAMAP rule MF_01540
General rule information
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| Accession | MF_01540 |
| Dates | 22-AUG-2005 (Created)
3-SEP-2024 (Last updated, Version 17) |
| Name | CysI |
| Scope(s) |
Bacteria |
| Template(s) | P17846 (CYSI_ECOLI); P17845 (CYSI_SALTY); O32213 (CYSI_BACSU); Q8KQT8 (CYSI_XANOR); [ Recover all ] |
| Triggered by |
HAMAP; MF_01540 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CYSI |
| Protein name | RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component; Short=SiR-HP; Short=SiRHP; EC=1.8.1.2; |
| Gene name | Name=cysI; |
Comments
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| FUNCTION | Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. |
| CATALYTIC ACTIVITY | Reaction=hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+); Xref=Rhea:RHEA:13801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359, ChEBI:CHEBI:29919, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.2; |
| COFACTOR | Name=siroheme; Xref=ChEBI:CHEBI:60052; Note=Binds 1 siroheme per subunit.; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.; |
| PATHWAY | Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. |
| SUBUNIT | Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein. |
| SIMILARITY | Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. |
Keywords
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| 4Fe-4S |
| Amino-acid biosynthesis |
| Cysteine biosynthesis |
| Heme |
| Iron |
| Iron-sulfur |
| Metal-binding |
| NADP |
| Oxidoreductase |
Gene Ontology
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| GO:0004783; Molecular function:sulfite reductase (NADPH) activity |
| GO:0000103; Biological process:sulfate assimilation |
| GO:0070814; Biological process:hydrogen sulfide biosynthetic process |
Cross-references
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| PROSITE | PS00365; NIR_SIR; 1; |
| Pfam | PF01077; NIR_SIR; 1; |
| Pfam | PF03460; NIR_SIR_ferr; 2; |
| PRINTS | PR00397; SIROHAEM; 1; |
| NCBIfam | TIGR02041; CysI; 1; |
Features
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| From: CYSI_ECOLI (P17846) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 434 | 434 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 440 | 440 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 479 | 479 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
| BINDING | 483 | 483 | /ligand="siroheme" /ligand_id="ChEBI:CHEBI:60052" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
C | ||||||||
| BINDING | 483 | 483 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
Additional information
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| Size range | 555-616 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |