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HAMAP rule MF_01930

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General rule information [?]

Accession MF_01930
Dates 1-SEP-2014 (Created)
18-JUL-2023 (Last updated, Version 10)
Name PurN
Scope(s) Bacteria
Template(s) P08179 (PUR3_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01930 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PUR3
Protein name RecName: Full=Phosphoribosylglycinamide formyltransferase;
AltName: Full=5'-phosphoribosylglycinamide transformylase;
AltName: Full=GAR transformylase;
Gene name Name=purN;

Comments [?]

FUNCTIONCatalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.
CATALYTIC ACTIVITY Reaction=(6R)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D- ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)- formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide; Xref=Rhea:RHEA:15053, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286, ChEBI:CHEBI:195366; EC=;
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (10-formyl THF route): step 1/1.
SIMILARITYBelongs to the GART family.

Keywords [?]

Gene Ontology [?]

GO:0004644; Molecular function:phosphoribosylglycinamide formyltransferase activity
GO:0006189; Biological process:'de novo' IMP biosynthetic process

Cross-references [?]

Pfam PF00551; Formyl_trans_N; 1;
NCBIfam TIGR00639; PurN; 1;

Features [?]

From: PUR3_ECOLI (P08179)
Key From To Description Tag Condition FTGroup
BINDING 11 13 /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
BINDING 89 92 /ligand="(6R)-10-formyltetrahydrofolate"
ACT_SITE 108 108 /note="Proton donor" H
BINDING 64 64 /ligand="(6R)-10-formyltetrahydrofolate"
BINDING 106 106 /ligand="(6R)-10-formyltetrahydrofolate"
SITE 144 144 /note="Raises pKa of active site His" D

Additional information [?]

Size range 169-270 amino acids
Related rules None
Fusion Nter: None Cter: None

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