HAMAP rule MF_01615
General rule information
[?]
| Accession | MF_01615 |
| Dates | 13-JUN-2005 (Created) 1-JUN-2023 (Last updated, Version 26) |
| Name | PdxT |
| Scope | Bacteria
Archaea |
| Templates | P37528 (PDXT_BACSU); Q9WYU3 (PDXT_THEMA): [Recover all] |
| Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier |
|
| Protein name |
|
||||||||||||||||||||
| Gene name |
|
Comments
[?]
| Function | Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS. |
| Catalytic activity | RHEA:31507: aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate
EC 4.3.3.6 |
| RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+)
EC 3.5.1.2 |
|
| Pathway | Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis. |
| Subunit | In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer. |
| Similarity | Belongs to the glutaminase PdxT/SNO family. |
Keywords
[?]
Gene Ontology
[?]
GO:0004359; Molecular function: glutaminase activity.
GO:0036381; Molecular function: pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity.
GO:0006543; Biological process: glutamine catabolic process.
GO:0042823; Biological process: pyridoxal phosphate biosynthetic process.
GO:0036381; Molecular function: pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity.
GO:0006543; Biological process: glutamine catabolic process.
GO:0042823; Biological process: pyridoxal phosphate biosynthetic process.
Cross-references
[?]
| Pfam | PF01174; SNO; 1; |
| PRINTS | PR00096; GATASE; 1; |
| PROSITE | PS01236; PDXT_SNO_1; 1; |
| PS51130; PDXT_SNO_2; 1; | |
| NCBIfam | TIGR03800; PLP_synth_Pdx2; 1; |
Features
[?]
| From: PDXT_BACSU (P37528) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 47 | 49 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359 | G-x-S | ||||||||
| BINDING | 134 | 135 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359 | I-R | ||||||||
| ACT_SITE | 79 | 79 | Nucleophile | C | ||||||||
| ACT_SITE | 170 | 170 | Charge relay system | H | ||||||||
| ACT_SITE | 172 | 172 | Charge relay system | E | ||||||||
| BINDING | 106 | 106 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359 | R | ||||||||
Additional information
[?]
| Size range | 173-236 amino acids |
| Related rules | None |
| Fusion | None |