HAMAP rule MF_01864
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01864 |
| Accession | MF_01864 |
| Dates | 09-APR-2009 (Created)
03-DEC-2024 (Last updated, Version 23) |
| Name | tRNA_metthiotr_MiaB |
| Scope(s) |
Bacteria |
| Template(s) | P0AEI1; Q9RCI2; Q9WZC1; O31778; [ Recover all ] |
| Triggered by |
HAMAP; MF_01864 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MIAB |
| Protein name | RecName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; EC=2.8.4.3; AltName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase; |
| Gene name | Name=miaB; |
Comments
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| FUNCTION | Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. |
| CATALYTIC ACTIVITY | Reaction=N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)- dimethylallyladenosine(37) in tRNA + (sulfur carrier)-H + 5'- deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+); Xref=Rhea:RHEA:37067, Rhea:RHEA-COMP:10375, Rhea:RHEA- COMP:10376, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:29917, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:74415, ChEBI:CHEBI:74417; EC=2.8.4.3; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the methylthiotransferase family. MiaB subfamily. |
Keywords
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Gene Ontology
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| GO:0016740; Molecular function:transferase activity |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
| GO:0006400; Biological process:tRNA modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS50926; TRAM; 0-1; |
| PROSITE | PS01278; MTTASE_RADICAL; 1; |
| PROSITE | PS51449; MTTASE_N; 1; |
| PROSITE | PS51918; RADICAL_SAM; 1; |
| Pfam | PF04055; Radical_SAM; 1; |
| Pfam | PF01938; TRAM; 0-1; |
| Pfam | PF00919; UPF0004; 1; |
| NCBIfam | TIGR01574; MiaB-methiolase; 1; |
| NCBIfam | TIGR00089; TIGR00089; 1; |
Features
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| From: MIAB_ECOLI (P0AEI1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 12 | 12 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
| BINDING | 49 | 49 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
| BINDING | 83 | 83 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
| BINDING | 157 | 157 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
| BINDING | 161 | 161 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
| BINDING | 164 | 164 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
Additional information
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| Size range | 418-540 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: <Unknown> |