HAMAP rule MF_01540
General rule information
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Accession | MF_01540 |
Dates | 22-AUG-2005 (Created)
1-JUN-2023 (Last updated, Version 16) |
Name | CysI |
Scope(s) |
Bacteria |
Template(s) | P17846 (CYSI_ECOLI); P17845 (CYSI_SALTY); O32213 (CYSI_BACSU); Q8KQT8 (CYSI_XANOR); [ Recover all ] |
Triggered by |
HAMAP; MF_01540 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | CYSI |
Protein name | RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component; Short=SiR-HP; Short=SiRHP; EC=1.8.1.2; |
Gene name | Name=cysI; |
Comments
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FUNCTION | Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. |
CATALYTIC ACTIVITY | Reaction=3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite; Xref=Rhea:RHEA:13801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359, ChEBI:CHEBI:29919, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.2; |
COFACTOR | Name=siroheme; Xref=ChEBI:CHEBI:60052; Note=Binds 1 siroheme per subunit.; |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.; |
PATHWAY | Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. |
SUBUNIT | Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein. |
SIMILARITY | Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. |
Keywords
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4Fe-4S |
Amino-acid biosynthesis |
Cysteine biosynthesis |
Heme |
Iron |
Iron-sulfur |
Metal-binding |
NADP |
Oxidoreductase |
Gene Ontology
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GO:0004783; Molecular function:sulfite reductase (NADPH) activity |
GO:0000103; Biological process:sulfate assimilation |
GO:0070814; Biological process:hydrogen sulfide biosynthetic process |
Cross-references
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PROSITE | PS00365; NIR_SIR; 1; |
Pfam | PF01077; NIR_SIR; 1; |
Pfam | PF03460; NIR_SIR_ferr; 2; |
PRINTS | PR00397; SIROHAEM; 1; |
NCBIfam | TIGR02041; CysI; 1; |
Features
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From: CYSI_ECOLI (P17846) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 434 | 434 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
BINDING | 440 | 440 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
BINDING | 479 | 479 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | ||||||||
BINDING | 483 | 483 | /ligand="siroheme" /ligand_id="ChEBI:CHEBI:60052" /ligand_part="Fe" /ligand_part_id="ChEBI:CHEBI:18248" /note="axial binding residue" |
C | ||||||||
BINDING | 483 | 483 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C |
Additional information
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Size range | 555-616 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |