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Annotation rule MF_01864
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General rule information [?]

Accession MF_01864
Dates 9-APR-2009 (Created)
3-APR-2020 (Last updated, Version 15)
Name tRNA_metthiotr_MiaB
Scope
Bacteria
Templates P0AEI1 (MIAB_ECOLI); Q9RCI2 (MIAB_SALTY); Q9WZC1 (MIAB_THEMA); O31778 (MIAB_BACSU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MIAB
Protein name
RecName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase;
EC 2.8.4.3;
AltName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
AltName: Full=tRNA-i(6)A37 methylthiotransferase;
Gene name
miaB

Comments [?]

Function Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity RHEA:37067: [sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine
EC 2.8.4.3
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the methylthiotransferase family. MiaB subfamily.

Keywords [?]


Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS50926; TRAM; 0-1; trigger=PRU00208;
PS01278; MTTASE_RADICAL; 1;
PS51449; MTTASE_N; 1; trigger=PRU00780;
PS51918; RADICAL_SAM; 1; trigger=PRU01266;
Pfam PF04055; Radical_SAM; 1;
PF01938; TRAM; 0-1;
PF00919; UPF0004; 1;
TIGRFAMs TIGR01574; MiaB-methiolase; 1;
TIGR00089; TIGR00089; 1;

Features [?]

From: MIAB_ECOLI (P0AEI1)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     12     12       Iron-sulfur (4Fe-4S)     C   1
METAL     49     49       Iron-sulfur (4Fe-4S)     C   1
METAL     83     83       Iron-sulfur (4Fe-4S)     C   1
METAL     157     157       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2
METAL     161     161       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2
METAL     164     164       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2

Additional information [?]

Size range 418-540 amino acids
Related rules None
Fusion Nter: None; Cter: <Unknown>