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HAMAP rule MF_01609

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General rule information [?]

Accession MF_01609
Dates 12-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 26)
Name Glu_cys_ligase_2
Scope(s) Bacteria
Archaea
Template(s) D4GZN2 (GCS2_HALVD); P77213 (GCS2_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01609 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GCS2
case <OC:Archaea>
Protein name RecName: Full=Glutamate--cysteine ligase;
                 EC=6.3.2.2;
AltName: Full=Gamma-glutamylcysteine synthetase;
                 Short=Gamma-GCS;
                 Short=GCS;
Gene name Name=gshA;
else
Protein name RecName: Full=Putative glutamate--cysteine ligase 2;
                 EC=6.3.2.2;
AltName: Full=Gamma-glutamylcysteine synthetase 2;
                 Short=Gamma-GCS 2;
                 Short=GCS 2;
end case

Comments [?]

case <OC:Archaea>
FUNCTIONCatalyzes the synthesis of gamma-glutamylcysteine (gamma-GC), the main low-molecular-weight thiol compound instead of glutathione in halophilic archaea.
else
FUNCTIONATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.
end case
CATALYTIC ACTIVITY Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L- cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
case <OC:Enterobacterales>
SUBUNITHomodimer.
end case
SIMILARITYBelongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.

Keywords [?]


Gene Ontology [?]

case <OCellular component:Archaea>
GO:0004357; Molecular function:glutamate-cysteine ligase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0016879; Molecular function:ligase activity, forming carbon-nitrogen bonds
end case

Cross-references [?]

Pfam PF04107; GCS2; 1;
NCBIfam TIGR02050; GshA_cyan_rel; 1;

Features [?]


Additional information [?]

Size range 320-426 amino acids
Related rules None
Fusion Nter: None Cter: <Unknown>; <Peptidase M20>



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