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Annotation rule MF_01675 |
Accession | MF_01675 |
Dates | 7-OCT-2008 (Created) 19-NOV-2019 (Last updated, Version 13) |
Name | Sep_Cys_tRNA_synth |
Scope | Archaea |
Templates | Q59072 (SPSS_METJA); O30207 (SPSS1_ARCFU): [Recover all] |
Triggered by |
Identifier |
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Protein name |
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Function | Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)). |
Catalytic activity | RHEA:25686: H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate
EC 2.5.1.73 |
Cofactor | pyridoxal 5'-phosphate |
Subunit | Homodimer. Interacts with SepRS. |
Similarity | Belongs to the SepCysS family. |
From: SPSS1_ARCFU (O30207) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 78 | 79 | Pyridoxal phosphate binding | A-R | ||||||||
REGION | 206 | 208 | Pyridoxal phosphate binding | S-G-H | ||||||||
BINDING | 183 | 183 | Pyridoxal phosphate | N | ||||||||
BINDING | 79 | 79 | Sulfur donor or Sep-tRNA(Cys) | R | ||||||||
BINDING | 103 | 103 | Sulfur donor or Sep-tRNA(Cys) | H | ||||||||
BINDING | 104 | 104 | Sulfur donor or Sep-tRNA(Cys) | Y | ||||||||
BINDING | 267 | 267 | Sulfur donor or Sep-tRNA(Cys) | R | ||||||||
BINDING (Optional) | 276 | 276 | Sulfur donor or Sep-tRNA(Cys) | [KR] | ||||||||
MOD_RES | 209 | 209 | N6-(pyridoxal phosphate)lysine | K |
Size range | 371-463 amino acids |
Related rules | None |
Fusion | None |
Comments | Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus and Methanopyrus kandleri use the SepRS?SepCysS pathway obligatorily for synthesis of Cys-tRNACys, whereas many others (for example, Methanococcus maripaludis and Archaeoglobus fulgidus) contain genes for both the CysRS and the SepRS?SepCysS pathways (PMID:16380427). |