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HAMAP rule MF_01818

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01818
Accession MF_01818
Dates 28-FEB-2005 (Created)
01-JUN-2023 (Last updated, Version 22)
Name RNase_Z_BN
Scope(s) Bacteria
Archaea
Template(s) P0A8V0; Q58897; P54548; [ Recover all ]
Triggered by HAMAP; MF_01818 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Enterobacterales>
Identifier RBN
Protein name RecName: Full=Ribonuclease BN;
                 Short=RNase BN;
                 EC=3.1.-.-;
AltName: Full=Ribonuclease Z homolog;
                 Short=RNase Z homolog;
Gene name Name=rbn;
else
Identifier RNZ
Protein name RecName: Full=Ribonuclease Z;
                 Short=RNase Z;
                 EC=3.1.26.11;
AltName: Full=tRNA 3 endonuclease;
AltName: Full=tRNase Z;
Gene name Name=rnz;
end case

Comments [?]

case <OC:Enterobacterales>
FUNCTIONZinc phosphodiesterase, which has both exoribonuclease and endoribonuclease activities.
else
FUNCTIONZinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
CATALYTIC ACTIVITY Reaction=Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.; EC=3.1.26.11;
end case
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions.;
end case
SUBUNITHomodimer.
case <OC:Enterobacterales>
SIMILARITYBelongs to the RNase Z family. RNase BN subfamily.
else
SIMILARITYBelongs to the RNase Z family.
end case

Keywords [?]

tRNA processing
Hydrolase
Nuclease
Endonuclease
case <OC:Enterobacterales>
Exonuclease
end case
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
case <OCellular component:Enterobacterales>
GO:0004519; Molecular function:endonuclease activity
GO:0004527; Molecular function:exonuclease activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0042781; Molecular function:3'-tRNA processing endoribonuclease activity
end case
GO:0008033; Biological process:tRNA processing

Cross-references [?]

Pfam PF00753; Lactamase_B; 1;
NCBIfam TIGR02651; RNase_Z; 1;
NCBIfam TIGR02649; True_RNase_BN; 1;

Features [?]

From: RNZ_BACSU (P54548)
Key From To Description Tag Condition FTGroup
ACT_SITE 67 67 /note="Proton acceptor" D
BINDING 63 63 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="catalytic"		 H 1
BINDING 65 65 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="catalytic"		 H 1
BINDING 67 67 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 D 1
BINDING 68 68 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 H 1
BINDING 140 140 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="catalytic"		 H 1
BINDING 211 211 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="catalytic"		 D 1
BINDING 211 211 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 D 1
BINDING 269 269 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 H 1

Additional information [?]

Size range 220-407 amino acids
Related rules None
Fusion Nter: None Cter: <Unknown>
Comments Was initially thought to be an arylsulfatase, given its similarity with the atsA family.



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