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HAMAP rule MF_01617

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General rule information [?]

Accession MF_01617
Dates 11-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 29)
Name FadJ
Scope(s) Bacteria
Template(s) P77399 (FADJ_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01617 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FADJ
Protein name RecName: Full=Fatty acid oxidation complex subunit alpha;
RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase;
RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
Gene name Name=fadJ;

Comments [?]

FUNCTIONCatalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3- hydroxyacyl-CoA dehydrogenase activities.
CATALYTIC ACTIVITY Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, ChEBI:CHEBI:58856; EC=;
CATALYTIC ACTIVITY Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521, ChEBI:CHEBI:137480; EC=;
CATALYTIC ACTIVITY Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=;
CATALYTIC ACTIVITY Reaction=(3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA; Xref=Rhea:RHEA:21760, ChEBI:CHEBI:57315, ChEBI:CHEBI:57316; EC=;
PATHWAYLipid metabolism; fatty acid beta-oxidation.
SUBUNITHeterotetramer of two alpha chains (FadJ) and two beta chains (FadI).
SIMILARITYIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
SIMILARITYIn the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Keywords [?]

Gene Ontology [?]

GO:0003857; Molecular function:3-hydroxyacyl-CoA dehydrogenase activity
GO:0004300; Molecular function:enoyl-CoA hydratase activity
GO:0008692; Molecular function:3-hydroxybutyryl-CoA epimerase activity
GO:0006631; Biological process:fatty acid metabolic process
GO:0016042; Biological process:lipid catabolic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS00067; 3HCDH; 1;
Pfam PF00725; 3HCDH; 2;
Pfam PF02737; 3HCDH_N; 1;
Pfam PF00378; ECH; 1;
NCBIfam TIGR02440; FadJ; 1;

Features [?]

From: FADJ_ECOLI (P77399)
Key From To Description Tag Condition FTGroup
REGION Nter 190 /note="Enoyl-CoA hydratase"
REGION 306 Cter /note="3-hydroxyacyl-CoA dehydrogenase"
SITE 118 118 /note="Important for catalytic activity" E
SITE 140 140 /note="Important for catalytic activity" E

Additional information [?]

Size range 699-780 amino acids
Related rules None
Fusion Nter: None Cter: None

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