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HAMAP rule MF_01886
General rule information
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| Accession | MF_01886 |
| Dates | 4-NOV-2010 (Created)
19-NOV-2022 (Last updated, Version 12) |
| Name | tRNA_acetyltr_TmcA |
| Scope(s) |
Bacteria Gammaproteobacteria Archaea |
| Template(s) | P76562 (TMCA_ECOLI); D4GW73 (TMCA_HALVD); [ Recover all ] |
| Triggered by |
HAMAP; MF_01886 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TMCA |
| Protein name | RecName: Full=tRNA(Met) cytidine acetyltransferase TmcA; EC=2.3.1.193; |
| Gene name | Name=tmcA; |
Comments
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| FUNCTION | Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + ATP + cytidine(34) in elongator tRNA(Met) + H2O = ADP + CoA + H(+) + N(4)-acetylcytidine(34) in elongator tRNA(Met) + phosphate; Xref=Rhea:RHEA:43788, Rhea:RHEA-COMP:10693, Rhea:RHEA- COMP:10694, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216; EC=2.3.1.193; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the TmcA family. |
Keywords
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| Acyltransferase |
| ATP-binding |
| Cytoplasm |
| Nucleotide-binding |
| RNA-binding |
| Transferase |
| tRNA processing |
| tRNA-binding |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0000049; Molecular function:tRNA binding |
| GO:0051392; Molecular function:tRNA N-acetyltransferase activity |
| GO:0051391; Biological process:tRNA acetylation |
| GO:0002101; Biological process:tRNA wobble cytosine modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS51186; GNAT; 1; |
| Pfam | PF08351; DUF1726; 1; |
| Pfam | PF05127; DUF699; 1; |
| Pfam | PF00583; Acetyltransf_1; 1; |
Features
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| From: TMCA_ECOLI (P76562) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 174 | 175 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-A | ||||||||
| BINDING | 202 | 211 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-R-G-K-S-A-L-A-G-Q | ||||||||
| BINDING | 461 | 463 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
[IV]-A-[VT] | ||||||||
| BINDING | 468 | 474 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
Q-R-E-G-T-G-R | ||||||||
| BINDING | 180 | 180 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Q | ||||||||
| BINDING | 319 | 319 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
| BINDING | 499 | 499 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
E | ||||||||
| BINDING | 506 | 506 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
R | ||||||||
Additional information
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| Size range | 596-868 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |