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Annotation rule MF_01690
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General rule information [?]

Accession MF_01690
Dates 22-APR-2009 (Created)
27-JAN-2020 (Last updated, Version 14)
Name DapE
Scope
Bacteria; Proteobacteria
Templates Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI); Q9JYL2 (DAPE_NEIMB); Q9KQ52 (DAPE_VIBCH): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DAPE
Protein name
RecName: Full=Succinyl-diaminopimelate desuccinylase;
Short=SDAP desuccinylase;
EC 3.5.1.18;
AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
Gene name
dapE

Comments [?]

Function Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.
Catalytic activity RHEA:22608: H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate
EC 3.5.1.18
Cofactor Zn(2+)
Note: Binds 1 Zn(2+) ion per subunit.
Co(2+)
Note: Binds 1 Co(2+) ion per subunit.
Pathway Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
Subunit Homodimer.
Similarity Belongs to the peptidase M20A family. DapE subfamily.

Keywords [?]


Gene Ontology [?]

GO:0008270; Molecular function: zinc ion binding.
GO:0009014; Molecular function: succinyl-diaminopimelate desuccinylase activity.
GO:0050897; Molecular function: cobalt ion binding.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0019877; Biological process: diaminopimelate biosynthetic process.

Cross-references [?]

PROSITE PS00758; ARGE_DAPE_CPG2_1; 1;
PS00759; ARGE_DAPE_CPG2_2; 1;
Pfam PF07687; M20_dimer; 1;
PF01546; Peptidase_M20; 1;
TIGRFAMs TIGR01246; DapE_proteo; 1;
TIGR01910; DapE-ArgE; 1;

Features [?]

From: DAPE_HAEIN (P44514)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     69     69             D  
ACT_SITE     134     134       Proton acceptor     E  
METAL     67     67       Cobalt or zinc 1     H  
METAL     100     100       Cobalt or zinc 1     D  
METAL     100     100       Cobalt or zinc 2     D  
METAL     135     135       Cobalt or zinc 2     E  
METAL     163     163       Cobalt or zinc 1     E  
METAL     349     349       Cobalt or zinc 2     H  

Additional information [?]

Size range 363-425 amino acids
Related rules None
Fusion None