HAMAP rule MF_01864

General rule information [?]

Accession	MF_01864
Dates	9-APR-2009 (Created) 1-JUN-2023 (Last updated, Version 20)

Name	tRNA_metthiotr_MiaB

Scope

Bacteria

Templates

P0AEI1 (MIAB_ECOLI); Q9RCI2 (MIAB_SALTY); Q9WZC1 (MIAB_THEMA); O31778 (MIAB_BACSU): [Recover all]

Triggered by

HAMAP; MF_01864 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MIAB

Protein name

RecName:		Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase;
		EC 2.8.4.3;
AltName:		Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
AltName:		Full=tRNA-i(6)A37 methylthiotransferase;

Gene name

miaB

Comments [?]

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity	RHEA:37067: [sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine EC 2.8.4.3
Cofactor	[4Fe-4S] cluster Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Subunit	Monomer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the methylthiotransferase family. MiaB subfamily.

Keywords [?]

4Fe-4S
Cytoplasm
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
Transferase
tRNA processing

Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS50926; TRAM; 0-1; trigger=PRU00208;
	PS01278; MTTASE_RADICAL; 1;
	PS51449; MTTASE_N; 1; trigger=PRU00780;
	PS51918; RADICAL_SAM; 1; trigger=PRU01266;
Pfam	PF04055; Radical_SAM; 1;
	PF01938; TRAM; 0-1;
	PF00919; UPF0004; 1;
NCBIfam	TIGR01574; MiaB-methiolase; 1;
	TIGR00089; TIGR00089; 1;

Features [?]

From: MIAB_ECOLI (P0AEI1)

Key From To Description Tag Condition FTGroup

BINDING 12 12 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 49 49 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 83 83 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 157 157 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

BINDING 161 161 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

BINDING 164 164 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

Additional information [?]

Size range	418-540 amino acids
Related rules	None
Fusion	Nter: None; Cter: <Unknown>

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