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HAMAP rule MF_01643

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01643
Accession MF_01643
Dates 24-JAN-2008 (Created)
03-SEP-2024 (Last updated, Version 29)
Name PurT
Scope(s) Bacteria
Archaea
Template(s) P33221; [ Recover all ]
Triggered by HAMAP; MF_01643 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PURT
Protein name RecName: Full=Formate-dependent phosphoribosylglycinamide formyltransferase;
                 EC=6.3.1.21;
AltName: Full=Formate-dependent GAR transformylase;
AltName: Full=Non-folate glycinamide ribonucleotide transformylase;
AltName: Full=Phosphoribosylglycinamide formyltransferase 2;
AltName: Full=5'-phosphoribosylglycinamide transformylase 2;
AltName: Full=GAR transformylase 2;
                 Short=GART 2;
Gene name Name=purT;

Comments [?]

FUNCTIONInvolved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.
CATALYTIC ACTIVITY Reaction=N(1)-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+); Xref=Rhea:RHEA:24829, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286, ChEBI:CHEBI:456216; EC=6.3.1.21; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24830;
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (formate route): step 1/1.
SUBUNITHomodimer.
SIMILARITYBelongs to the PurK/PurT family.

Keywords [?]

ATP-binding
Ligase
Metal-binding
Magnesium
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0000166; Molecular function:nucleotide binding
GO:0005524; Molecular function:ATP binding
GO:0043815; Molecular function:phosphoribosylglycinamide formyltransferase 2 activity
GO:0006189; Biological process:'de novo' IMP biosynthetic process

Cross-references [?]

PROSITE PS50975; ATP_GRASP; 1;
Pfam PF02222; ATP-grasp; 1;
NCBIfam TIGR01142; PurT; 1;

Features [?]

From: PURT_ECOLI (P33221)
Key From To Description Tag Condition FTGroup
case <Feature:PS50975>
DOMAIN 119 308 /note="ATP-grasp"
end case
BINDING 160 165 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 S-S-G-x-G-Q
BINDING 195 198 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E-x-x-[VIAL]
BINDING 22 23 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
/ligand_id="ChEBI:CHEBI:143788"		 E-L
BINDING 362 363 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
/ligand_id="ChEBI:CHEBI:143788"		 R-R
BINDING 267 267 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 279 279 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [ED]
BINDING 82 82 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
/ligand_id="ChEBI:CHEBI:143788"		 E
BINDING 114 114 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 R
BINDING 155 155 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 K
BINDING 203 203 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 E
BINDING 286 286 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
/ligand_id="ChEBI:CHEBI:143788"		 D
BINDING 355 355 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
/ligand_id="ChEBI:CHEBI:143788"		 K

Additional information [?]

Size range 379-439 amino acids
Related rules None
Fusion Nter: None Cter: None



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