Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
HAMAP rule MF_01690
General rule information
[?]
| Accession | MF_01690 |
| Dates | 22-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 19) |
| Name | DapE |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI); Q9JYL2 (DAPE_NEIMB); Q9KQ52 (DAPE_VIBCH); [ Recover all ] |
| Triggered by |
HAMAP; MF_01690 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | DAPE |
| Protein name | RecName: Full=Succinyl-diaminopimelate desuccinylase; Short=SDAP desuccinylase; EC=3.5.1.18; AltName: Full=N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase; |
| Gene name | Name=dapE; |
Comments
[?]
| FUNCTION | Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls. |
| CATALYTIC ACTIVITY | Reaction=H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)- 2,6-diaminoheptanedioate + succinate; Xref=Rhea:RHEA:22608, ChEBI:CHEBI:15377, ChEBI:CHEBI:30031, ChEBI:CHEBI:57609, ChEBI:CHEBI:58087; EC=3.5.1.18; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.; |
| PATHWAY | Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the peptidase M20A family. DapE subfamily. |
Keywords
[?]
| Amino-acid biosynthesis |
| Cobalt |
| Diaminopimelate biosynthesis |
| Hydrolase |
| Lysine biosynthesis |
| Metal-binding |
| Zinc |
Gene Ontology
[?]
| GO:0008270; Molecular function:zinc ion binding |
| GO:0009014; Molecular function:succinyl-diaminopimelate desuccinylase activity |
| GO:0050897; Molecular function:cobalt ion binding |
| GO:0009089; Biological process:lysine biosynthetic process via diaminopimelate |
| GO:0019877; Biological process:diaminopimelate biosynthetic process |
Cross-references
[?]
| PROSITE | PS00758; ARGE_DAPE_CPG2_1; 1; |
| PROSITE | PS00759; ARGE_DAPE_CPG2_2; 1; |
| Pfam | PF07687; M20_dimer; 1; |
| Pfam | PF01546; Peptidase_M20; 1; |
| NCBIfam | TIGR01246; DapE_proteo; 1; |
| NCBIfam | TIGR01910; DapE-ArgE; 1; |
Features
[?]
| From: DAPE_HAEIN (P44514) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 69 | 69 | D | |||||||||
| ACT_SITE | 134 | 134 | /note="Proton acceptor" | E | ||||||||
| BINDING | 67 | 67 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | ||||||||
| BINDING | 100 | 100 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
D | ||||||||
| BINDING | 100 | 100 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
D | ||||||||
| BINDING | 135 | 135 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
E | ||||||||
| BINDING | 163 | 163 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
E | ||||||||
| BINDING | 349 | 349 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
H | ||||||||
Additional information
[?]
| Size range | 363-425 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |