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HAMAP rule MF_01825

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General rule information [?]

Accession MF_01825
Dates 19-MAR-2004 (Created)
19-NOV-2022 (Last updated, Version 19)
Name PdxB
Scope(s) Bacteria
Bacteroidota
Gammaproteobacteria
Template(s) P05459 (PDXB_ECOLI); Q9I3W9 (PDXB_PSEAE); [ Recover all ]
Triggered by HAMAP; MF_01825 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PDXB
Protein name RecName: Full=Erythronate-4-phosphate dehydrogenase;
                 EC=1.1.1.290;
Gene name Name=pdxB;

Comments [?]

FUNCTIONCatalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate.
CATALYTIC ACTIVITY Reaction=4-phospho-D-erythronate + NAD(+) = (R)-3-hydroxy-2-oxo-4- phosphooxybutanoate + H(+) + NADH; Xref=Rhea:RHEA:18829, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58538, ChEBI:CHEBI:58766; EC=1.1.1.290;
PATHWAYCofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Keywords [?]


Gene Ontology [?]

GO:0016491; Molecular function:oxidoreductase activity
GO:0008615; Biological process:pyridoxine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS00065; D_2_HYDROXYACID_DH_1; 1;
PROSITE PS00670; D_2_HYDROXYACID_DH_2; 1;
PROSITE PS00671; D_2_HYDROXYACID_DH_3; 1;
Pfam PF00389; 2-Hacid_dh; 1;
Pfam PF02826; 2-Hacid_dh_C; 1;
PRINTS PR00411; PNDRDTASEI; 1;

Features [?]

From: PDXB_PSEAE (Q9I3W9)
Key From To Description Tag Condition FTGroup
BINDING 126 127 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
Q-V
BINDING 206 208 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
A-S-R
ACT_SITE 208 208 R
ACT_SITE 237 237 E
ACT_SITE 254 254 /note="Proton donor" H
BINDING 45 45 /ligand="substrate" [ST]
BINDING 66 66 /ligand="substrate" T
BINDING 146 146 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
D
BINDING 175 175 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
T
BINDING 232 232 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
D
BINDING 257 257 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
G
BINDING 258 258 /ligand="substrate" Y

Additional information [?]

Size range 345-393 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The pyridoxal phosphate can be synthetised from two separate branches: the pdxA branch (that requires the gapA (or epd), pdxB, serC and pdxA enzymes) and the pdxJ branch (that requires the dxs and pdxJ enzymes) that start different precursors. Both branches for the last step (pdxH) of the from pyridoxal phosphate pathway.



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