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Annotation rule MF_01552
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General rule information [?]

Accession MF_01552
Dates 30-NOV-2005 (Created)
19-NOV-2019 (Last updated, Version 17)
Name RimK
Bacteria; Cyanobacteria
Bacteria; Planctomycetes
Bacteria; Proteobacteria
Archaea; Methanomicrobia
Template P0C0U4 (RIMK_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Protein name
RecName: Full=Ribosomal protein S6--L-glutamate ligase;
EC 6.3.2.-;
AltName: Full=Poly-alpha-glutamate synthase;
AltName: Full=Ribosomal protein S6 modification protein;
Protein name
RecName: Full=Probable alpha-L-glutamate ligase;
EC 6.3.2.-;
end case
Gene name

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Function Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.
end case
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 2 magnesium or manganese ions per subunit.
end case
Similarity Belongs to the RimK family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
GO:0018169; Molecular function: ribosomal S6-glutamic acid ligase activity.
GO:0018410; Biological process: C-terminal protein amino acid modification.
GO:0016881; Molecular function: acid-amino acid ligase activity.
end case

Cross-references [?]

PROSITE PS50975; ATP_GRASP; 1; trigger=PRU00409;
Pfam PF02222; ATP-grasp; 1;
TIGRFAMs TIGR00768; RimK_fam; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     178     179       ATP     [EDQ]-[YF]  
NP_BIND     211     213       ATP     R-[SA]-N  
METAL     248     248       Magnesium or manganese 1     D   1
METAL     260     260       Magnesium or manganese 1     E   1
METAL     260     260       Magnesium or manganese 2     E   1
METAL     262     262       Magnesium or manganese 2     N   1
BINDING     141     141       ATP     K  
BINDING     187     187       ATP     D  

Additional information [?]

Size range 291-313 amino acids
Related rules None
Fusion Nter: <Unknown>; Cter: None
Comments The ribosomal protein S6-modifying function does not apply to all RimK family members since some bacteria do not possess a rpsF gene in their genome or the C-terminal sequence of RpsF is not conserved or does not end with a glutamate residue.