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Annotation rule MF_01930
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General rule information [?]

Accession MF_01930
Dates 1-SEP-2014 (Created)
23-MAR-2020 (Last updated, Version 5)
Name PurN
Scope
Bacteria
Template P08179 (PUR3_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PUR3
Protein name
RecName: Full=Phosphoribosylglycinamide formyltransferase;
EC 2.1.2.2;
AltName: Full=5'-phosphoribosylglycinamide transformylase;
AltName: Full=GAR transformylase;
Short=GART;
Gene name
purN

Comments [?]

Function Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.
Catalytic activity RHEA:15053: (6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide
EC 2.1.2.2
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Similarity Belongs to the GART family.

Keywords [?]


Gene Ontology [?]

GO:0004644; Molecular function: phosphoribosylglycinamide formyltransferase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.

Cross-references [?]

PROSITE PS00373; GART; 1;
Pfam PF00551; Formyl_trans_N; 1;
TIGRFAMs TIGR00639; PurN; 1;

Features [?]

From: PUR3_ECOLI (P08179)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     11     13       5'-phosphoribosylglycinamide binding     G-[ST]-N  
REGION (Optional)     89     92       10-formyltetrahydrofolate binding     [MLF]-[RK]-[IL]-[LVI]  
ACT_SITE     108     108       Proton donor     H  
BINDING (Optional)     64     64       10-formyltetrahydrofolate     [RK]  
BINDING (Optional)     106     106       10-formyltetrahydrofolate     N  
SITE (Optional)     144     144       Raises pKa of active site His     D  

Additional information [?]

Size range 169-270 amino acids
Related rules None
Fusion None