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HAMAP rule MF_01630

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General rule information [?]

Accession MF_01630
Dates 18-JAN-2006 (Created)
1-JUN-2023 (Last updated, Version 38)
Name Nitrate_reduct_NapA
Scope(s) Bacteria
Template(s) P33937 (NAPA_ECOLI); P81186 (NAPA_DESDA); P39185 (NAPA_CUPNH); Q53176 (NAPA_CERS4); [ Recover all ]
Triggered by HAMAP; MF_01630 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NAPA
case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)
Protein name RecName: Full=Periplasmic nitrate reductase;
                 EC=1.9.6.1;
                 Flags: Precursor;
else
Protein name RecName: Full=Nitrate reductase;
                 EC=1.9.6.1;
                 Flags: Precursor;
end case
Gene name Name=napA;

Comments [?]

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)
FUNCTIONCatalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
else
FUNCTIONCatalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
end case
CATALYTIC ACTIVITY Reaction=2 Fe(II)-[cytochrome] + 2 H(+) + nitrate = 2 Fe(III)- [cytochrome] + H2O + nitrite; Xref=Rhea:RHEA:12909, Rhea:RHEA- COMP:11777, Rhea:RHEA-COMP:11778, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.9.6.1;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
COFACTOR Name=Mo-bis(molybdopterin guanine dinucleotide); Xref=ChEBI:CHEBI:60539; Note=Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo- bis-MGD) cofactor per subunit.;
case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)
SUBUNITComponent of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.
SUBCELLULAR LOCATIONPeriplasm.
else
SUBUNITComponent of the nitrate reductase NapAB complex composed of NapA and NapB.
SUBCELLULAR LOCATIONSecreted. Note=Membrane-associated.
end case
case <Feature:PS51318>
PTMPredicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
end case
SIMILARITYBelongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Keywords [?]

4Fe-4S
Electron transport
Iron
Iron-sulfur
Metal-binding
Molybdenum
Nitrate assimilation
Oxidoreductase
case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)
Periplasm
end case
Signal
Transport

Gene Ontology [?]

GO:0009055; Molecular function:electron transfer activity
GO:0005506; Molecular function:iron ion binding
GO:0008940; Molecular function:nitrate reductase activity
GO:0006777; Biological process:Mo-molybdopterin cofactor biosynthetic process
GO:0042128; Biological process:nitrate assimilation
case <OCellular component:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)
GO:0042597; Cellular component:periplasmic space
end case

Cross-references [?]

PROSITE PS51669; 4FE4S_MOW_BIS_MGD; 1;
PROSITE PS00551; MOLYBDOPTERIN_PROK_1; 1;
PROSITE PS00490; MOLYBDOPTERIN_PROK_2; 1;
PROSITE PS00932; MOLYBDOPTERIN_PROK_3; 1;
PROSITE PS51318; TAT; 0-1;
Pfam PF04879; Molybdop_Fe4S4; 1;
Pfam PF00384; Molybdopterin; 1;
Pfam PF01568; Molydop_binding; 1;
Pfam PF10518; TAT_signal; 0-1;
NCBIfam TIGR01706; NAPA; 1;
NCBIfam TIGR01409; TAT_signal_seq; 0-1;
General Signal; -; 1;

Features [?]

From: NAPA_ECOLI (P33937)
Key From To Description Tag Condition FTGroup
BINDING 212 219 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 W-G-[AS]-N-M-A-E-M
BINDING 243 247 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 S-T-[YF]-x-H
BINDING 262 264 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 [QG]-[ST]-D
BINDING 508 509 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 S-[DE]
BINDING 718 727 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 [ST]-[GM]-R-[VI]-[ILV]-[ED]-H-W-H-[TS]
BINDING 46 46 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C
BINDING 49 49 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C
BINDING 53 53 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C
BINDING 81 81 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"		 C
BINDING 83 83 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 K
BINDING 150 150 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 Q
BINDING 175 175 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 N
BINDING 179 179 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 C
BINDING 372 372 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 M
BINDING 376 376 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 Q
BINDING 482 482 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 N
BINDING 531 531 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 K
BINDING 558 558 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 D
BINDING 794 794 /ligand="substrate" [FW]
BINDING 802 802 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 N
BINDING 819 819 /ligand="Mo-bis(molybdopterin guanine dinucleotide)"
/ligand_id="ChEBI:CHEBI:60539"		 K

Additional information [?]

Size range 754-950 amino acids
Related rules None
Fusion Nter: None Cter: None



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