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HAMAP rule MF_01675

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General rule information [?]

Accession MF_01675
Dates 7-OCT-2008 (Created)
1-JUN-2023 (Last updated, Version 18)
Name Sep_Cys_tRNA_synth
Scope(s) Archaea
Template(s) Q59072 (SPSS_METJA); O30207 (SPSS1_ARCFU); [ Recover all ]
Triggered by HAMAP; MF_01675 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SPSS
Protein name RecName: Full=O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
AltName: Full=Sep-tRNA:Cys-tRNA synthase;

Comments [?]

FUNCTIONConverts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L- cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).
CATALYTIC ACTIVITY Reaction=H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L- cysteinyl-tRNA(Cys) + phosphate; Xref=Rhea:RHEA:25686, Rhea:RHEA- COMP:9679, Rhea:RHEA-COMP:9719, ChEBI:CHEBI:15378, ChEBI:CHEBI:29919, ChEBI:CHEBI:43474, ChEBI:CHEBI:78517, ChEBI:CHEBI:78551; EC=;
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
SUBUNITHomodimer. Interacts with SepRS.
SIMILARITYBelongs to the SepCysS family.

Keywords [?]

Gene Ontology [?]

GO:0043766; Molecular function:Sep-tRNA:Cys-tRNA synthase activity

Cross-references [?]

Pfam PF05889; SLA_LP_auto_ag; 1;
NCBIfam TIGR02539; SepCysS; 1;

Features [?]

From: SPSS1_ARCFU (O30207)
Key From To Description Tag Condition FTGroup
BINDING 78 79 /ligand="pyridoxal 5'-phosphate"
BINDING 206 208 /ligand="pyridoxal 5'-phosphate"
BINDING 183 183 /ligand="pyridoxal 5'-phosphate"
MOD_RES 209 209 /note="N6-(pyridoxal phosphate)lysine" K

Additional information [?]

Size range 371-463 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus and Methanopyrus kandleri use the SepRS?SepCysS pathway obligatorily for synthesis of Cys-tRNACys, whereas many others (for example, Methanococcus maripaludis and Archaeoglobus fulgidus) contain genes for both the CysRS and the SepRS?SepCysS pathways (PMID:16380427).

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