HAMAP rule MF_01617
General rule information
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| Accession | MF_01617 |
| Dates | 11-NOV-2005 (Created)
7-MAY-2024 (Last updated, Version 30) |
| Name | FadJ |
| Scope(s) |
Bacteria Gammaproteobacteria |
| Template(s) | P77399 (FADJ_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01617 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | FADJ |
| Protein name | RecName: Full=Fatty acid oxidation complex subunit alpha; Includes: RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; EC=4.2.1.17; EC=5.1.2.3; Includes: RecName: Full=3-hydroxyacyl-CoA dehydrogenase; EC=1.1.1.35; |
| Gene name | Name=fadJ; |
Comments
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| FUNCTION | Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3- hydroxyacyl-CoA dehydrogenase activities. |
| CATALYTIC ACTIVITY | Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, ChEBI:CHEBI:58856; EC=4.2.1.17; |
| CATALYTIC ACTIVITY | Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521, ChEBI:CHEBI:137480; EC=4.2.1.17; |
| CATALYTIC ACTIVITY | Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35; |
| CATALYTIC ACTIVITY | Reaction=(3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA; Xref=Rhea:RHEA:21760, ChEBI:CHEBI:57315, ChEBI:CHEBI:57316; EC=5.1.2.3; |
| PATHWAY | Lipid metabolism; fatty acid beta-oxidation. |
| SUBUNIT | Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI). |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. |
| SIMILARITY | In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. |
Keywords
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| Cytoplasm |
| Fatty acid metabolism |
| Isomerase |
| Lipid degradation |
| Lipid metabolism |
| Lyase |
| Multifunctional enzyme |
| NAD |
| Oxidoreductase |
Gene Ontology
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| GO:0003857; Molecular function:3-hydroxyacyl-CoA dehydrogenase activity |
| GO:0004300; Molecular function:enoyl-CoA hydratase activity |
| GO:0008692; Molecular function:3-hydroxybutyryl-CoA epimerase activity |
| GO:0006635; Biological process:fatty acid beta-oxidation |
| GO:0016042; Biological process:lipid catabolic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00067; 3HCDH; 1; |
| PROSITE | PS00166; ENOYL_COA_HYDRATASE; 1; |
| Pfam | PF00725; 3HCDH; 2; |
| Pfam | PF02737; 3HCDH_N; 1; |
| Pfam | PF00378; ECH; 1; |
| NCBIfam | TIGR02440; FadJ; 1; |
Features
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| From: FADJ_ECOLI (P77399) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 190 | /note="Enoyl-CoA hydratase" | |||||||||
| REGION | 306 | Cter | /note="3-hydroxyacyl-CoA dehydrogenase" | |||||||||
| SITE | 118 | 118 | /note="Important for catalytic activity" | E | ||||||||
| SITE | 140 | 140 | /note="Important for catalytic activity" | E | ||||||||
Additional information
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| Size range | 699-780 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |