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HAMAP rule MF_01547

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General rule information [?]

Accession MF_01547
Dates 22-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 28)
Name RNA_methyltr_E
Scope(s) Bacteria
Template(s) P0C0R7 (RLME_ECOLI); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_01547 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RLME
Protein name RecName: Full=Ribosomal RNA large subunit methyltransferase E;
AltName: Full=23S rRNA Um2552 methyltransferase;
AltName: Full=rRNA (uridine-2'-O-)-methyltransferase;
case <OC:Bacteria>
Gene name Name=rlmE; Synonyms=ftsJ, rrmJ;
else case <OC:Archaea>
Gene name Name=rlmE;
end case

Comments [?]

FUNCTIONSpecifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.
CATALYTIC ACTIVITY Reaction=S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O- methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42720, Rhea:RHEA-COMP:10202, Rhea:RHEA-COMP:10203, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=;
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.

Keywords [?]

Gene Ontology [?]

GO:0008650; Molecular function:rRNA (uridine-2'-O-)-methyltransferase activity
GO:0006364; Biological process:rRNA processing
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS50926; TRAM; 0-1;
Pfam PF01728; FtsJ; 1;
Pfam PF01938; TRAM; 0-1;
PIRSF PIRSF005461; 23S_rRNA_mtase; 1;
NCBIfam TIGR00438; RrmJ; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 164 164 /note="Proton acceptor" K
BINDING 63 63 /ligand="S-adenosyl-L-methionine"
G 1
BINDING 65 65 /ligand="S-adenosyl-L-methionine"
[WF] 1
BINDING 83 83 /ligand="S-adenosyl-L-methionine"
D 1
BINDING 99 99 /ligand="S-adenosyl-L-methionine"
[DN] 1
BINDING 124 124 /ligand="S-adenosyl-L-methionine"
D 1

Additional information [?]

Size range 189-279 amino acids
Related rules MF_03162
Fusion Nter: None Cter: None

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