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HAMAP rule MF_01612

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General rule information [?]

Accession MF_01612
Dates 11-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 26)
Name FO_synth_sub2
Bacteria; Cyanobacteriota
Archaea; Euryarchaeota
Template Q58826 (COFH_METJA)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase;
AltName: Full=FO synthase subunit 2;
Gene name

Comments [?]

Function Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.
Catalytic activity RHEA:55200: 5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
end case
Pathway Cofactor biosynthesis; coenzyme F0 biosynthesis.
Subunit The FO synthase complex consists of two subunits, CofG and CofH.
Similarity Belongs to the radical SAM superfamily. CofH family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0005506; Molecular function: iron ion binding.
end case
GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
PIRSF PIRSF004762; CHP00423; 1;
NCBIfam TIGR00423; TIGR00423; 1;

Features [?]

From: COFH_METJA (Q58826)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     59     59       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1
BINDING     63     63       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1
BINDING     66     66       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet     C   1

Additional information [?]

Size range 338-406 amino acids
Related rules None
Fusion Nter: MF_01611 (cofG); Cter: None
Comments Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts.