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Annotation rule MF_01675
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General rule information [?]

Accession MF_01675
Dates 7-OCT-2008 (Created)
19-NOV-2019 (Last updated, Version 13)
Name Sep_Cys_tRNA_synth
Templates Q59072 (SPSS_METJA); O30207 (SPSS1_ARCFU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
AltName: Full=Sep-tRNA:Cys-tRNA synthase;

Comments [?]

Function Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).
Catalytic activity RHEA:25686: H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate
Cofactor pyridoxal 5'-phosphate
Subunit Homodimer. Interacts with SepRS.
Similarity Belongs to the SepCysS family.

Keywords [?]

Gene Ontology [?]

GO:0043766; Molecular function: Sep-tRNA:Cys-tRNA synthase activity.

Cross-references [?]

Pfam PF05889; SLA_LP_auto_ag; 1;
TIGRFAMs TIGR02539; SepCysS; 1;

Features [?]

From: SPSS1_ARCFU (O30207)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     78     79       Pyridoxal phosphate binding     A-R  
REGION     206     208       Pyridoxal phosphate binding     S-G-H  
BINDING     183     183       Pyridoxal phosphate     N  
BINDING     79     79       Sulfur donor or Sep-tRNA(Cys)     R  
BINDING     103     103       Sulfur donor or Sep-tRNA(Cys)     H  
BINDING     104     104       Sulfur donor or Sep-tRNA(Cys)     Y  
BINDING     267     267       Sulfur donor or Sep-tRNA(Cys)     R  
BINDING (Optional)     276     276       Sulfur donor or Sep-tRNA(Cys)     [KR]  
MOD_RES     209     209       N6-(pyridoxal phosphate)lysine     K  

Additional information [?]

Size range 371-463 amino acids
Related rules None
Fusion None
Comments Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus and Methanopyrus kandleri use the SepRS?SepCysS pathway obligatorily for synthesis of Cys-tRNACys, whereas many others (for example, Methanococcus maripaludis and Archaeoglobus fulgidus) contain genes for both the CysRS and the SepRS?SepCysS pathways (PMID:16380427).