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Annotation rule MF_01630
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General rule information [?]

Accession MF_01630
Dates 18-JAN-2006 (Created)
19-NOV-2019 (Last updated, Version 30)
Name Nitrate_reduct_NapA
Scope
Bacteria
Templates P33937 (NAPA_ECOLI); P81186 (NAPA_DESDA); P39185 (NAPA_CUPNH); Q53176 (NAPA_RHOS4): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NAPA
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Protein name
RecName: Full=Periplasmic nitrate reductase;
EC 1.9.6.1;
Flags: Precursor;
else
Protein name
RecName: Full=Nitrate reductase;
EC 1.9.6.1;
Flags: Precursor;
end case
Gene name
napA

Comments [?]

case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Function Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
else
Function Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.
end case
Catalytic activity RHEA:12909: 2 [Fe(II)cytochrome] + 2 H(+) + nitrate = 2 [Fe(III)cytochrome] + H2O + nitrite
EC 1.9.6.1
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
Mo-bis(molybdopterin guanine dinucleotide)
Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
Subunit Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location Periplasm.
else
Subunit Component of the nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location Secreted. Note=Membrane-associated.
end case
case <Feature:PS51318>
Ptm Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
end case
Similarity Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Keywords [?]

case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
end case

Gene Ontology [?]

GO:0009055; Molecular function: electron transfer activity.
GO:0005506; Molecular function: iron ion binding.
GO:0008940; Molecular function: nitrate reductase activity.
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
GO:0042128; Biological process: nitrate assimilation.
GO:0055114; Biological process: oxidation-reduction process.
case <OC:Proteobacteria> or (defined <Property:Membrane> and <Property:Membrane=2>)
GO:0042597; Cellular component: periplasmic space.
end case

Cross-references [?]

PROSITE PS51669; 4FE4S_MOW_BIS_MGD; 1; trigger=PRU01004;
PS00551; MOLYBDOPTERIN_PROK_1; 1;
PS00490; MOLYBDOPTERIN_PROK_2; 1;
PS00932; MOLYBDOPTERIN_PROK_3; 1;
PS51318; TAT; 0-1; trigger=PRU00648;
Pfam PF04879; Molybdop_Fe4S4; 1;
PF00384; Molybdopterin; 1;
PF01568; Molydop_binding; 1;
PF10518; TAT_signal; 0-1;
TIGRFAMs TIGR01706; NAPA; 1;
TIGR01409; TAT_signal_seq; 0-1;

Computed features [?]

case not <Feature:PS51318>
General Signal; -; 1; trigger=yes;
end case

Features [?]

From: NAPA_ECOLI (P33937)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     212     219       Mo-bis(molybdopterin guanine dinucleotide) binding     W-G-[AS]-N-M-A-E-M  
REGION (Optional)     243     247       Mo-bis(molybdopterin guanine dinucleotide) binding     S-T-[YF]-x-H  
REGION (Optional)     262     264       Mo-bis(molybdopterin guanine dinucleotide) binding     [QG]-[ST]-D  
REGION (Optional)     508     509       Mo-bis(molybdopterin guanine dinucleotide) binding     S-[DE]  
REGION (Optional)     718     727       Mo-bis(molybdopterin guanine dinucleotide) binding     [ST]-[GM]-R-[VI]-[ILV]-[ED]-H-W-H-[TS]  
METAL     46     46       Iron-sulfur (4Fe-4S)     C  
METAL     49     49       Iron-sulfur (4Fe-4S)     C  
METAL     53     53       Iron-sulfur (4Fe-4S)     C  
METAL     81     81       Iron-sulfur (4Fe-4S)     C  
BINDING (Optional)     83     83       Mo-bis(molybdopterin guanine dinucleotide)     K  
BINDING (Optional)     150     150       Mo-bis(molybdopterin guanine dinucleotide)     Q  
BINDING (Optional)     175     175       Mo-bis(molybdopterin guanine dinucleotide)     N  
BINDING (Optional)     179     179       Mo-bis(molybdopterin guanine dinucleotide)     C  
BINDING (Optional)     372     372       Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogen     M  
BINDING (Optional)     376     376       Mo-bis(molybdopterin guanine dinucleotide)     Q  
BINDING (Optional)     482     482       Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogen     N  
BINDING (Optional)     531     531       Mo-bis(molybdopterin guanine dinucleotide)     K  
BINDING (Optional)     558     558       Mo-bis(molybdopterin guanine dinucleotide)     D  
BINDING (Optional)     794     794       Substrate; via amide nitrogen     [FW]  
BINDING (Optional)     802     802       Mo-bis(molybdopterin guanine dinucleotide)     N  
BINDING (Optional)     819     819       Mo-bis(molybdopterin guanine dinucleotide)     K  

Additional information [?]

Size range 754-950 amino acids
Related rules None
Fusion None