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HAMAP rule MF_01873

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General rule information [?]

Accession MF_01873
Dates 15-OCT-2009 (Created)
1-JUN-2023 (Last updated, Version 20)
Name 23SrRNA_methyltr_Cfr
Scope(s) Bacteria
Template(s) Q9FBG4 (CFR_MAMSC); A5HBL2 (CFR_STAAU); [ Recover all ]
Triggered by HAMAP; MF_01873 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CFR
Protein name RecName: Full=Ribosomal RNA large subunit methyltransferase Cfr;
AltName: Full=23S rRNA (adenine(2503)-C(8))-methyltransferase;
AltName: Full=23S rRNA m8A2503 methyltransferase;
Gene name Name=cfr;

Comments [?]

FUNCTIONSpecifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics.
CATALYTIC ACTIVITY Reaction=adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 5'-deoxyadenosine + 8- methyladenosine(2503) in 23S rRNA + L-methionine + 2 oxidized [2Fe- 2S]-[ferredoxin] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42632, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10152, Rhea:RHEA-COMP:10153, ChEBI:CHEBI:17319, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74543; EC=;
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
MISCELLANEOUSReaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.
SIMILARITYBelongs to the radical SAM superfamily. RlmN family. Cfr subfamily.

Keywords [?]

Gene Ontology [?]

GO:0019843; Molecular function:rRNA binding
GO:0016433; Molecular function:rRNA (adenine) methyltransferase activity
case <FTGroup:1>
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
end case
GO:0070475; Biological process:rRNA base methylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
PIRSF PIRSF006004; CHP00048; 1;
NCBIfam TIGR04432; rSAM_Cfr; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 158 159 /ligand="S-adenosyl-L-methionine"
BINDING 212 214 /ligand="S-adenosyl-L-methionine"
ACT_SITE 91 91 /note="Proton acceptor" E
ACT_SITE 338 338 /note="S-methylcysteine intermediate" C
BINDING 112 112 /ligand="[4Fe-4S] cluster"
C 1
BINDING 116 116 /ligand="[4Fe-4S] cluster"
C 1
BINDING 119 119 /ligand="[4Fe-4S] cluster"
C 1
BINDING 189 189 /ligand="S-adenosyl-L-methionine"
BINDING 293 293 /ligand="S-adenosyl-L-methionine"
DISULFID 105 338 /note="(transient)" C-x*-C

Additional information [?]

Size range 343-350 amino acids
Related rules MF_01849
Fusion Nter: None Cter: None

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