HAMAP rule MF_01630

General rule information [?]

Accession	MF_01630
Dates	18-JAN-2006 (Created) 26-JAN-2023 (Last updated, Version 37)

Name	Nitrate_reduct_NapA

Scope

Bacteria

Templates

P33937 (NAPA_ECOLI); P81186 (NAPA_DESDA); P39185 (NAPA_CUPNH); Q53176 (NAPA_CERS4): [Recover all]

Triggered by

HAMAP; MF_01630 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

NAPA

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)

Protein name

RecName:		Full=Periplasmic nitrate reductase;
		EC 1.9.6.1;
Flags:		Precursor;

else

Protein name

RecName:		Full=Nitrate reductase;
		EC 1.9.6.1;
Flags:		Precursor;

end case

Gene name

napA

Comments [?]

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)

Function

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.

else

Function

Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.

end case

Catalytic activity	RHEA:12909: 2 Fe(II)-[cytochrome] + 2 H(+) + nitrate = 2 Fe(III)-[cytochrome] + H2O + nitrite EC 1.9.6.1
Cofactor	[4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster.
	Mo-bis(molybdopterin guanine dinucleotide) Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)

Subunit	Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location	Periplasm.

else

Subunit	Component of the nitrate reductase NapAB complex composed of NapA and NapB.
Subcellular location	Secreted. Note=Membrane-associated.

end case

case <Feature:PS51318>

Ptm	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

end case

Similarity

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Keywords [?]

4Fe-4S
Electron transport
Iron
Iron-sulfur
Metal-binding
Molybdenum
Nitrate assimilation
Oxidoreductase

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)

Periplasm

end case

Signal
Transport

Gene Ontology [?]

GO:0009055; Molecular function: electron transfer activity.
GO:0005506; Molecular function: iron ion binding.
GO:0008940; Molecular function: nitrate reductase activity.
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
GO:0042128; Biological process: nitrate assimilation.

case <OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)

GO:0042597; Cellular component: periplasmic space.

end case

Cross-references [?]

PROSITE	PS51669; 4FE4S_MOW_BIS_MGD; 1; trigger=PRU01004;
	PS00551; MOLYBDOPTERIN_PROK_1; 1;
	PS00490; MOLYBDOPTERIN_PROK_2; 1;
	PS00932; MOLYBDOPTERIN_PROK_3; 1;
	PS51318; TAT; 0-1; trigger=PRU00648;
Pfam	PF04879; Molybdop_Fe4S4; 1;
	PF00384; Molybdopterin; 1;
	PF01568; Molydop_binding; 1;
	PF10518; TAT_signal; 0-1;
TIGRFAMs	TIGR01706; NAPA; 1;
	TIGR01409; TAT_signal_seq; 0-1;

Computed features [?]

case not <Feature:PS51318>

General

Signal; -; 1; trigger=yes;

end case

Features [?]

From: NAPA_ECOLI (P33937)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 212 219 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 W-G-[AS]-N-M-A-E-M

BINDING (Optional) 243 247 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 S-T-[YF]-x-H

BINDING (Optional) 262 264 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 [QG]-[ST]-D

BINDING (Optional) 508 509 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 S-[DE]

BINDING (Optional) 718 727 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 [ST]-[GM]-R-[VI]-[ILV]-[ED]-H-W-H-[TS]

BINDING 46 46 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C

BINDING 49 49 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C

BINDING 53 53 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C

BINDING 81 81 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C

BINDING (Optional) 83 83 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 K

BINDING (Optional) 150 150 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 Q

BINDING (Optional) 175 175 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 N

BINDING (Optional) 179 179 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 C

BINDING (Optional) 372 372 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 M

BINDING (Optional) 376 376 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 Q

BINDING (Optional) 482 482 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 N

BINDING (Optional) 531 531 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 K

BINDING (Optional) 558 558 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 D

BINDING (Optional) 794 794 /ligand="substrate [FW]

BINDING (Optional) 802 802 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 N

BINDING (Optional) 819 819 /ligand="Mo-bis(molybdopterin guanine dinucleotide)" /ligand_id="ChEBI:CHEBI:60539 K

Additional information [?]

Size range	754-950 amino acids
Related rules	None
Fusion	None

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