HAMAP rule MF_01643

General rule information [?]

Accession	MF_01643
Dates	24-JAN-2008 (Created) 1-JUN-2023 (Last updated, Version 27)

Name

PurT

Scope

Bacteria

Archaea

Template

P33221 (PURT_ECOLI)

Triggered by

HAMAP; MF_01643 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PURT

Protein name

RecName:		Full=Formate-dependent phosphoribosylglycinamide formyltransferase;
		EC 6.3.1.21;
AltName:		Full=Formate-dependent GAR transformylase;
AltName:		Full=Non-folate glycinamide ribonucleotide transformylase;
AltName:		Full=Phosphoribosylglycinamide formyltransferase 2;
AltName:		Full=5'-phosphoribosylglycinamide transformylase 2;
AltName:		Full=GAR transformylase 2;
		Short=GART 2;

Gene name

purT

Comments [?]

Function	Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.
Catalytic activity	RHEA:24829: ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate EC 6.3.1.21 PhysiologicalDirection=left-to-right (RHEA:24830)
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.
Subunit	Homodimer.
Similarity	Belongs to the PurK/PurT family.

Keywords [?]

ATP-binding
Ligase
Metal-binding
Magnesium
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0000166; Molecular function: nucleotide binding.
GO:0005524; Molecular function: ATP binding.
GO:0043815; Molecular function: phosphoribosylglycinamide formyltransferase 2 activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.

Cross-references [?]

PROSITE	PS50975; ATP_GRASP; 1;
Pfam	PF02222; ATP-grasp; 1;
NCBIfam	TIGR01142; PurT; 1;

Features [?]

case <Feature:PS50975>

From: PURT_ECOLI (P33221)

Key From To Description Tag Condition FTGroup

DOMAIN 119 308 ATP-grasp

end case

BINDING 160 165 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 S-S-G-x-G-Q

BINDING 195 198 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 E-x-x-[VIAL]

BINDING 22 23 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788 E-L

BINDING 362 363 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788 R-R

BINDING 267 267 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E

BINDING 279 279 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 [ED]

BINDING 82 82 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788 E

BINDING 114 114 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 R

BINDING 155 155 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

BINDING 203 203 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 E

BINDING 286 286 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788 D

BINDING 355 355 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788 K

Additional information [?]

Size range	379-439 amino acids
Related rules	None
Fusion	None

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