HAMAP rule MF_01612

General rule information [?]

Accession	MF_01612
Dates	11-APR-2005 (Created) 1-JUN-2023 (Last updated, Version 26)

Name	FO_synth_sub2

Scope

Bacteria; Cyanobacteriota

Archaea; Euryarchaeota

Template

Q58826 (COFH_METJA)

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

COFH

Protein name

RecName:		Full=5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase;
		EC 2.5.1.147;
AltName:		Full=FO synthase subunit 2;

Gene name

cofH

Comments [?]

Function	Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.
Catalytic activity	RHEA:55200: 5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine EC 2.5.1.147

case <FTGroup:1>

Cofactor

[4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

end case

Pathway	Cofactor biosynthesis; coenzyme F0 biosynthesis.
Subunit	The FO synthase complex consists of two subunits, CofG and CofH.
Similarity	Belongs to the radical SAM superfamily. CofH family.

Keywords [?]

case <FTGroup:1>

end case

Gene Ontology [?]

case <FTGroup:1>

GO:0005506; Molecular function: iron ion binding.

end case

GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.

Cross-references [?]

Features [?]

From: COFH_METJA (Q58826)

Key From To Description Tag Condition FTGroup

BINDING 59 59 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 63 63 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

BINDING 66 66 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet C 1

Additional information [?]

Size range	338-406 amino acids
Related rules	None
Fusion	Nter: MF_01611 (cofG); Cter: None
Comments	Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts.