HAMAP rule MF_01615
General rule information
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| Accession | MF_01615 |
| Dates | 13-JUN-2005 (Created)
1-JUN-2023 (Last updated, Version 26) |
| Name | PdxT |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P37528 (PDXT_BACSU); Q9WYU3 (PDXT_THEMA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01615 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PDXT |
| Protein name | RecName: Full=Pyridoxal 5'-phosphate synthase subunit PdxT; EC=4.3.3.6; AltName: Full=Pyridoxal 5'-phosphate synthase glutaminase subunit; EC=3.5.1.2; AltName: Full=Pdx2; |
| Gene name | Name=pdxT; |
Comments
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| FUNCTION | Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS. |
| CATALYTIC ACTIVITY | Reaction=aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'- phosphate; Xref=Rhea:RHEA:31507, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:43474, ChEBI:CHEBI:58273, ChEBI:CHEBI:58359, ChEBI:CHEBI:59776, ChEBI:CHEBI:597326; EC=4.3.3.6; |
| CATALYTIC ACTIVITY | Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2; |
| PATHWAY | Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis. |
| SUBUNIT | In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer. |
| SIMILARITY | Belongs to the glutaminase PdxT/SNO family. |
Keywords
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Gene Ontology
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| GO:0004359; Molecular function:glutaminase activity |
| GO:0036381; Molecular function:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity |
| GO:0006543; Biological process:glutamine catabolic process |
| GO:0042823; Biological process:pyridoxal phosphate biosynthetic process |
Cross-references
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| Pfam | PF01174; SNO; 1; |
| PRINTS | PR00096; GATASE; 1; |
| PROSITE | PS01236; PDXT_SNO_1; 1; |
| PROSITE | PS51130; PDXT_SNO_2; 1; |
| NCBIfam | TIGR03800; PLP_synth_Pdx2; 1; |
Features
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| From: PDXT_BACSU (P37528) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 47 | 49 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
G-x-S | ||||||||
| BINDING | 134 | 135 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
I-R | ||||||||
| ACT_SITE | 79 | 79 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 170 | 170 | /note="Charge relay system" | H | ||||||||
| ACT_SITE | 172 | 172 | /note="Charge relay system" | E | ||||||||
| BINDING | 106 | 106 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
R | ||||||||
Additional information
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| Size range | 173-236 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |