HAMAP rule MF_01617

General rule information [?]

Accession	MF_01617
Dates	11-NOV-2005 (Created) 1-JUN-2023 (Last updated, Version 29)

Name

FadJ

Scope

Bacteria; Gammaproteobacteria

Template

P77399 (FADJ_ECOLI)

Triggered by

HAMAP; MF_01617 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

FADJ

Protein name

RecName:		Full=Fatty acid oxidation complex subunit alpha;
Includes:
	RecName:		Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase;
			EC 4.2.1.17;
			EC 5.1.2.3;
Includes:
	RecName:		Full=3-hydroxyacyl-CoA dehydrogenase;
			EC 1.1.1.35;

Gene name

fadJ

Comments [?]

Function	Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.
Catalytic activity	RHEA:16105: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O EC 4.2.1.17
	RHEA:20724: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O EC 4.2.1.17
	RHEA:22432: a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH EC 1.1.1.35
	RHEA:21760: (3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA EC 5.1.2.3
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit	Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).
Subcellular location	Cytoplasm.
Similarity	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
	In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Keywords [?]

Cytoplasm
Fatty acid metabolism
Isomerase
Lipid degradation
Lipid metabolism
Lyase
Multifunctional enzyme
NAD
Oxidoreductase

Gene Ontology [?]

GO:0003857; Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity.
GO:0004300; Molecular function: enoyl-CoA hydratase activity.
GO:0008692; Molecular function: 3-hydroxybutyryl-CoA epimerase activity.
GO:0006631; Biological process: fatty acid metabolic process.
GO:0016042; Biological process: lipid catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS00067; 3HCDH; 1;
	PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam	PF00725; 3HCDH; 2;
	PF02737; 3HCDH_N; 1;
	PF00378; ECH; 1;
NCBIfam	TIGR02440; FadJ; 1;

Features [?]

From: FADJ_ECOLI (P77399)

Key From To Description Tag Condition FTGroup

REGION Nter 190 Enoyl-CoA hydratase

REGION 306 Cter 3-hydroxyacyl-CoA dehydrogenase

SITE 118 118 Important for catalytic activity E

SITE 140 140 Important for catalytic activity E

Additional information [?]

Size range	699-780 amino acids
Related rules	None
Fusion	None

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