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HAMAP rule MF_01552

General rule information [?]

Accession	MF_01552
Dates	30-NOV-2005 (Created) 1-JUN-2023 (Last updated, Version 25)
Name	RimK
Scope(s)	Bacteria Cyanobacteriota Planctomycetota Pseudomonadota Archaea Methanomicrobia
Template(s)	P0C0U4 (RIMK_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_01552 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RIMK
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Protein name	RecName: Full=Ribosomal protein bS6--L-glutamate ligase; EC=6.3.2.-; AltName: Full=Poly-alpha-glutamate synthase; AltName: Full=Ribosomal protein bS6 modification protein;
else
Protein name	RecName: Full=Probable alpha-L-glutamate ligase; EC=6.3.2.-;
end case
Gene name	Name=rimK;

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
FUNCTION	An L-glutamate ligase that catalyzes the ATP-dependent post- translational addition of glutamate residues to the C-terminus of ribosomal protein bS6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.
end case
case <FTGroup:1>
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 magnesium or manganese ions per subunit.;
end case
SIMILARITY	Belongs to the RimK family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
case <OCellular component:Escherichia> or <OC:Shigella> or <OC:Salmonella>
GO:0018169; Molecular function:ribosomal S6-glutamic acid ligase activity
GO:0018410; Biological process:C-terminal protein amino acid modification
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0016881; Molecular function:acid-amino acid ligase activity
end case

Cross-references [?]

Features [?]

From: RIMK_ECOLI (P0C0U4)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	178	179	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		[EDQ]-[YF]
BINDING	211	213	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		R-[SA]-N
BINDING	248	248	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1"		D	1
BINDING	248	248	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1"		D	1
BINDING	260	260	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1"		E	1
BINDING	260	260	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2"		E	1
BINDING	260	260	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1"		E	1
BINDING	260	260	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2"		E	1
BINDING	262	262	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2"		N	1
BINDING	262	262	/ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2"		N	1
BINDING	141	141	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		K
BINDING	187	187	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		D

Additional information [?]

Size range	291-313 amino acids
Related rules	None
Fusion	Nter: <Unknown> Cter: None
Comments	The ribosomal protein bS6-modifying function does not apply to all RimK family members since some bacteria do not possess a rpsF gene in their genome, or the C-terminal sequence of RpsF is not conserved, or does not end with a glutamate residue.