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HAMAP rule MF_01654

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General rule information [?]

Accession MF_01654
Dates 30-APR-2008 (Created)
12-MAR-2024 (Last updated, Version 22)
Name MhpC
Scope(s) Bacteria
Template(s) P77044 (MHPC_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01654 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MHPC
Protein name RecName: Full=2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase;
AltName: Full=2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase;
AltName: Full=2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase;
AltName: Full=2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase;
Gene name Name=mhpC;

Comments [?]

FUNCTIONCatalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid.
CATALYTIC ACTIVITY Reaction=(2Z,4E)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O = (2Z)-2- hydroxypenta-2,4-dienoate + H(+) + succinate; Xref=Rhea:RHEA:34187, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031, ChEBI:CHEBI:66887, ChEBI:CHEBI:67152; EC=;
CATALYTIC ACTIVITY Reaction=(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-trienedioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate + H(+); Xref=Rhea:RHEA:34191, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29806, ChEBI:CHEBI:66888, ChEBI:CHEBI:67152; EC=;
PATHWAYAromatic compound metabolism; 3-phenylpropanoate degradation.
SIMILARITYBelongs to the AB hydrolase superfamily. MhpC family.

Keywords [?]

Gene Ontology [?]

GO:0018771; Molecular function:2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
GO:0019380; Biological process:3-phenylpropionate catabolic process

Cross-references [?]

Pfam PF00561; Abhydrolase_1; 1;

Features [?]

From: MHPC_ECOLI (P77044)
Key From To Description Tag Condition FTGroup
ACT_SITE 267 267 /note="Proton acceptor" H
SITE 114 114 /note="Transition state stabilizer" S
SITE 192 192 /note="Catalytic role in ketonization of the dienol substrate (substrate destabilization)" R

Additional information [?]

Size range 285-296 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments BURCE and COMTE are tagged as atypical due to their sequence similarity to BphD.

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