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HAMAP rule MF_01690

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General rule information [?]

Accession MF_01690
Dates 22-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 19)
Name DapE
Bacteria; Pseudomonadota
Templates Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI); Q9JYL2 (DAPE_NEIMB); Q9KQ52 (DAPE_VIBCH): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Succinyl-diaminopimelate desuccinylase;
Short=SDAP desuccinylase;
AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase;
Gene name

Comments [?]

Function Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.
Catalytic activity RHEA:22608: H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) or Co(2+) ions per subunit.
Pathway Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
Subunit Homodimer.
Similarity Belongs to the peptidase M20A family. DapE subfamily.

Keywords [?]

Gene Ontology [?]

GO:0008270; Molecular function: zinc ion binding.
GO:0009014; Molecular function: succinyl-diaminopimelate desuccinylase activity.
GO:0050897; Molecular function: cobalt ion binding.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0019877; Biological process: diaminopimelate biosynthetic process.

Cross-references [?]

PS00759; ARGE_DAPE_CPG2_2; 1;
Pfam PF07687; M20_dimer; 1;
PF01546; Peptidase_M20; 1;
NCBIfam TIGR01246; DapE_proteo; 1;
TIGR01910; DapE-ArgE; 1;

Features [?]

From: DAPE_HAEIN (P44514)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     69     69             D  
ACT_SITE     134     134       Proton acceptor     E  
BINDING     67     67       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     100     100       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     D  
BINDING     100     100       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     D  
BINDING     135     135       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     E  
BINDING     163     163       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     E  
BINDING     349     349       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     H  

Additional information [?]

Size range 363-425 amino acids
Related rules None
Fusion None