HAMAP rule MF_01697
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01697 |
| Accession | MF_01697 |
| Dates | 20-SEP-2010 (Created)
03-SEP-2024 (Last updated, Version 16) |
| Name | MshC |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | A0QZY0; P9WJM9; [ Recover all ] |
| Triggered by |
HAMAP; MF_01697 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MSHC |
| Protein name | RecName: Full=L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase; Short=L-Cys:GlcN-Ins ligase; EC=6.3.1.13; AltName: Full=Mycothiol ligase; Short=MSH ligase; |
| Gene name | Name=mshC; |
Comments
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| FUNCTION | Catalyzes the ATP-dependent condensation of GlcN-Ins and L- cysteine to form L-Cys-GlcN-Ins. |
| CATALYTIC ACTIVITY | Reaction=1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside + L- cysteine + ATP = 1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha- D-glucopyranoside + AMP + diphosphate + H(+); Xref=Rhea:RHEA:26176, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:58886, ChEBI:CHEBI:58887, ChEBI:CHEBI:456215; EC=6.3.1.13; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily. |
Keywords
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Gene Ontology
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| GO:0008270; Molecular function:zinc ion binding |
| GO:0035446; Molecular function:cysteine-glucosaminylinositol ligase activity |
| GO:0010125; Biological process:mycothiol biosynthetic process |
Cross-references
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| Pfam | PF01406; tRNA-synt_1e; 1; |
| PRINTS | PR00983; TRNASYNTHCYS; 1; |
| NCBIfam | TIGR03447; Mycothiol_MshC; 1; |
Features
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| From: MSHC_MYCS2 (A0QZY0) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 43 | 46 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
C-G-I-T | ||||||||
| BINDING | 81 | 83 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
N-x-T | ||||||||
| BINDING | 249 | 251 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
G-x-D | ||||||||
| MOTIF | 45 | 55 | /note="'HIGH' region" | I-T-P-Y-D-[AS]-[AT]-H-[ILM]-G-H | ||||||||
| MOTIF | 187 | 192 | /note="'ERGGDP' region" | [EDQ]-[HR]-G-G-D-P | ||||||||
| MOTIF | 289 | 293 | /note="'KMSKS' region" | K-M-S-K-S | ||||||||
| BINDING | 43 | 43 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 231 | 231 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 256 | 256 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 58 | 58 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
[ST] | ||||||||
| BINDING | 227 | 227 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
W | ||||||||
| BINDING | 283 | 283 | /ligand="L-cysteinyl-5'-AMP" /ligand_id="ChEBI:CHEBI:144924" |
[LIV] | ||||||||
Additional information
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| Size range | 384-520 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |