HAMAP rule MF_01873
General rule information
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Accession | MF_01873 |
Dates | 15-OCT-2009 (Created)
1-JUN-2023 (Last updated, Version 20) |
Name | 23SrRNA_methyltr_Cfr |
Scope(s) |
Bacteria Bacillota |
Template(s) | Q9FBG4 (CFR_MAMSC); A5HBL2 (CFR_STAAU); [ Recover all ] |
Triggered by |
HAMAP; MF_01873 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | CFR |
Protein name | RecName: Full=Ribosomal RNA large subunit methyltransferase Cfr; EC=2.1.1.224; AltName: Full=23S rRNA (adenine(2503)-C(8))-methyltransferase; AltName: Full=23S rRNA m8A2503 methyltransferase; |
Gene name | Name=cfr; |
Comments
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FUNCTION | Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics. |
CATALYTIC ACTIVITY | Reaction=adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 5'-deoxyadenosine + 8- methyladenosine(2503) in 23S rRNA + L-methionine + 2 oxidized [2Fe- 2S]-[ferredoxin] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42632, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10152, Rhea:RHEA-COMP:10153, ChEBI:CHEBI:17319, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74543; EC=2.1.1.224; |
case <FTGroup:1> | |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
end case | |
SUBCELLULAR LOCATION | Cytoplasm. |
MISCELLANEOUS | Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue. |
SIMILARITY | Belongs to the radical SAM superfamily. RlmN family. Cfr subfamily. |
Keywords
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Antibiotic resistance | |
Cytoplasm | |
Disulfide bond | |
Methyltransferase | |
rRNA processing | |
S-adenosyl-L-methionine | |
Transferase | |
case <FTGroup:1> | |
4Fe-4S | |
Iron | |
Iron-sulfur | |
Metal-binding | |
end case |
Gene Ontology
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GO:0019843; Molecular function:rRNA binding | |
GO:0016433; Molecular function:rRNA (adenine) methyltransferase activity | |
case <FTGroup:1> | |
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
end case | |
GO:0070475; Biological process:rRNA base methylation | |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF04055; Radical_SAM; 1; |
PIRSF | PIRSF006004; CHP00048; 1; |
NCBIfam | TIGR04432; rSAM_Cfr; 1; |
Features
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From: CFR_MAMSC (Q9FBG4) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 158 | 159 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G-E | ||||||||
BINDING | 212 | 214 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S-x-H | ||||||||
ACT_SITE | 91 | 91 | /note="Proton acceptor" | E | ||||||||
ACT_SITE | 338 | 338 | /note="S-methylcysteine intermediate" | C | ||||||||
BINDING | 112 | 112 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
BINDING | 116 | 116 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
BINDING | 119 | 119 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
BINDING | 189 | 189 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S | ||||||||
BINDING | 293 | 293 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
N | ||||||||
DISULFID | 105 | 338 | /note="(transient)" | C-x*-C |
Additional information
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Size range | 343-350 amino acids |
Related rules |
MF_01849 |
Fusion | Nter: None Cter: None |