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Annotation rule MF_01873
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General rule information [?]

Accession MF_01873
Dates 15-OCT-2009 (Created)
19-NOV-2019 (Last updated, Version 14)
Name 23SrRNA_methyltr_Cfr
Bacteria; Firmicutes
Templates Q9FBG4 (CFR_STASC); A5HBL2 (CFR_STAAU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribosomal RNA large subunit methyltransferase Cfr;
AltName: Full=23S rRNA (adenine(2503)-C(8))-methyltransferase;
AltName: Full=23S rRNA m8A2503 methyltransferase;
Gene name

Comments [?]

Function Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics.
Catalytic activity RHEA:42632: adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 5'-deoxyadenosine + 8-methyladenosine(2503) in 23S rRNA + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
end case
Subcellular location Cytoplasm.
Miscellaneous Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.
Similarity Belongs to the radical SAM superfamily. RlmN family. Cfr subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0019843; Molecular function: rRNA binding.
GO:0016433; Molecular function: rRNA (adenine) methyltransferase activity.
case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0070475; Biological process: rRNA base methylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
PIRSF PIRSF006004; CHP00048; 1;
TIGRFAMs TIGR04432; rSAM_Cfr; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
REGION     158     159       S-adenosyl-L-methionine binding     G-E  
REGION     212     214       S-adenosyl-L-methionine binding     S-x-H  
ACT_SITE     91     91       Proton acceptor     E  
ACT_SITE     338     338       S-methylcysteine intermediate     C  
METAL     112     112       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1
METAL     116     116       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1
METAL     119     119       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1
BINDING     189     189       S-adenosyl-L-methionine     S  
BINDING     293     293       S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen     N  
DISULFID     105     338       (transient)     C-x*-C  

Additional information [?]

Size range 343-350 amino acids
Related rules MF_01849 (RLMN)
Fusion None