Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
HAMAP rule MF_01547
General rule information
[?]
| Accession | MF_01547 |
| Dates | 22-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 28) |
| Name | RNA_methyltr_E |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0C0R7 (RLME_ECOLI); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_01547 (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | RLME |
| Protein name | RecName: Full=Ribosomal RNA large subunit methyltransferase E; EC=2.1.1.166; AltName: Full=23S rRNA Um2552 methyltransferase; AltName: Full=rRNA (uridine-2'-O-)-methyltransferase; |
| case <OC:Bacteria> | |
| Gene name | Name=rlmE; Synonyms=ftsJ, rrmJ; |
| else case <OC:Archaea> | |
| Gene name | Name=rlmE; |
| end case | |
Comments
[?]
| FUNCTION | Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit. |
| CATALYTIC ACTIVITY | Reaction=S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O- methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42720, Rhea:RHEA-COMP:10202, Rhea:RHEA-COMP:10203, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.166; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0008650; Molecular function:rRNA (uridine-2'-O-)-methyltransferase activity |
| GO:0006364; Biological process:rRNA processing |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
[?]
| PROSITE | PS50926; TRAM; 0-1; |
| Pfam | PF01728; FtsJ; 1; |
| Pfam | PF01938; TRAM; 0-1; |
| PIRSF | PIRSF005461; 23S_rRNA_mtase; 1; |
| NCBIfam | TIGR00438; RrmJ; 1; |
Features
[?]
| From: RLME_ECOLI (P0C0R7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 164 | 164 | /note="Proton acceptor" | K | ||||||||
| BINDING | 63 | 63 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | 1 | |||||||
| BINDING | 65 | 65 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[WF] | 1 | |||||||
| BINDING | 83 | 83 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | 1 | |||||||
| BINDING | 99 | 99 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DN] | 1 | |||||||
| BINDING | 124 | 124 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | 1 | |||||||
Additional information
[?]
| Size range | 189-279 amino acids |
| Related rules |
MF_03162 MF_03163 |
| Fusion | Nter: None Cter: None |