HAMAP rule MF_01643
General rule information
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Accession | MF_01643 |
Dates | 24-JAN-2008 (Created)
1-JUN-2023 (Last updated, Version 27) |
Name | PurT |
Scope(s) |
Bacteria Archaea |
Template(s) | P33221 (PURT_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01643 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | PURT |
Protein name | RecName: Full=Formate-dependent phosphoribosylglycinamide formyltransferase; EC=6.3.1.21; AltName: Full=Formate-dependent GAR transformylase; AltName: Full=Non-folate glycinamide ribonucleotide transformylase; AltName: Full=Phosphoribosylglycinamide formyltransferase 2; AltName: Full=5'-phosphoribosylglycinamide transformylase 2; AltName: Full=GAR transformylase 2; Short=GART 2; |
Gene name | Name=purT; |
Comments
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FUNCTION | Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate. |
CATALYTIC ACTIVITY | Reaction=ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate; Xref=Rhea:RHEA:24829, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286, ChEBI:CHEBI:456216; EC=6.3.1.21; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24830; |
PATHWAY | Purine metabolism; IMP biosynthesis via de novo pathway; N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (formate route): step 1/1. |
SUBUNIT | Homodimer. |
SIMILARITY | Belongs to the PurK/PurT family. |
Keywords
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Gene Ontology
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GO:0000166; Molecular function:nucleotide binding |
GO:0005524; Molecular function:ATP binding |
GO:0043815; Molecular function:phosphoribosylglycinamide formyltransferase 2 activity |
GO:0006189; Biological process:'de novo' IMP biosynthetic process |
Cross-references
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Features
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From: PURT_ECOLI (P33221) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case <Feature:PS50975> | ||||||||||||
DOMAIN | 119 | 308 | /note="ATP-grasp" | |||||||||
end case | ||||||||||||
BINDING | 160 | 165 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
S-S-G-x-G-Q | ||||||||
BINDING | 195 | 198 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-x-x-[VIAL] | ||||||||
BINDING | 22 | 23 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
E-L | ||||||||
BINDING | 362 | 363 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
R-R | ||||||||
BINDING | 267 | 267 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
BINDING | 279 | 279 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[ED] | ||||||||
BINDING | 82 | 82 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
E | ||||||||
BINDING | 114 | 114 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
BINDING | 155 | 155 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
BINDING | 203 | 203 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E | ||||||||
BINDING | 286 | 286 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
D | ||||||||
BINDING | 355 | 355 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
K |
Additional information
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Size range | 379-439 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |