HAMAP logo

HAMAP rule MF_01612

Send feedback

General rule information [?]

Accession MF_01612
Dates 11-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 26)
Name FO_synth_sub2
Scope(s) Bacteria
Template(s) Q58826 (COFH_METJA); [ Recover all ]
Triggered by HAMAP; MF_01612 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier COFH
Protein name RecName: Full=5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase;
AltName: Full=FO synthase subunit 2;
Gene name Name=cofH;

Comments [?]

FUNCTIONCatalyzes the radical-mediated synthesis of 5-amino-5-(4- hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D- ribitylamino)uracil and L-tyrosine.
CATALYTIC ACTIVITY Reaction=5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L- methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4- hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L- methionine; Xref=Rhea:RHEA:55200, ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:17319, ChEBI:CHEBI:57844, ChEBI:CHEBI:58315, ChEBI:CHEBI:59789, ChEBI:CHEBI:77846, ChEBI:CHEBI:85936; EC=;
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
PATHWAYCofactor biosynthesis; coenzyme F0 biosynthesis.
SUBUNITThe FO synthase complex consists of two subunits, CofG and CofH.
SIMILARITYBelongs to the radical SAM superfamily. CofH family.

Keywords [?]

Gene Ontology [?]

case <FTGroup:1>
GO:0005506; Molecular function:iron ion binding
end case
GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
PIRSF PIRSF004762; CHP00423; 1;
NCBIfam TIGR00423; TIGR00423; 1;

Features [?]

From: COFH_METJA (Q58826)
Key From To Description Tag Condition FTGroup
BINDING 59 59 /ligand="[4Fe-4S] cluster"
C 1
BINDING 63 63 /ligand="[4Fe-4S] cluster"
C 1
BINDING 66 66 /ligand="[4Fe-4S] cluster"
C 1

Additional information [?]

Size range 338-406 amino acids
Related rules None
Fusion Nter: MF_01611 (cofG) Cter: None
Comments Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts.

View rule in raw text format (no links)