Home  |  Contact
Annotation rule MF_01825
Send feedback

General rule information [?]

Accession MF_01825
Dates 19-MAR-2004 (Created)
19-NOV-2019 (Last updated, Version 16)
Name PdxB
Scope
Bacteria; Bacteroidetes
Bacteria; Gammaproteobacteria
Templates P05459 (PDXB_ECOLI); Q9I3W9 (PDXB_PSEAE): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PDXB
Protein name
RecName: Full=Erythronate-4-phosphate dehydrogenase;
EC 1.1.1.290;
Gene name
pdxB

Comments [?]

Function Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate.
Catalytic activity RHEA:18829: 4-phospho-D-erythronate + NAD(+) = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + H(+) + NADH
EC 1.1.1.290
Pathway Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Keywords [?]


Gene Ontology [?]

GO:0016491; Molecular function: oxidoreductase activity.
GO:0008615; Biological process: pyridoxine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00065; D_2_HYDROXYACID_DH_1; 1;
PS00670; D_2_HYDROXYACID_DH_2; 1;
PS00671; D_2_HYDROXYACID_DH_3; 1;
Pfam PF00389; 2-Hacid_dh; 1;
PF02826; 2-Hacid_dh_C; 1;
PRINTS PR00411; PNDRDTASEI; 1;

Features [?]

From: PDXB_PSEAE (Q9I3W9)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     126     127       NAD     Q-V  
NP_BIND (Optional)     206     208       NAD     A-S-R  
ACT_SITE     208     208             R  
ACT_SITE     237     237             E  
ACT_SITE     254     254       Proton donor     H  
BINDING (Optional)     45     45       Substrate     [ST]  
BINDING (Optional)     66     66       Substrate     T  
BINDING (Optional)     146     146       NAD     D  
BINDING (Optional)     175     175       NAD; via carbonyl oxygen     T  
BINDING (Optional)     232     232       NAD     D  
BINDING (Optional)     257     257       NAD; via amide nitrogen     G  
BINDING (Optional)     258     258       Substrate     Y  

Additional information [?]

Size range 345-393 amino acids
Related rules None
Fusion None
Comments The pyridoxal phosphate can be synthetised from two separate branches: the pdxA branch (that requires the gapA (or epd), pdxB, serC and pdxA enzymes) and the pdxJ branch (that requires the dxs and pdxJ enzymes) that start different precursors. Both branches for the last step (pdxH) of the from pyridoxal phosphate pathway.