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HAMAP rule MF_01690

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General rule information [?]

Accession MF_01690
Dates 22-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 19)
Name DapE
Scope(s) Bacteria
Pseudomonadota
Template(s) Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI); Q9JYL2 (DAPE_NEIMB); Q9KQ52 (DAPE_VIBCH); [ Recover all ]
Triggered by HAMAP; MF_01690 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DAPE
Protein name RecName: Full=Succinyl-diaminopimelate desuccinylase;
                 Short=SDAP desuccinylase;
                 EC=3.5.1.18;
AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
Gene name Name=dapE;

Comments [?]

FUNCTIONCatalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.
CATALYTIC ACTIVITY Reaction=H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)- 2,6-diaminoheptanedioate + succinate; Xref=Rhea:RHEA:22608, ChEBI:CHEBI:15377, ChEBI:CHEBI:30031, ChEBI:CHEBI:57609, ChEBI:CHEBI:58087; EC=3.5.1.18;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.;
PATHWAYAmino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
SUBUNITHomodimer.
SIMILARITYBelongs to the peptidase M20A family. DapE subfamily.

Keywords [?]


Gene Ontology [?]

GO:0008270; Molecular function:zinc ion binding
GO:0009014; Molecular function:succinyl-diaminopimelate desuccinylase activity
GO:0050897; Molecular function:cobalt ion binding
GO:0009089; Biological process:lysine biosynthetic process via diaminopimelate
GO:0019877; Biological process:diaminopimelate biosynthetic process

Cross-references [?]

PROSITE PS00758; ARGE_DAPE_CPG2_1; 1;
PROSITE PS00759; ARGE_DAPE_CPG2_2; 1;
Pfam PF07687; M20_dimer; 1;
Pfam PF01546; Peptidase_M20; 1;
NCBIfam TIGR01246; DapE_proteo; 1;
NCBIfam TIGR01910; DapE-ArgE; 1;

Features [?]

From: DAPE_HAEIN (P44514)
Key From To Description Tag Condition FTGroup
ACT_SITE 69 69 D
ACT_SITE 134 134 /note="Proton acceptor" E
BINDING 67 67 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
H
BINDING 100 100 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
D
BINDING 100 100 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
D
BINDING 135 135 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
E
BINDING 163 163 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
E
BINDING 349 349 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
H

Additional information [?]

Size range 363-425 amino acids
Related rules None
Fusion Nter: None Cter: None



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