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Annotation rule MF_01630
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General rule information [?]

Accession MF_01630
Dates 18-JAN-2006 (Created)
14-MAY-2022 (Last updated, Version 35)
Name Nitrate_reduct_NapA
Scope
Bacteria
Templates P33937 (NAPA_ECOLI); P81186 (NAPA_DESDA); P39185 (NAPA_CUPNH); Q53176 (NAPA_CERS4): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NAPA
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Protein name
RecName: Full=Periplasmic nitrate reductase;
EC 1.9.6.1;
Flags: Precursor;
else
Protein name
RecName: Full=Nitrate reductase;
EC 1.9.6.1;
Flags: Precursor;
end case
Gene name
napA

Comments [?]

case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Function Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
else
Function Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
end case
Catalytic activity RHEA:12909: 2 Fe(II)-[cytochrome] + 2 H(+) + nitrate = 2 Fe(III)-[cytochrome] + H2O + nitrite
EC 1.9.6.1
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
Mo-bis(molybdopterin guanine dinucleotide)
Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Subunit Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location Periplasm.
else
Subunit Component of the nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location Secreted. Note=Membrane-associated.
end case
case <Feature:PS51318>
Ptm Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
end case
Similarity Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Keywords [?]

4Fe-4S
Electron transport
Iron
Iron-sulfur
Metal-binding
Molybdenum
Nitrate assimilation
Oxidoreductase
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Periplasm
end case
Signal
Transport

Gene Ontology [?]

GO:0009055; Molecular function: electron transfer activity.
GO:0005506; Molecular function: iron ion binding.
GO:0008940; Molecular function: nitrate reductase activity.
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
GO:0042128; Biological process: nitrate assimilation.
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
GO:0042597; Cellular component: periplasmic space.
end case

Cross-references [?]

PROSITE PS51669; 4FE4S_MOW_BIS_MGD; 1; trigger=PRU01004;
PS00551; MOLYBDOPTERIN_PROK_1; 1;
PS00490; MOLYBDOPTERIN_PROK_2; 1;
PS00932; MOLYBDOPTERIN_PROK_3; 1;
PS51318; TAT; 0-1; trigger=PRU00648;
Pfam PF04879; Molybdop_Fe4S4; 1;
PF00384; Molybdopterin; 1;
PF01568; Molydop_binding; 1;
PF10518; TAT_signal; 0-1;
TIGRFAMs TIGR01706; NAPA; 1;
TIGR01409; TAT_signal_seq; 0-1;

Computed features [?]

case not <Feature:PS51318>
General Signal; -; 1; trigger=yes;
end case

Features [?]

From: NAPA_ECOLI (P33937)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     212     219       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     W-G-[AS]-N-M-A-E-M  
BINDING (Optional)     243     247       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     S-T-[YF]-x-H  
BINDING (Optional)     262     264       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     [QG]-[ST]-D  
BINDING (Optional)     508     509       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     S-[DE]  
BINDING (Optional)     718     727       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     [ST]-[GM]-R-[VI]-[ILV]-[ED]-H-W-H-[TS]  
BINDING     46     46       /ligand="[4Fe-4S] cluster" /ligand_id="49883     C  
BINDING     49     49       /ligand="[4Fe-4S] cluster" /ligand_id="49883     C  
BINDING     53     53       /ligand="[4Fe-4S] cluster" /ligand_id="49883     C  
BINDING     81     81       /ligand="[4Fe-4S] cluster" /ligand_id="49883     C  
BINDING (Optional)     83     83       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     K  
BINDING (Optional)     150     150       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     Q  
BINDING (Optional)     175     175       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     N  
BINDING (Optional)     179     179       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     C  
BINDING (Optional)     372     372       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     M  
BINDING (Optional)     376     376       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     Q  
BINDING (Optional)     482     482       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     N  
BINDING (Optional)     531     531       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     K  
BINDING (Optional)     558     558       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     D  
BINDING (Optional)     794     794       /ligand="substrate     [FW]  
BINDING (Optional)     802     802       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     N  
BINDING (Optional)     819     819       /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="60539     K  

Additional information [?]

Size range 754-950 amino acids
Related rules None
Fusion None


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