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Annotation rule MF_01617
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General rule information [?]

Accession MF_01617
Dates 11-NOV-2005 (Created)
19-NOV-2019 (Last updated, Version 25)
Name FadJ
Scope
Bacteria; Gammaproteobacteria
Template P77399 (FADJ_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
FADJ
Protein name
RecName: Full=Fatty acid oxidation complex subunit alpha;
Includes:
RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase;
EC 4.2.1.17;
EC 5.1.2.3;
Includes:
RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
EC 1.1.1.35;
Gene name
fadJ

Comments [?]

Function Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.
Catalytic activity RHEA:16105: a (3S)-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O
EC 4.2.1.17
RHEA:20724: a 4-saturated-(3S)-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O
EC 4.2.1.17
RHEA:22432: a (3S)-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH
EC 1.1.1.35
RHEA:21760: (3S)-hydroxybutanoyl-CoA = (3R)-hydroxybutanoyl-CoA
EC 5.1.2.3
Pathway Lipid metabolism; fatty acid beta-oxidation.
Subunit Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Keywords [?]


Gene Ontology [?]

GO:0003857; Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity.
GO:0004300; Molecular function: enoyl-CoA hydratase activity.
GO:0008692; Molecular function: 3-hydroxybutyryl-CoA epimerase activity.
GO:0006631; Biological process: fatty acid metabolic process.
GO:0016042; Biological process: lipid catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00067; 3HCDH; 1;
PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam PF00725; 3HCDH; 2;
PF02737; 3HCDH_N; 1;
PF00378; ECH; 1;
TIGRFAMs TIGR02440; FadJ; 1;

Features [?]

From: FADJ_ECOLI (P77399)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     190       Enoyl-CoA hydratase        
REGION     306     Cter       3-hydroxyacyl-CoA dehydrogenase        
SITE     118     118       Important for catalytic activity     E  
SITE     140     140       Important for catalytic activity     E  

Additional information [?]

Size range 699-780 amino acids
Related rules None
Fusion None