HAMAP rule MF_01690

General rule information [?]

Accession	MF_01690
Dates	22-APR-2009 (Created) 1-JUN-2023 (Last updated, Version 19)

Name

DapE

Scope

Bacteria; Pseudomonadota

Templates

Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI); Q9JYL2 (DAPE_NEIMB); Q9KQ52 (DAPE_VIBCH): [Recover all]

Triggered by

HAMAP; MF_01690 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DAPE

Protein name

RecName:		Full=Succinyl-diaminopimelate desuccinylase;
		Short=SDAP desuccinylase;
		EC 3.5.1.18;
AltName:		Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;

Gene name

dapE

Comments [?]

Function	Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.
Catalytic activity	RHEA:22608: H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate EC 3.5.1.18
Cofactor	Zn(2+) Co(2+) Note: Binds 2 Zn(2+) or Co(2+) ions per subunit.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
Subunit	Homodimer.
Similarity	Belongs to the peptidase M20A family. DapE subfamily.

Keywords [?]

Amino-acid biosynthesis
Cobalt
Diaminopimelate biosynthesis
Hydrolase
Lysine biosynthesis
Metal-binding
Zinc

Gene Ontology [?]

GO:0008270; Molecular function: zinc ion binding.
GO:0009014; Molecular function: succinyl-diaminopimelate desuccinylase activity.
GO:0050897; Molecular function: cobalt ion binding.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0019877; Biological process: diaminopimelate biosynthetic process.

Cross-references [?]

PROSITE	PS00758; ARGE_DAPE_CPG2_1; 1;
	PS00759; ARGE_DAPE_CPG2_2; 1;
Pfam	PF07687; M20_dimer; 1;
	PF01546; Peptidase_M20; 1;
NCBIfam	TIGR01246; DapE_proteo; 1;
	TIGR01910; DapE-ArgE; 1;

Features [?]

From: DAPE_HAEIN (P44514)

Key From To Description Tag Condition FTGroup

ACT_SITE 69 69 D

ACT_SITE 134 134 Proton acceptor E

BINDING 67 67 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 100 100 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 D

BINDING 100 100 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 D

BINDING 135 135 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 E

BINDING 163 163 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 E

BINDING 349 349 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 H

Additional information [?]

Size range	363-425 amino acids
Related rules	None
Fusion	None

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