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HAMAP rule MF_01643

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General rule information [?]

Accession MF_01643
Dates 24-JAN-2008 (Created)
1-JUN-2023 (Last updated, Version 27)
Name PurT
Template P33221 (PURT_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Formate-dependent phosphoribosylglycinamide formyltransferase;
AltName: Full=Formate-dependent GAR transformylase;
AltName: Full=Non-folate glycinamide ribonucleotide transformylase;
AltName: Full=Phosphoribosylglycinamide formyltransferase 2;
AltName: Full=5'-phosphoribosylglycinamide transformylase 2;
AltName: Full=GAR transformylase 2;
Short=GART 2;
Gene name

Comments [?]

Function Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.
Catalytic activity RHEA:24829: ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate
PhysiologicalDirection=left-to-right (RHEA:24830)
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.
Subunit Homodimer.
Similarity Belongs to the PurK/PurT family.

Keywords [?]

Gene Ontology [?]

GO:0000166; Molecular function: nucleotide binding.
GO:0005524; Molecular function: ATP binding.
GO:0043815; Molecular function: phosphoribosylglycinamide formyltransferase 2 activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.

Cross-references [?]

Pfam PF02222; ATP-grasp; 1;
NCBIfam TIGR01142; PurT; 1;

Features [?]

case <Feature:PS50975>
From: PURT_ECOLI (P33221)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     119     308       ATP-grasp        
end case
BINDING     160     165       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     S-S-G-x-G-Q  
BINDING     195     198       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E-x-x-[VIAL]  
BINDING     22     23       /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788     E-L  
BINDING     362     363       /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788     R-R  
BINDING     267     267       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     E  
BINDING     279     279       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     [ED]  
BINDING     82     82       /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788     E  
BINDING     114     114       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R  
BINDING     155     155       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  
BINDING     203     203       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     E  
BINDING     286     286       /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788     D  
BINDING     355     355       /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788     K  

Additional information [?]

Size range 379-439 amino acids
Related rules None
Fusion None